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We narrowed to 311 results for: cas9 genes

Showing: 1 - 30 of 311 results

  1. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...Figure 2: Cas9 expression in monoclonal cell lines generated from A549 cells transduced with lentiCas9-Blast...A549 cells stably expressing Cas9. A549 cells were transduced with lentiCas9-Blast 1 and then selected with... express Cas9 and are included as a control (WT). Vinculin is a loading control. 1 lentiCas9-Blast was...additional 3 weeks under blasticidin selection. Anti-Cas9 Western blotting was performed on whole-cell extracts...Blog Posts Addgene Protocols Viral Service Introduction This protocol describes how to generate a monoclonal...Selective pressure on this heterogeneous cell pool could lead to reduced transgene expression over time, as...more likely to retain stable transgene expression. Other methods of generating a monoclonal cell line include...

  2. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...constantly improving. Cas9 variants have been modified for genome editing, activating gene expression, visualizing...off-target effects” have included using a Cas9 nickase variant, lowering Cas9 expression, and truncating gRNA sequences... target effects of CRISPR/Cas9 by altering the nuclease activity of the Cas9 nuclease itself. Design and...non-target strand and Cas9, Kleinstiver et al disrupted interactions between Cas9 and the phophate backbone...specificity of the Cas9 nuclease with engineered variants: eSpCas9, SpCas9-HF1, & HypaCas9. The off-target...portions of Cas9 might be important for nuclease specificity. They found that eSpCas9(1.1) and SpCas9-HF1 each...from this publication at Addgene. Resources on the Addgene Blog Read about Cas9 Homolog Cpf1 Read How ...

  3. Components of CRISPR/Cas9

    Type
    Blog Post
    ...sequence, Cas9 will neither bind nor cut the target. Cas9 homologs from different organisms or Cas9 mutants...basic level, CRISPR/Cas9 genome editing systems use a non-specific endonuclease (Cas9 or closely related...the PAM sequence. Cas9 Species/Variants and PAM Sequences Species/Variant of Cas9 PAM Sequence ...genomic target for Cas9, and the tracrRNA which acts as a scaffold linking the crRNA to Cas9 and facilitates... to direct Cas9 to a specific genomic locus and the scaffolding sequence necessary for Cas9 binding. When...CRISPR/Cas9 for genome editing, researchers simply need to express a gRNA designed to direct Cas9 to their...tracrRNA and Cas9, bind to these transcripts and C) cleave them leaving mature crRNAs bound to the Cas9/tracrRNA...

  4. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...Figure 3: Gene repression with nuclease-competent Cas9. When complexed with a truncated gRNA, Cas9 is still... site (TSS) of a gene of interest, it induces potent gene expression with no genetic alterations observed...efficiently transcribing the gene. This results in gene silencing, with no observed genetic alterations at the ... gRNA, Cas9 binds the target site, but does not cut either strand of DNA. Traditional Cas9 regulators... modifications to DNA, Cas9 can be reprogrammed to serve as a regulator of gene expression. Its catalytic...promoter of a gene of interest, they have been shown to dramatically alter levels of expression. Cas9’s ability... of modulating gene expression makes it an invaluable tool in the interrogation of gene function. However...

  5. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...including: generating single or multi-gene knockouts in a wide variety of cell types, gene editing using... can occur due to prolonged Cas9 expression     The requirement for Cas9 transcription and translation...consisting of the Cas9 protein in complex with a targeting gRNA, to your cells of interest. Cas9 RNPs are capable...pathways. This makes Cas9 RNPs useful for CRISPR applications where limited expression of Cas9 is required and...express and purify His-tagged Cas9 from E. coli using a plasmid such as pET-28b-Cas9-His from Alex Schier’s ... of Cas9-gRNA RNPs by specific cell types (Rouet et al. 2018). This was accomplished using a Cas9 mutant...for experiments where stable Cas9 expression is required, such as when a Cas9 fusion to a fluorescent protein...

