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We narrowed to 91 results for: tev

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Showing: 1 - 40 of 91 results

  1. Hot Plasmids - August 2020

    Type
    Blog Post
    ...of the TEV cleavage and improve utility of the system for use with low affinity variants of TEV. The new...evolution platform to generate TEV protease variants. Catalysis by TEV protease allows a transcription...catalytic efficiency of tobacco etch virus protease (TEV), a highly sequence-specific protease used to cleave...

  2. Plasmids 101: Protein tags

    Type
    Blog Post
    ...best optimized is the tobacco etch virus (TEV) protease. A TEV protease cleavage site is frequently placed...comprised of a calmodulin binding peptide (CBP), a TEV cleavage site (more on that in a moment), and 2 ProtA...cleavage reaction taking place between column runs. The TEV protease itself, with various mutations used to increase...with tags Protease Recognition site Notes TEV ENLYFQS Cleaves between the Gln and Ser residues ...

  3. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...Hexahistidine tag with TEV cleavage, AmpR 26093 pET15-MHL EF456738 Hexahistidine tag with TEV cleavage, AmpR ...Hexahistidine tag with TEV cleavage, KanR 26105 pNIC-CTHF EF199844 Hexahistidine tag, FLAG tag with TEV cleavage, ...thioredoxin with TEV cleavage, KanR 26107 pNIC-ZB GU452710 Hexahistidine tag , Z-basic, TEV cleavage, AmpR... 26096 pET28-MHL EF456735 Hexahistidine tag with TEV cleavage, KanR 26098 pCW-LIC EF460848 No tag, double...26108 pFB-LIC-Bse EF199842 Hexahistidine tag with TEV cleavage, AmpR 26095 pFHMSP-LIC-N N/A Expression ...secreted proteins with N-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac Expression System, AmpR 26100...secreted proteins with C-terminal hexahistidine tag and TEV cleavage, Bac-to-Bac Expression System, AmpR Related...

  4. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...few of our proteins that lacked TEV. Although we were unable to cleave TEV within the protein we tested,...the AviTag. As a side note, we found that adding a TEV linker dramatically increased accessibility of the...

  5. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Gitler 29629 GST-TEV-FUS FUS TEV, GST T7 ALS Aaron Gitler 29630 GST-TEV-FUS-267-526aa FUS TEV, GST T7 ALS ...29631 GST-TEV-FUS-1-373aa FUS TEV, GST T7 ALS Aaron Gitler 29632 GST-TEV-FUS-1-413aa FUS TEV, GST T7 ALS...pGST2-GST-TEV-OPTN S177D S473D OPTN GST-TEV ALS Sascha Martens 187830 pFastBac_Dual_GST-TEV-EGFP-TBK1 ...pET-Duet1-His-TEV-mCherry-OPTN WT OPTN His, TEV, mCherry ALS Sascha Martens 190192 pET-Duet1-6xHis-TEV-OPTN OPTN...-GST-TEV-mCherry-TBK1 TBK1 mCherry, GST, Tev P10 ALS Sascha Martens 198037 pFastBac_Dual_GST-TEV-EGFP-...666) TBK1 GFP, GST, Tev P10 ALS Sascha Martens 198428 pGST2-GST-TEV-OPTN OPTN TEV, GST tac ALS Sascha ...PRKCG PRKCG His, TEV T7 Spinocerebellar ataxia 18 Nicola Burgess-Brown 39042 GAK GAK TEV, His T7 Parkinson's...

  6. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...mTagBFP2 tag pNIC-GST-TEV-GG - Cloning of target gene with N-terminal GST tag and TEV cleavage Transient ...mammalian expression pET His6 GST TEV - N-terminal His6-GST-TEV for bacterial ...Protein purification pET28-MBP-TEV - Bacterial expression with MBP-TEV N-terminal fusions pDEST Gateway...with N-terminal His-TrxA-TEV tag NusA Protein purification pNIC-HIS6-NUSA-TEV-GG - Cloning of target gene... His6 NUSA tag and TEV cleavage pExp-NusA - Expression of protein in E. coli as TEV cleavable N-terminal...expression pNIC-CTHF - C-terminal TEV-His6-Flag for bacterial expression pFA6a vectors ...

