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We narrowed to 73 results for: retroviral vector

Showing: 1 - 50 of 73 results

  1. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...using retroviral vectors in your experiments. Gamma-retroviral vectors Popular gamma-Retroviral Transfer...experiments. Gamma-retroviral vs. lentiviral vector systems Gamma-retroviral vectors have a relatively ...For research purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating...and expression of these vectors when used in experiments. Retroviral vectors make up a large toolbox ...Transfer Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning...building block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial...enable long-term stable gene expression. Gamma-retroviral vectors are derived from the Moloney Murine Leukemia...

  2. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    ...Addgene blog Viral Vector Elements The Retroviral Life Cycle Popular Retroviral Vectors and Their Use in...prototypical gamma-retrovirus.    Frequently asked questions about γ-retroviral vectors How do engineered...methods, the following components are needed: γ-Retroviral transfer plasmid encoding a transgene, sgRNA,...viral LTRs, differ. As a result, lentiviral and γ-retroviral packaging plasmids are not interchangeable. General...lentiviruses may not be efficiently packaged by γ-retroviral packaging systems, and vice versa. Envelope plasmids...lentiviruses, the genes required for γ-retroviral packaging and transduction are not encoded by the transfer...longer present in these constructs. Why do many γ-retroviral plasmids have similar names? The nomenclature...

  3. Viral Vectors 101: The Retroviral Lifecycle

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    ...blog Viral Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors 101: Viral...foundation for producing and using retroviral vectors effectively. Retroviral structure   Figure 2:...have been developed into retroviral vectors and 2.) a subclass of retroviruses called the lentiviruses (...deep understanding of retroviral biology is necessary in order to use the vectors correctly. A solid understanding...structure of a retroviral particle, which is essentially the same for viruses and viral vectors. Key to the...would not be great for a gene therapy vector! For viral vectors, only the protein for reverse transcriptase...components of the vector particle, some of the RT protein gets packaged inside of the vector particle, along...

  4. Plasmids 101: Multicistronic Vectors

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    ...MSCV-IRES-EGFP IRES Retroviral pMSCV-pBabeMCS-IRES-RFP IRES Retroviral pMSCV-IRES-YFP II IRES Retroviral pCMMP-MCS-IRES-Puro...pCMMP-MCS-IRES-Puro IRES Retroviral pEF1a-IRES-Neo IRES Mammalian MSCV-IRES-Luciferase IRES Retroviral pWPI IRES... the creation of bicistronic or multicistronic vectors. Unlike promoters which will create unique mRNA...for each gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins...blog post we’ll cover basics of multicistronic vectors: why they are useful, how they work, and how to...get started with them. Why Use multicistronic vectors? Detecting cells that are expressing your gene,...expressing your gene of interest at high levels. Unlike vectors that express screenable or selectable markers from...

  5. Your Lentiviral Plasmid FAQs Answered

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    ...ingredients: the lentiviral packaging vector, a transfer vector including the gene of interest, and the...different. As a result, lentiviral and retroviral packaging vectors are not interchangeable. While these...3rd-generation transfer vectors apart? A3: Addgene defines 2nd- or 3rd-generation transfer vectors based on whether...between 2nd- and 3rd-generation vectors? What does this mean for transfer vectors? A4: The lentiviral packaging...largely based upon the vector system used and the transgene insert encoded by the vector." The table below ...Q1: Are retroviruses and lentiviruses the same thing? Can I package lentivirus with retroviral packaging...Lentiviruses are part of the retroviral family. What scientists often refer to as “retroviruses” are technically ...

  6. Viral Vectors 101: Viral Vector Elements

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    ...of viral vectors here! Need Virus? Check out Addgene's Viral Service! Types of viral vectors Of the many...types, but most vectors are based on adenovirus 5 (Ad5).     Fig. 3 - Viral vector components of...of adenovirus Building viral vectors Addgene has many viral vectors and helper plasmids for all the...Harbor Laboratory Press; 1997. Principles of Retroviral Vector Design. Available from: https://www.ncbi.nlm.nih.gov...breakdown the vector components first by virus and then by function. Lentivirus/gamma-retrovirus Lentiviruses...these vectors that aren’t reflected in the common components outlined above for gamma-retroviruses. While...components.     Fig. 1 - Viral vector components of retrovirus  Adeno-associated virus AAV’s elements...