  6. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ... Split Cas9, Split Cas9 nickase, Split dCas9 Genome editing by NHEJ and HDR, repression of gene expression...Photoswitchable Cas9 Richter et al., 2016 tsRC9 RsLOV from R. sphaeroides Cas9, dCas9 Gene editing Zhou...oligonucleotides Cas9, photocaged-gRNA Gene editing Manna et al., 2019   NPPOC-caged trimethoprim (TMP) Cas9 fused...domains (Cas9-DHFR) Gene editing, transcription Zhou et al., 2020   NPOM-caged nucleobases Cas9, photocaged-gRNA...showing that this genetically encoded, photocaged Cas9 system could silence endogenous gene expression - demonstrating...Zhou et al., 2018   pdDronpa Cas9, dCas9 Gene editing, transcription Light-Dependent Anti-CRISPR Bubeck...-CRISPR protein, co-expressed with Cas9 or dCas9 Repression of gene editing and transcription. Label genomic...

  7. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...frequently used Cas9, spCas9 from Streptococcus pyogenes, this sequence is 3’-NGG. But Cas9s found in other...Lab used directed evolution to generate Sniper-Cas9 resulting in a Cas9 with improved specificity. They...assessment of the off-target activity of Cas9. This can occur when Cas9 cleaves a site that is not a perfect...the PAM specificity of Cas9s isolated from different organisms and engineered Cas9 variants that were able...consideration when choosing a Cas9 is balancing specificity and sensitivity. Wild-type Cas9 exhibits high on-target...on-site and off-site activity of Sniper-Cas9 to different Cas9 variants using gRNAs of different lengths...loci and gRNA lengths. Recent alternatives to Cas9 While Cas9 remains the most widely used CRISPR nuclease...

  8. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...introducing Cas9-mediated frameshift mutations in the gene of interest. The genomic target for Cas9 and the...using a Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9 for site-specific...target genes. Repressing target genes using dCas9-based repressors Early experiments using dCas9 in bacteria...frequently fused to Cas9 for this purpose. dCas9-KRAB plasmids for repressing target genes in a variety of...Next CRISPR Experiment Cas9 expression systems Addgene carries a variety of Cas9 containing plasmids that... CRISPR/Cas9 has made it easier than ever to make precise, targeted genome modifications. Cas9 has been... repress, and even image your favorite gene. With so many Cas9 reagents available, it can be difficult...

  9. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ... context of the gene, Cas9 activators efficiently solve these problems.   Which Cas9 activator should...prevent your gene from being up-regulated. For instance, generally, the more highly expressed a gene is under...with CRISPR/Cas9 Learn How CRISPR Has Been Used with AAV Resources on Addgene.org Find Cas9 Activators...CRISPR/Cas9 is an enormously plastic tool and has taken the scientific world by storm. While Cas9 has been...the upregulation of essentially any gene by fusing mutants of Cas9 deficient in DNA cutting activity to... research CRISPRa for specific gene targets CRISPR activation for genes with known functions has the potential.... Your gene of interest might already be hitting an upper bound of activation that current Cas9 systems...

  10. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...CRISPR/Cas9 genome editing varies across targets, they tested two genes in mice. For both genes, they ...one AAV, Cas9 must be made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9 (~3.3 ...amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is the smallest Cas9 ortholog characterized...used CjCas9 with AAV to target genes in mouse muscle and eye tissue. Neisseria meningitidis Cas9 (NmeCas9...editing, but in vivo gene editing approaches have been limited by problems with Cas9 delivery. Adeno-associated...vehicle for Cas9. However, to allow for more room for regulatory sequences while still fitting Cas9 and its...equally potent Cas9 protein, the Zhang lab took a different approach. They analyzed over 600 Cas9 orthologs...