  9. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...MitoTRACER system. NLS = nuclear localization signal; TEVp = TEV protease. Created with BioRender.com. Using...cells express iCre with a mitochondrial anchor and a TEV cleavage site. Recipient cells express DsRed within...lox-STOP-lox sequence, followed by the EGFP, and the TEV protease. Once the recipient cells take up the Cre-tagged...

  11. Open Enzyme Collection

    Type
    Collection
    ...Addgene ID Plasmid Gene/insert 165509 pOpen-TEV protease TEV protease 165565 pOpen-RNAse A RNAse A 165575...

  17. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...C-Halo , pCS2+/N-Halo - Xenopus Expression pET51b-His-TEV-HaloTag7 - Bacterial Expression SNAP-Tag Benzylguanine...CLIPf (w877-2) - Gateway Entry Vector pET51b-His-TEV-CLIP-tag - Bacterial Expression Return to top Additional...

  18. Genetic Code Expansion

    Type
    Collection
    ...228787 pAcBac3-OMeYRS-8xYtR-EGFP-3TAA-40TGA-152TAG-TEV-3xUAA OMeYRS E. coli O-methyltyrosine (OMeY) Mammalian...228788 pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA pAAFRS E. coli p-aminophenylalanine (pAAF) ...

  19. Bacterial Expression Systems

    Type
    Collection
    ...tags: MBP, SUMO, TrxA, Mocr, NusA Cleavage sites: TEV protease, factor Xa, enterokinase, thrombin Signal...

  20. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ... knock-out in your experiments? AAV-flex-taCasp3-TEVp from Nirao Shah's and Jim Well's lab induces cre-dependent...viral prep in serotype AAV5. Find AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1) targeting...

  21. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...and Chemistry activities from Quatr.us Foldscopes Steve Spangler Science The Fab Lab Middle school: supplementing...exciting, tumultuous time in scientific history. Whatever your reasons, let us know how it goes! Acknowledgements...

  22. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...are then washed, followed by antibody elution. Whatever method you choose, you may not be able to elute...time, reagents, and equipment available to you. Whatever you choose, you’ll want an accurate concentration...

  23. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...the transgenic genome will simply express from whatever promoters were included in the vector (or are ...instead get expression of the sensor, marker, or whatever other tool you chose to express. In the case ...

  24. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...there. Make sure you read the instructions for whatever antibiotic you choose to ensure it works in your...molecular language as the tree outside my window! Whatever you use them for, shuttle vectors can help you...

  25. How to Write a Scientific Review Article

    Type
    Blog Post
    ..., you can easily convert the citation style to whatever the journal requires. Using the (Last name, Year...woods for months at a time, so take advantage of whatever time you have. If you’re like me, you might be...

  28. AAV Molecular Tools

    Type
    Collection
    ...System Activity Serotype PI 45580 pAAV-flex-taCasp3-TEVp EF1a-driven, Cre-dependent Cre-dependent, bicistronic...bicistronic expression of designer pro-taCasp3 and TEVp for studying cell ablation. 1, 5, 8 Nirao Shah , Jim...Jessell , Eiman Azim 207997 pAAV hSyn-fDIO-taCasp3-TEVp Syn-driven, Cre-dependent Inducing apoptotic ablation...GFP (cfGFP) under synapsin promoter. 8 Alexander Dityatev 200778 AAV-∆Hapln1 hSyn-driven, constitutive Expresses...GFP (cfGFP) under synapsin promoter. 8 Alexander Dityatev 203840 pAAV-hSyn-Kir2.1WT-2A-mScarlet-KASH hSyn-driven...

  29. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...Meltzer, J. C., Buzhdygan, T., Cheng, M. J., Sena-Esteves, M., Bennett, R. E., Sullivan, T. P., Razmpour,..., Allaway, K., Sakopoulos, S., Schneider, G., Stevenson, O., Vergara, J., Sharma, J., Zhang, Q., Franken...

  32. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...electronically or choose to auto-approve MTAs. Whatever your preference, we are here to make it fast and...
Showing: 1 - 40 of 91 results