  7. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    ...lentiviral vectors, how they work, and why you might choose them over integrating lentiviral vectors.  Before...integrase-deficient lentiviral vectors, let’s briefly revisit the basic biology of lentiviral vectors (for a more detailed...lentiviral vectors that are competent for one round of infection (AKA transduction in the viral vector world...life cycle of a lentiviral vector with an integrase-deficient viral vector. Created with BioRender.com...lentiviral vectors? There are several reasons why you might choose an IDLV over a lentiviral vector: 1. No...include detecting vector particle components or performing quantitative PCR titration of vector DNA in samples...resources on the Addgene blog Viral Vectors 101: The Retroviral Life Cycle Quick Guide to All Things ...

  8. Quick Guide to All Things Lentivirus

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    ...fluorescent sensors. Retroviral vector background Until the development of viral vectors, gene transfer into...un-transfectable cells, labs use viral vectors and particularly retroviral vectors because they target a broader...choice for a gene transfer vector. Lentiviral vectors Although retroviruses are still in use, considerable...your experiment using the lentiviral vector system. Viral vectors have been increasingly popular in fundamental...primary cells. Amongst the different vectors used, lentiviral vector constructs have proven very useful ... the genome is more efficient. The first retroviral vectors used were derived from the Moloney Murine...generation of lentiviral vectors consisted of three plasmids encoding 1) the lentiviral vector genome which was...

  9. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    ...pressure on the production of retroviral vectors: Enhancement in vector stability." Biotechnology and ...guide to viral vectors Find all of our viral vector blog posts Download our viral vectors 101 eBook Additional...been actively investigating ways to improve viral vector production. Whether redesigning viral packaging...membrane When packaging adeno-associated viral vectors (AAV) not all serotypes are equal; while some serotypes...increased yields in adenoviral (Coroadinha, 2006) and retroviral production systems (Shen, 2011). To our knowledge...we believe that this approach can be adapted by vector cores and laboratories alike as an easy and inexpensive...Efficient serotype-dependent release of functional vector into the culture medium during adeno-associated...

  11. Plasmids 101: Common Lab E. coli Strains

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    ...cloning into and storage of lentiviral and retroviral vectors or cloning or repeated sequences with the...and storage of unstable DNA and lentiviral/retroviral vectors. Used by Addgene for pooled library amplification...Replication The Promoter Region – Let's Go! Mammalian Vectors Looking for More Plasmids 101 Articles?  Find ...

  12. Delivery Methods for Generating iPSCs

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    ...murine leukemia virus (MMLV)-derived retroviruses. Retroviral vectors, such as pMXs or pMSCV, infect dividing...safety required for translational use. Viral Vectors Retroviral: Some of the first iPSCs were generated ...corresponding endogenous pluripotency genes. Retroviral vectors are prone to incomplete silencing of reprogramming...cloning capacity (8-10 kb) than other retroviral vectors. This makes it possible to use a polycistronic...reprogramming vectors available from Addgene. Another drawback is that lentiviral vectors insert in different...Cre-lox lentiviral vectors: This approach is the same as using a standard lentiviral vector, but transient...Non-integrating iPSC delivery methods Viral Vectors Adenovirus: Adenoviral vectors infect dividing and nondividing...

  13. 15 Years of Addgene: The Top 15 Plasmids

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    ...  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab is...opening our UK office in 2014, to starting our viral vector service in 2016...we’re excited to help scientists... be used with 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such as our...lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the Zhang lab is used to deliver Cas9...your gRNA of interest into mammalian cells. This vector was the top requested plasmid of 2015. What makes... This plasmid from the Zhang lab is a mammalian vector that expresses both SpCas9 and your gRNAs and was...envelope protein for producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids...

  14. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...generated a series of retroviral vectors expressing tagged dCas9 and guide RNA. The retroviral enChIP system ...requested plasmids for enChIP through Addgene. Retroviral Expression System for enChIP using CRISPR Addgene...Addgene: Your lab recently developed a retroviral enChIP system using CRISPR technology. Can you describe...Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for enChIP Using CRISPR. Fujita...

  15. Viral Vectors 101: Virus Safety

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    ... on the Addgene blog Viral Vectors 101: Types of Viruses Viral Vector Elements Tips for Preventing...components of a virus into different plasmids. For a retroviral system this looks like the genetic cargo in one... researcher.   Figure 1: Separation of retroviral packaging components onto distinct plasmids for... contact with than GFP. Similarly, if the viral vector carries an shRNA to a gene essential for cellular...cellular function, this would also be a higher risk vector. While it is unlikely that you, or anyone else ...classification. Lentivirus (a type of retrovirus) and gamma retrovirus require more caution as they are integrating...common types of viruses grown in the lab are gamma retrovirus, lentivirus, adenovirus, and adeno-associated...