  11. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...the basic parts of the Cas9 enzyme.   Figure 1: A cartoon depiction of Cas9’s two major lobes, REC...   Unbound Cas9 Now that we know what we’re looking at, let’s peek at unbound—“apo”—Cas9 from Streptococcus...on these regions.   Cas9’s transition from weak, random binding to precision gene editing requires three...Figure 8: Comparison of Cas9:RNA crystal structure (left, PDB ID 4ZT0) with Cas9:RNA:DNA electron microscopy...Streptococcus pyogenes.   Figure 2:  Crystal structure of S. pyogenes Cas9 in the apo state (PDB ID...   Figure 3: Crystal structure of S. pyogenes Cas9 bound to guide RNA (PDB ID 4ZT0), shown opaque...isn’t in place yet.     Figure 4: S. pyogenes Cas9 in the apo state (PDB ID 4CMP, top) compared...

  12. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...out to make my first CRISPR/Cas9 gene edit. I wanted to inactivate the gene BRAF (a kinase implicated in...virally-delivered GFP gene. So, cells with no Cas9 should appear green and cells with functional Cas9 should appear...analyzing a gene edit using Addgene’s lentiviral CRISPR tools. Schematic overview of CRISPR/Cas9 gene...experiments, we generated A549 cells stably expressing Cas9 via transduction with Cas9 lentiviral vector... Figure 1: Cas9 expression in A549 cells transduced with lentiCas9-blast. Anti-Cas9 Western blotting... Cas9 delivery Overview: Before any editing can begin we need our A549 cells to express Cas9. In these...with too many Cas9 integrations per cell, and my stable cell pool had wildly variable Cas9 expression (...

  13. Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

    Type
    Blog Post
    ...They went on to show that their CRISPR/Cas9-nuclease and CRISPR/Cas9-nickase vectors work successfully to...versions of Cas9, nuclease and nickase, but we are now adopting dCas9, dCas9-VP64, and dCas9-KRAB in our...24954249 Find the Mulitplex CRISPR/Cas9 Assembly System Kit at Addgene here. Other Popular CRISPR Posts...all-in-one vector construction system for CRISPR/Cas9-mediated multiplex genome engineering is now available...one of the most advantageous properties of CRISPR/Cas9 compared to ZFNs and TALENs,” said Tetsushi Sakuma...offers ease and efficiency for all-in-one CRISPR/Cas9 vectors expressing up to seven guide RNAs (gRNAs...Figure 1: Schematic overview of the all-in-one CRISPR/Cas9 vector construction system for multiplex genome ...

  14. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...use of novel S. pyogenes Cas9 variants with varying PAM sequences, 2) the use of Cas9 homologs derived...as a binding signal for Cas9 and this sequence is a strict requirement for Cas9-mediated DNA cleavage. ...when trying to edit a gene using homology directed repair, since HDR-mediated gene editing is most efficient...2020). Characterization of Cas9 from additional bacterial species Many more Cas9 orthologs have been isolated...them more useful than SpCas9. For example, Cas9 from Staphylococcus aureus (SaCas9) is about 1 kilobase... in length, Cas9 from Campylobacter jejuni (CjCas9) is even smaller than SaCas9 and is also compatible...While PAM sequences for the commonly used S. pyogenes Cas9 (3'-NGG) are abundant throughout the human genome...

  15. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...Postdoctoral Researcher in Jacob Corn’s lab. CRISPR-Cas9 (Cas9) is an RNA-guided nuclease that targets and cuts...outcomes: Cas9 breaks repaired by the non-homologous end-joining (NHEJ) pathway disrupt target gene sequences...replacement by HDR. Understanding Cas9 biophysics enhances HDR We first measured Cas9 dissociation rates and discovered...error-prone repair of the gene would be disastrous (i.e. if breaking the gene is worse than leaving the...(in our hands) this technique supports gene disruption or gene replacement frequencies that are an order...interaction with the Cas9-DNA duplex. Image from Richardson et al., 2016.   dCas9 enables sequence replacement...normally accompanies Cas9 cutting. We still don’t know the mechanism underlying dCas9 editing, but it could...

