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We narrowed to 70 results for: SEU

Showing: 1 - 30 of 70 results

  1. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ... lentiviral vector will infect, pseudotyping can help. In pseudotyping, you produce viral vectors using..., retrovirus, and rabies virus. How to pseudotype To pseudotype your viral vector, you use the same protocol...Broad tropism: VSV-G pseudotyping A commonly used envelope protein for pseudotyping is VSV-G, or vesicular...proteins for pseudotyping While VSV-G is the most commonly used envelope protein for pseudotyping, there are...Other pseudotyping options There are many, many other options for envelope proteins for pseudotyping that...restrict or broaden the host cell range (tropism). Pseudotyping is only done on enveloped viruses such as lentivirus...glycoprotein instead of the wild-type glycoprotein. The pseudotyped protein is encoded on the envelope plasmid which...

  2. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type
    Blog Post
    ...neutralization rates of B.1.351 pseudovirus was similar to that of pseudoviruses representing a bat-derived...Figure 1: Neutralization levels against spike pseudovirus decreases depending on the variant. The lowest...2021 with permission.   The spike variant pseudovirus neutralization assay Balazs’s lab, which studies...circulating variants. This allowed us to create pseudoviruses that represent lineages and variants that are...sera was used in the high-throughput SARS-CoV-2 pseudovirus neutralization assay. Neutralization was quantified...via human ACE2 receptors. In their assay, the pseudoviruses express a luciferase reporter which is used ...ACE2-expressing target cells. Any neutralized pseudoviruses would be unable to get into the target cells...

  3. SARS-CoV-2 Pseudotyped Virus

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    Collection
    ...Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein Nemazee lab - Pseudotyped virus ...Viral Vectors 101: Pseudotyping Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV...for viral pseudotyping with SARS-CoV-2 spike protein. Collections... Collections COVID-19 - Viral Pseudotyping SARS-CoV...SARS-CoV-2 Pseudotyped Virus Mechanisms of SARS-CoV-2 infection remain poorly understood but are critical for...virus. Alternatively, it is possible to generate pseudovirus, where the structural and functional components...spike (S) glycoprotein on the envelope surface. Pseudotyped virus particles can then be used to infect permissive...

  4. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...Careers and Rachel Casseus, Esq. Founder of Casseus Law. Rachel Casseus Esq., Founder Casseus Law. Driven by...Celano of Propel Careers and Rachel Casseus, Esq. Founder of Casseus Law. Disclaimer: The contents of this...and EB-1B cases, Casseus Law recommends 6-8 letters.  For these recommendations, Casseus Law suggests a ...learn more, visit www.propelcareers.com. Casseus Law: Casseus Law is dedicated to the practice of US immigration...law, Casseus Law provides legal services to people located in all 50 states and globally. Casseus Law .... Merely being asked to perform is not enough. Casseus Law suggests that if you are not already reviewing... usually only support tenure track professors. Casseus Law has seen that research institutions are willing...

  5. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...developed ways to pseudotype the spike protein onto another virus. With the pseudovirus, scientists can ...that can be incorporated into pseudoviruses. The lab used these pseudoviruses to determine how effective ...plasmids for SARS-CoV-2 spike pseudotyping, visit the SARS-CoV-2 Pseudotyped Virus collection page. SARS-CoV... in other labs. Spike protein, variants, and pseudotyping The SARS-CoV-2 virus depends on its spike protein...’s lab published a protocol and reagents for pseudotyping lentivirus with the SARS-CoV-2 spike protein... the spike protein to improve expression and pseudotyping efficiency. This plasmid was later used by another...Alejandro Balazs’s lab includes those for generating pseudovirus and those encoding different spike protein variants...

  6. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...regulated in the medicinal plant, the Catharanthus roseus (Figure 1). The ultimate goal of our research is... in the plant or in plant tissue cultures.   C. roseus is not a model organism such as Arabidopsis or ...or tobacco. Studying gene function in C. roseus is strongly limited by the availability of methods. Infection...tissue culture. This method is commonly used in C. roseus, but processes specific to photosynthetic active... and Budak, 2009). This method works well in C. roseus. A common method for overexpressing genes In plants...interest is transferred into the cells. But for C. roseus, Agrobacterium infiltration into leaves does not...infiltrate. We have recently overcome this problem in C. roseus by working with young seedlings and optimizing ...

  7. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...mentioned museums. Indianapolis is home to one of "The world’s biggest and best children’s museum", The Children's...place to reach out to local establishments such as museums, professional organizations, and other nonprofits...CINSO. For instance, I tweeted to the Indiana State Museum and others when I was trying to find local informal...informal science educators, and ever since, the State Museum has been the best supporter and most productive... Children's Museum of Indianapolis. I drove passed these dinosaurs every day. They made me happy. Grad...

  8. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...Paracoccous denitrificans, Pseudomonas spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp....gingivalis, Proteus mirabilis, Providencia spp., Pseudomonas spp., Rhizobium leguminosarum, R. meliloti, Rhodobacter... Rhodobacter sphaeroides, R. capsulatus, Rhodopseudomonas spheroides, Salmonella spp., Serratia marcescens...rettgeri, P. mirabilis, Providencia stuartii, Pseudomonas spp., Rhizobium leguminosarum, R. trifolii, Salmonella...Gluconacetobacter xylinus, Paracoccous denitrificans, Pseudomonas fluorescens, P. putida, Rhizobium meliloti, R...