  16. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ...biosensor affinity for calcium.”  Primary Vector Type The vector type should describe how your plasmid ...indicate type in “Vector Type Other” column. For example, you may enter “donor template vector”. Cloning...expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain...plasmids containing the ccdB gene, such as Gateway vectors, choose the ccdB Survival strain. For plasmids ...

  17. Single-cell tracking of lineage and identity with CellTag

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    ...cell types. MEFs were reprogrammed into iEPs by retroviral expression of transcription factors Foxa1 and.... Adding Mettl7a1 to the standard Foxa1-Hnf4α retroviral overexpression cocktail yielded a three-fold ... lentivirus guide Sign up to receive our Viral Vectors 101 eBook ...

  18. Choosing Your Perfect Empty Backbone

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    ...fusion protein. Mammalian vectors One of our most popular mammalian expression vectors, c-Flag pcDNA3, comes...will need accessory packaging vectors to generate the virus. Bacterial vectors This system is great for overexpressing...cases bacterial expression vectors are inducible (e.g. pBAD LIC cloning vector (8A)) and have an epitope...folding assays. Yeast vectors There are several types of yeast expression vectors. Consider your applications...shuttle vectors. You may prefer the more traditional approach of an autonomously replicating vector that ...conduct your custom experiment. Fly vectors The vast majority of fly vectors are based on p-element, random... to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g. pLKO.1 - TRC cloning vector). Check out our...

  19. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

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    ... Our first popular "kit," Andrew Fire's set of vectors for C. elegans research was our most requested ...expression, such as pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -  If you're familiar with...requests grow for lentiviral packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two...control, and empty backbones such as pBABE-puro for retroviral expression and pLVTHM, a lentiviral plasmid for...

  20. Call for Guest Bloggers

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    ... purification Replica plating Anti-CRISPR Retroviral vectors Career development for scientists A new plasmid...

  21. Plasmids 101: Using Transposons in the Lab

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    ...integrates in human cells at rates similar to retroviral vectors. The hyperactive version of the SB transposase...integration efficiency in human cells similar to retroviral vectors. The hyperactive PB transposase (hyPB) is...in viral vectors, but easily fit in a TE. Transposons are also less likely than viral vectors to induce...transposon’s LTRs, similar to when packaging viral vectors.   Let’s take a closer look at three different ...transfer Transposons are an alternative to viral vector delivery of transgenes, like for iPSC reprogramming... piggyBac, which is a big advantage over viral vectors (~5 kB payload for AAV and ~8 kB payload for lentivirus...into new target sites. This is similar to how retroviruses, like HIV, replicate. Class I TEs do not encode...

  22. Viral Vectors 101: Viruses as Biological Tools

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    ...Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ...The most well-known retrovirus is HIV. Lentiviruses are a genus of the retroviral family that can infect... Viruses are essentially DNA (or RNA) delivery vectors, so what makes them better than the alternatives...measures in a separate, upcoming post. If a viral vector seems like the right approach for your experiment...experiment?           Click Here to Find Viral Vector Resources at Addgene! References and Resources... J, et al. “Altering the tropism of lentiviral vectors through pseudotyping.” Curr. Gene. Ther. 5 (2005...types of viruses used in mammalian research: 𝛄-retrovirus, lentivirus, adenovirus, and adeno-associated...

  23. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    ...body and thereby correct the mutations using retroviral vectors. However, many diseases require techniques...makes AAV vectors especially amenable to correcting disease-associated mutations. AAV vectors maintain... Check out our Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV...target cells has been challenging. A variety of vector delivery technologies have been investigated including...usability in clinical settings. On the other hand, vectors derived from AAV have recently gained popularity...profiles as well as other benefits. What makes AAV vectors great gene delivery tools? There are a variety ... like adenovirus for productive infection. AAV vectors are engineered to provide additional safety benefits...

  24. 5 Tips for Troubleshooting Viral Transductions

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    .... Viral vectors undergo DNA rearrangements. Depending on the composition of the viral vector and the ...about Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages ... one point or another. After amplifying a viral vector, collecting virus, and transducing target cells...the repeat sequences present in the LTR, the vector may undergo genomic rearrangements during replication...genomic rearrangements, try amplifying the viral vector with bacteria designed to minimize rearrangements...have low titers (which is unavoidable for some vectors) you can concentrate your virus by ultracentrifugation...antibiotic whose resistance gene is encoded in the viral vector. This antibiotic selection can be performed approximately...