  16. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...Pten by CRISPR/Cas9 in Mouse Liver in Spite of Cas9-Specific Immune Responses. Human Gene Therapy 26:432...Schematic of targeted gene knock-out (left) and targeted DNA knock-in (right) by CRISPR/Cas9. Adenoviral CRISPR...vectors. This capacity is large enough to carry the Cas9 gene and a gRNA expression cassette in one single viral...targeted organs or tissues with Cas9 and a gRNA designed to target a mutated gene. In a Scientific Reports paper...chromosomal region. The CRISPR/Cas9-derived RNA-guided nuclease-induced gene disruption frequencies in the...DNA Adenoviral CRISPR/Cas9 genome editing tools can also be used to deliver Cas9 and DNA templates in ... CRISPR/Cas9 research! To find more information about the adenoviral delivery of CRISPRS/Cas9 using the...

  17. Keeping Up With CRISPR/Cas9

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    Blog Post
    ...more References: Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell...CRISPR/Cas9 system to precisely edit specific genes in monkeys. "Our study shows that the CRISPR/Cas9 system...for CRISPR Plasmids? CRISPR-Cas9 Plasmids available at Addgene CRISPR-Cas9 References, Protocols, and ...advances in the understanding and application of CRISPR/Cas9 technology are coming along at a fast and furious...system enables simultaneous disruption of two target genes in one step without producing off-target mutations...Beisel and Rodolphe Barrangou, had applied the CRISPR/Cas9 system to developing an antibiotic that selectively...important details in their understanding of how CRISPR/Cas9s actually work. Last month, the Lawrence Berkeley...

  18. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post
    ...a cell. ZFNs and CRISPR/Cas9 have also been used to create mutations in genes essential to HIV-1 replication...or apoptosis with Cas9/gRNA expression. A pooled analysis found no evidence of Cas9 cleavage at predicted...escape from CRISPR/Cas9-induced modification targeting either the LTRs or essential genes. Many of these ...were located near the Cas9 cut site, leading Wang et al. to conclude that some Cas9-derived indels may not...Genomes from Human T-lymphoid Cells by CRISPR/Cas9 Gene Editing. Sci Rep. 2016 Mar 4;6:22555. PubMed PMID...to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from Addgene. This system uses...CRISPR/Cas9 escape occurs in an animal model. If these escape mutations are frequent, the dCas9-mediated...

  19. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...needed by Cas9 for cleavage. In certain cases, Cpf1 may be better suited for genome editing than Cas9 - read...CRISPR system. Like Cas9, Cpf1 contains a RuvC-like endonuclease domain, but it lacks Cas9’s other HNH endonuclease...Potential advantages of Cpf1 over Cas9 Type II CRISPR systems based on Cas9 were thought to be the simplest...directional gene transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer...than that of the widely used SpCas9 and comparable to SaCas9. As seen with Cas9 orthologs, Cpf1 efficiency...rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins...Again, they were in for a surprise! Whereas Cas9 generates blunt ends 3 nt upstream of the PAM site, Cpf1...

  20. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...several Cas9s that recognize NG PAMs. The Nureki lab generated SpCas9-NG, a rationally engineered Cas9 with...PAM-interacting domain of Cas9. After multiple rounds of engineering, they developed the SpRY Cas9 variant (Walton... at NGH PAMs (Zhang et al, 2021) NG Cas9 NG - SpG Cas9  NG  - SpRY nuclease  C to T Base Editor...notes SpCas9 NGG - Cas12a TTTV Great for AT-rich regions Cas9 EQR NGAG - xCas9 NG Less...of gRNA placement significantly over SpCas9 and more so than Cas9s with an NG PAM.   Cas PAM sequence...    Fig. 1: SpCas9 has sparse PAM coverage in the human genome. SpRY Cas9 has PAM sites covering...blog The PAM Requirement and Expanding Beyond Cas9 xCas9: Engineering a CRISPR Variant with PAM Flexibility...