  9. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...mutations in P. aeruginosa, Pseudomonas putida, Pseudomonas fluorescens, and Pseudomonas syringae. These vectors...system was functional in activating promoters in Pseudomonas syringae and Klebsiella oxytoca. Microbiome engineering...the system as a whole.  Cytidine deaminase for Pseudomonas species Expanding this tool to other organisms...the Ji lab brought genome and base editing to Pseudomonas species (Chen et al., 2018). Using the cytidine...

  10. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...level of translation. RepZ mRNA must fold into a “pseudoknot” held together by intramolecular base pairs for...complementary to part of the sequence forming this pseudoknot, so its binding prevents the mRNA from folding... RepZ is translated from an mRNA that forms a pseudoknot. At high copy numbers, high levels of the lncRNA...lncRNA bind to RepZ mRNA and prevent this pseudoknot from folding, blocking replication. Created with BioRender.com...IncIalpha plasmid ColIb-P9 by competition between pseudoknot formation and antisense RNA binding at a specific...

  11. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...non-native envelopes, a process called pseudotyping. Lentiviruses pseudotyped with the VSV-G envelope protein...and have a broad host range. However, viruses pseudotyped with other envelope proteins may have a limited... "Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors."Nature protocols...Altering the tropism of lentiviral vectors through pseudotyping.” Current Gene Therapy 5.4 (2005). 387-98. Pubmed...

  12. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...and Pol is a polymerase. Envelope gene (may be pseudotyped to alter infectivity): The use of the VSV-G envelope...This system provides the greatest flexibility to pseudotype γ-retrovirus using different envelopes to modify...Gag-Pol and enables additional flexibility in pseudotyping.   Figure 1: Genome of a prototypical...glycoprotein; Broad tropism envelope protein; Pseudotyped to alter infectivity. Packaging Gag Precursor...

  13. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...host. In fact, there is a turn of phrase — “pseudotransduction” — to describe the direct protein transfer...Interestingly, retroviruses are generally only “pseudodiploid” in that — while they have two dimerized linear...C. B., Pacut, C., & Telesnitsky, A. (2008). Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency...two RNA genomes in one retroviral particle: pseudodiploidy and high rate of genetic recombination. Proceedings...

  14. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...capsid and genomic structures. For example, rAAV pseudotypes mix the genome of one AAV serotype with the capsid... capsid of another. Formally, pseudotypes are described with two numbers separated by a slash: the first...capsid. Most vectors provided by Addgene are pseudotypes using the genomic structure of AAV2, so they ...Its genome is the most common choice for AAV pseudotyping. It has broad tissue tropism, including liver...

  15. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...  Boston Museum of Science, where else? Addgenies and other honored guests headed to the Museum of Science...After the speeches, guests were able to roam the museum. I even posed for pictures with some Addgenies....

  16. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...Investigation of multispecies biofilm formed by Pseudomonas spp. and Klebsiella spp. Dr. Devarshi Gajjar,...on a collection of pathogenic Klebsiella and Pseudomonas isolates causing urinary tract infections. This...deletions of important unknown regulatory regions in Pseudomonas and Klebsiella spp. The effects of these deletions...

  17. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...protect the 3′ end, the Liu lab appended RNA pseudoknots, calling this longer RNA an “engineered pegRNA... or epegRNA. Although the addition of the RNA pseudoknot sometimes altered epegRNA expression compared... edits           ~   3′ RNA pseudoknot (epegRNA) Improve pegRNA stability ~ ~ ...

  18. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...and comment on their blogs. 5)   Local science museums are a great place to find opportunities for science...give talks and science cafés at the North Carolina Museum of Natural Sciences and the Morehead Planetarium...

  20. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...broad tropism envelope protein that can also be pseudotyped to alter infectivity. Next, the virus must assemble...when AAV is trafficked. Rep and cap mediate AAV pseudotyping which allows for altered tropism of AAV to target...

  23. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...the starter cell can be achieved by using EnvA-pseudotyped RABV, and only expressing the EnvA receptor (...sequences are supplied during production of EnvA-pseudotyped RABV, these sequences are not copied into viral...

  25. Immunology Research Plasmids and Resources

    Type
    Collection
    ...major histocompatibility complex, class I, H (pseudogene) HLAHP HSP90AA1 heat shock protein 90kDa alpha...IGHV3-11 immunoglobulin heavy variable 3-11 (gene/pseudogene) IGHV311, VH IGHV3-13 immunoglobulin heavy variable...IGHV3D IGHV3-H immunoglobulin heavy variable 3-H pseudogene (provisional) IGHV3H IGHV4-28 immunoglobulin ...immunoglobulin heavy variable 5-A (provisional, gene/pseudogene) IGHV5A IGHV6-1 immunoglobulin heavy variable...IGKV1-13 immunoglobulin kappa variable 1-13 (gene/pseudogene) IGKV113, L18 IGKV1-16 immunoglobulin kappa variable...IGKV1-39 immunoglobulin kappa variable 1-39 (gene/pseudogene) IGKV139, O12, O12a IGKV1-5 immunoglobulin kappa...-15 immunoglobulin kappa variable 3D-15 (gene/pseudogene) IGKV3D15, L16, L16a, L16b, L16c IGKV3D-20 immunoglobulin...

  26. COVID-19 Resources

    Type
    Collection
    ...plasmids, see the table below. SARS-CoV-2 Viral Pseudotyping Collection - This page includes a table of all...are suitable for generating SARS-CoV-2 spike pseudotyped virus. It also lists several luciferase and fluorescent...expression Spike protein constructs for making VSV pseudo virus Mammalian constructs Due to the size and ...

  28. Science as Art

    Type
    Blog Post
    ...collection of Albert G. Richards’ work is held by The Museum of Jurassic Technology in LA, where it is currently...
Showing: 1 - 30 of 70 results