  25. Viral Vectors 101: Pseudotyping

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    ...lentiviral vector will infect, pseudotyping can help. In pseudotyping, you produce viral vectors using viral...Sandoval S (2017) Pseudotyped Lentiviral Vectors: One Vector, Many Guises. Human Gene Therapy Methods ...lentivirus, retrovirus, and rabies virus. How to pseudotype To pseudotype your viral vector, you use the...et al, 2020). Because retrovirus is closely related to lentivirus, many retroviral envelope proteins have...To deliver genes using lentiviral vectors, you need an envelope protein on the virus’s surface and a ...Sometimes this is due to the cells that the viral vectors were produced in. For example, production of vesicular...another virus with rabies glycoprotein. Lentiviral vectors are also great at infecting neurons and cause minimal...

  26. Getting the Most from Your Lentiviral Transduction

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    ...conditions on the production of lentiviral and retroviral vectors.” Human Gene Therapy Clinical Development...About Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional...“Downstream processing of oncoretroviral and lentiviral gene therapy vectors.” Biotechnology Advances ... by the envelop protein. Many times lentiviral vectors are engineered with non-native envelopes, a process...titration of pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009): 495-505. PubMed PMID...Reiser, J. “Altering the tropism of lentiviral vectors through pseudotyping.” Current Gene Therapy 5.4...of the transductional efficiency of lentiviral vectors: effect of sera and polycations.” Molecular Biotechnology...

  27. Plasmids 101: Terminators and PolyA signals

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    ...Enhances Expression of Transgenes  Delivered by Retroviral Vectors. Journal of Virology. 1999. PubMed PMID: ...terminator will suffice. Many commercial expression vectors use double terminators to reduce unwanted translation... handled with care (4). For this reason, viral vectors often employ other non-poly(A) transcript-stabilizing...

  28. Retrovirus Plasmids

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    ...Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want a retroviral plasmid ...Viral Plasmids γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety...Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an RNA virus comprised...FAQ) about retrovirus Popular γ-Retrovirus Envelope and Packaging Plasmids Common γ-retroviral envelope ...mutagenesis). Read our γ-Retrovirus Guide for more information about: γ-Retroviral packaging systems Frequently...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...your gene of interest? Search our database for retroviral transfer plasmids that have inserts. ID Plasmid...

  29. Viral Vector Guides and Plasmids

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    ...capacity. Gamma-retroviral Vector Guide | Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred...Guides Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral...expression. Retroviral vectors are popular for making stable cell lines or clinical use. Adenoviral Vector Guide...Viral Vectors Resources Viral vectors are popular tools for delivering genetic information to cells. These...and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are versatile tools because...the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated viral (AAV) vectors are commonly used ...referred to as just retroviral vectors, have a relatively high transduction efficiency and only infect dividing...

  30. Plasmids 101: The Promoter Region – Let's Go!

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    ...lentiviral and retroviral constructs and we will discuss these in a future post on viral vector parts. Promoter... Can be induced by IPTG. Commonly found in pET vectors. Very tightly regulated by the lac operators. Good...-inducer fucose Weaker. Commonly found in pBAD vectors. Good for rapid regulation and low basal expression...Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and GFP...

  31. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    ....g. Mammalian) Vector Type: What type of experiment are you planning? (e.g. Retroviral) Species: Specify...but I can’t browse through 300+ plasmids. Under ‘Vector Types’ I selected ‘Lentiviral’ which eliminates...Identify a specific marker protein Use: Similar to “Vector Type” you can filter by experimental purpose (e.g...

  32. PiggyBac-ing Through the Genome Editing Field

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    ...quickly transfer a DNA sequence from the transposon vector to one of many TTAA sequences distributed throughout...encourage you to deposit your piggyBac™ transposon vectors with us to help us expand this useful resource....edit of interest. With the larger piggyBac™ donor vector, it is possible to make the ITR and selection cassette...splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites.,” Nat. Protoc., vol. 4, no. ...Transposase Read up on Genome Engineering with FLEx Vectors  Learn how Homology Directed Repair Is Used for...Genome Editing Resources on Addgene.org Find Vectors with the piggyBac™ Transposon Catch up on Everything...CRISPR with Our CRISPR Guide Deposit Your piggyBac™ Vectors! ...