  21. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ... encoding the gRNAs and Cas9. As with the Gersbach lab plasmids, multiple Cas9 variants are available:...subsequently inserted into a Cas9-containing destination vector based on pCAMBIA. Cas9 is plant-optimized, and...humanized Cas9, D10A nickase mutant (Cas9n), dCas9 (transcriptional repression), and Fok1-dCas9 (dimeric...options include humanized wt Cas9, dCas9 (transcriptional repressor), and dCas9-VP64 (transcriptional activator... then digested using BsmBI and ligated into Cas9 or dCas9-containing destination vectors. Destination ...the multiple gRNAs and Cas9 together into a single plasmid. Although only wt hCas9 entry vectors are supplied...plasmids from this paper at Addgene. 2. Kabadi, Ami M., et al. “Multiplex CRISPR/Cas9-based genome engineering...

  22. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post
    ...or see Addgene's CRISPR guide page for a review of Cas9.  For a brief comparison of Cpf1 vs. Cas9, see ...Comparing the Cas9 and Cpf1 CRISPR Nucleases   Cas9 Cpf1 Components crRNAtracrRNACas9 crRNACpf1 ...method, other multiplex CRISPR gene editing methods relied solely on Cas9. Overall, these approaches have...promoters).  spCas9 and its gRNAs are also larger than their Cpf1 counterparts. Table 2. Cas9 Multiplexing...Resources on the Addgene Blog Cpf1: A New Tool for CRISPR Genome Editing Which Cas9 Do I Choose for My... A Match Made in Heaven: CRISPR/Cas9 and AAV Resources on Addgene.org Browse Our CRISPR Multiplexing... system for editing multiple targets compared to Cas9.  Read on to learn more about Cpf1 multiplexing....

  23. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    Blog Post
    ...stably integrate a reporter gene that contains a puromycin resistance gene followed by the target site...contains Cas9. The Madison lab also created an inducible SRIRACCHA system (iSRIRACCHA) where Cas9 expression...that targets your desired gene (whose sequence is also found in the reporter gene). A control RFP expression...while cells containing the reporter gene that was cleaved by Cas9 and repaired via HDR using the supplied...plasmids at Addgene GEMCherry2, an improved monomeric fluorescent protein for assessing CRISPR/Cas9 activity... labor intensive and quite time consuming.   As Cas9/gRNA activity is crucial for successful CRISPR editing...the scientific community for efficient real-time Cas9 activity assays. Recently scientists have created...

  24. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post
    ...similarities and differences to Cas9. DNA targeting Cas enzymes Cas9 – The genome engineering workhorse...CRISPR’ without shortly thereafter hearing ‘Cas9’. The Cas9 nuclease is a genome engineering powerhouse...making Cas9 an adaptable choice for many genome engineering endeavors. For more information on Cas9 variants...genome where it will proceed to generate a blunt DNA double strand break. Cas9 has been studied for genome...Cpf1, Cas12’s primary difference from Cas9 is that it generates staggered breaks, also known as sticky...same mechanism as traditional spCas9. If you are looking to modify RNA, Cas9 just won’t cut it – literally..., unlike Cas9, it targets RNA instead of DNA. This makes it particularly useful in generating transient...

  25. Top Requested Lentivirus and AAV of 2016

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    Blog Post
    ...express Cas9 and a gRNA from the same plasmid. LentiCas9-blast virus can be used to generate Cas9-expressing...this plasmid Cas9 expression is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses...While various Cas9 mutants have been engineered to perform unique editing tasks, classic Cas9 nuclease, which...libraries) and individual gene knockouts (using gRNA lentiviruses). Separating Cas9 and the gRNA into two ...gRNAs in a monoclonal Cas9 background because you’ll have more consistent levels of Cas9 expression between... expressed from lentiCas9-Blast. Purpose of LentiCas9-blast LentiCas9-blast is a Cas9 expression vector...growth rate variability when using lentiCas9-blast to generate Cas9-expressing cells. To ensure that this...

  26. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...have created Cas9 enzymes with looser PAM requirements, which are available from Addgene!   RNA crRNA:..., simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the... Sheet  Resources on the Addgene blog A Needle in a Base-Stack: Cas9 Structural Biology...elegant bacterial immune system and an efficient gene editing tool… but boy does it have a lot of parts...chromosome encodes a tracrRNA (in some systems including Cas9), Cas proteins, and a CRISPR array. The CRISPR array...bacteria, these crRNAs are bound by Cas proteins (Cas9 shown here). The repeat sequence base pairs with...“single-guide RNA” that performs both functions. Cas9 cuts both the target and nontarget DNA strands upstream...

  27. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...Publication at Addgene Cas9 and NgAgo head to head NgAgo has a number of unique features compared to Cas9. These...Resources on the Addgene Blog Read About Cas9 Homolog Cpf1 Check Out High Fidelty Cas9 Mutants Browse ...lines, with an efficiency similar to that of Cas9. Like Cas9, NgAgo is also able to mediate homology-directed... certain guide sequences - using 5 guides/gene for 10 genes, they did not observe differences in cleavage... BELOW Biologists are going gaga over the newest gene-editing protein - a DNA-cleaving Argonaute from ... why you might choose NgAgo over CRISPR proteins Cas9 or Cpf1 - keep reading to learn more! Background...These include: More targeting flexibility Unlike Cas9 and the other CRISPR enzymes, NgAgo does not require...

  28. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    Blog Post
    ...researching a way to treat AD using CRISPR/Cas9. They are targeting the genes responsible for both early onset ...mutation in a gene that causes cataracts could be rescued by coinjection into zygotes of Cas9 mRNA and a ...5. Rohn et al. 2018. “The Potential of CRISPR/Cas9 Gene Editing as a Treatment Strategy for Alzheimer’...Lucie is an Addgene co-op from Northeastern University.  There can be no doubt that CRISPR/Cas9 technology...applications.” Li’s team noted that CRISPR/Cas9 has been used to generate mutations in a wide range of organisms...glance at genome editing with CRISPR-Cas9 technology.” Current genetics. https://doi.org/10.1007/s00294-019...2020. . “Advances in CRISPR/Cas-based Gene Therapy in Human Genetic Diseases.” Theranostics. https://doi.org...

  29. Genome-wide Screening Using CRISPR

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    Blog Post
    ...trying to target every gene in the genome, or a specific class of genes? Addgene currently carries several...versatility. CRISPR/Cas9 consists of two components: the non-specific endonuclease (Cas9) and a single stranded...within the target gene. CRISPR can also be used to activate or repress target genes without permanently... of a CRISPR screen is to use Cas9 and a pool of gRNAs to identify genes that are essential for a given...repress target genes by combining a gRNA library with the aforementioned derivatives of Cas9. Several CRISPR... in a different gene. Libraries may be available in a 1-plasmid system, in which Cas9 is included on the... gRNAs targeting the same gene can be strong evidence that a particular gene is actually important for...

  30. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...dual-nuclease Cas9-Cas9 chimeras Scot Wolfe’s lab has developed dual-nuclease Cas9-Cas9 chimeras with ...compared to monomeric Cas9’s. Read the paper in Nature Communications Find the Cas9-Cas9 chimera plasmids...proteins for optogenetic control of CRISPR-Cas9. By combining AcrIIA4, a Streptococcus pyogenes Cas9 inhibitor...plasmids Anti-CRISPR proteins for optogenetic control of CRISPR-Cas9 Anti-CRISPR proteins that inhibit... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...adapted for detecting SNPs. Learn more from this Addgene blog post Read the paper in Science Find the...editing is now possible. Learn more from this Addgene blog post Read the paper in Nature Methods Find...
Showing: 1 - 30 of 311 results