  33. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones...pFastBac Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab ...expression vector with N- or C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF...Gateway destination vector for bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal...-1-3xMyc - Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc...His-tagged versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent...

  34. How to Deposit Your Plasmids with Addgene

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    ...the vector backbone you cloned your insert into and fill out the appropriate boxes with the vector backbone...backbone manufacturer, vector backbone size, total size of your vector + insert, any modifications you ...provide any full or partial plasmid sequences and any vector maps. The more sequence data available, the better... was a part of the insert (when cloned into the vector backbone). If your plasmid has more than one insert...you made to the backbone, and the vector type. Pro Tip! Reference the Addgene ID in the "Backbone Manufacturer...enter "Feng Zhang (Addgene plasmid #52961)".   The vector type should describe how your plasmid is intended...in E. coli, then this is a Bacterial Expression vector. If your plasmid can be classified in multiple ...

  35. Hot Plasmids: Spring 2025

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    Blog Post
    ...fusions for membrane display, they recommend the NGR vectors for most experiments. These new tools promise to... toolkit is the empty plasmid backbone: the DNA vector that contains everything needed to replicate inside... Looking for more? Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set for expressing...successful experiments or therapies. Using AAV vectors with enhancers that drive cell-type-specific expression...colleagues characterized a series of new enhancer-AAV vectors targeting specific classes of cortical and striatal...

  36. SARS-CoV-2 Pseudotyped Virus

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    ...Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly...Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing...HIV-based lentiviral particles Murine leukemia-based retroviral particles Vesicular stomatitis virus particles...-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen. NL4-...3 mCherry Luciferase - Lentiviral dual reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized...coronaviruses (184 KB) Addgene Blog Posts Viral Vectors 101: Pseudotyping Antibody Neutralization Response...Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus Envelope and Packaging Plasmids Reporter Plasmids...

  37. Five Popular Model Organisms

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    ...Zuber et al., 2009). By injecting mice with retroviral vectors that harbored different mutations found in...Gerald Rubin’s lab who created a set of modular vectors that can be used to optimally control gene expression...

  38. Plasmids 101: Expressing shRNAs for RNA Interference

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    ...processing Following delivery into cells using viral vectors or plasmids, shRNAs hijack the endogenous miRNA...expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs are transiently...cloning strategies for constructing shRNA expression vectors. BMC Biotechnology, 6(1). https://doi.org/10.1186...

  39. Plasmids for Stem Cell Research

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    ...lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral vector for "hit...MMLV-derived Retrovirus Human Yamanaka factors for generating human iPS cells; retroviral expression of...MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS cells; retroviral expression...Lentivirus Human Single polycistronic lentiviral vector for the expression of human Oct4, Klf4, Sox2, and...polycistronic, doxycycline-inducible lentiviral vector for the expression of human Oct4, Klf4, c-Myc, ...transcriptional activating fusions in single lentiviral vectors for human iPSC generation OCT4 and SOX2 Work as...Polycistronic, doxycycline-inducible lentiviral vectors for the expression of mouse Oct4, Klf4, c-Myc, ...

  40. Luciferase Plasmid Collection

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    ...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...Groskreutz 18760 MSCV IRES Luciferase Firefly Retroviral expression of firefly luciferase and gene of ...Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest upstream...and renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions upstream...212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating regions controlling transcription... Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating regions controlling transcription...pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic promoters. Daniel...

  41. Tetracycline Inducible Expression

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    ...plasmid. A single vector approach may not have as much flexibility and control as a dual vector design, but ...popular empty backbones, expression vectors for transactivators, and vectors for other applications, or search...Lentiviral Tet-Off vector for repressible expression with blasticidin selection. *Note: this vector contains an...rtTA-Advanced Rudolf Jaenisch 25434 pMA2640 Retroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin... Takuji Iwasato 63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre...Schiedlmeier, B. (2011). Retroviral and transposon-based tet-regulated all-in-one vectors with reduced background...respectively. You should also choose between: Dual vector system: express the transactivator and the tet-...

  42. Mammalian RNAi Tools

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    ...shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred method for long-term ...Plasmid Vector Type PI Publication Additional Resources Addgene Resources pLKO.1 cloning vector protocol...long-term gene silencing. Once the viral vector is taken up by a host, the viral genome is transcribed, producing.... shRNA Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a lentiviral... lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive expression...Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design in pSico with...

  43. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    Blog Post
    ...Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools Viral Vectors 101:...create far safer viral vectors that can be used in BSL-1 or BSL-2 labs. Viral vectors are viruses that have...increase the viral vector’s carrying capacity. In order to allow the viral vectors to reproduce when experimentally...expressed via cells cultured with the vectors. This allows the viral vectors to reproduce only in experimentally...of Viral Vectors 101 posts about each type, so be sure to check our blog, or our Viral Vectors 101 eBook...lentivirus, gamma retrovirus, and adenovirus. And now that you know what viral vectors are, it’s time to...viral particle is called a virion. Finally, viral vectors, which are used in the lab, are distinct from viruses...

  44. Viral Vectors 101: Types of viruses

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    ...Viral Vectors 101: Viral Vector Elements Viral Vectors 101: An Introduction to AAV Viral Vectors 101:...Maetzig T, Galla M, Baum C, Schambach A. Gammaretroviral vectors: biology, technology and application. Viruses...viruses: gamma-retrovirus, lentivirus, adenovirus, and adeno-associated virus. Gamma-retrovirus Retroviruses...type of gamma-retrovirus is Murine Leukemia virus (MLV). Naturally isolated gamma-retroviruses have been ...Lentivirus is a type of retrovirus and shares many common features with gamma-retrovirus including genome architecture... nucleus as gamma-retroviruses do. Fun fact: Since lentivirus and gamma-retrovirus are so closely related...expression (Robinson, 1982). To add insult to injury, retroviruses generally have moderate to high immunogenicity...

  45. Viral Vectors 101: Viral Applications

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    Blog Post
    ...Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ...We offer a variety of viral vectors, viral services and even a Viral Vectors eBook to get you started. ... terrains. They are, in fact, a choice delivery vector from basic science research all the way to clinical... long-term, and even inducibly depending on the vector and virus. Viral CRISPR-Cas9 tools have been...interest, turning expression off permanently. Viral vectors can also be used to express proteins. Viral expression...of interest, and often a selectable marker. This vector can express your construct in target cells transiently...mechanisms require a specific modification of the viral vector for your gene of interest – an shRNA sequence specific...

  46. Viral Vectors 101: Transductions

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    ...Production at Addgene Viral Vectors 101: What is a Virus? Viral Vectors 101: Viral Vector Elements ... First, a viral vector is selected to perform the transduction with: either retrovirus, lentivirus, adenovirus...desired DNA to be integrated can be cloned into the vector that you have selected. The nucleic acid will be...produced, and viral particles are packaged, when the vector is expressed. Once the design and construction ... it’s time to make virus! The constructed viral vector containing the cargo of interest is then typically...layers of risk management when working with viral vectors. Typically, one day after transfection into the...variety of broadly applicable and popular viral vectors. Check out our viral selections to see if we have...

  47. Viral Vectors 101: Biosensors

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    ...engineered into viral vectors, so if you want to use them, you’ll need to use the vectors (or redesign the ...Biosensors Viral Service: Biosensor AAV Viral Vectors 101 eBook Resources on the Addgene blog An Overview...For one, many viral platforms, such as AAV or retrovirus, can either permanently integrate and/or express... sensor, whether you choose lentivirus, gamma retrovirus, or AAV as your vehicle, we’ve got you covered...

  48. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...TP53 Exon Mutation Libraries 229137–229140 Donor Vector Human Stiewe N/A Varies 1,910–3,034 per exon Human...GBC) 85968 Barcode Human Weissman 3rd N/A N/A Retroviral Mouse Genome-wide CRISPR Knockout Library 104861...104861 Knockout Mouse Teichmann N/A (retroviral) 5 90,230 RNA-Binding Protein Pooled CRISPR Knockout Library...

  49. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...viral vector systems based on retroviruses like Moloney murine leukemia virus (MMLV). The vectors could...Lentiviral vectors are one of the most popular and useful viral vectors in the lab. Advantages of lentivirus...dividing and non-dividing cells. Lentiviral vectors are the vector of choice for many CRISPR applications,...and future) applications of lentiviral vectors! Lentiviral vector history and components In the early ...adeno-associated viral vectors can also target non-dividing cells, lentiviral vectors can carry much more...Lentiviral Plasmid FAQs. Lentiviral vectors in the lab Lentiviral vectors are incredibly popular in the lab...genome-wide screening.   Lentiviral vectors in the clinic Since lentiviral vectors can deliver a large amount ...

  50. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ...use viral vectors for many experimental purposes. To help you make sense of all the viral vector information...Subscribe to Viral Vector Blog Posts from Addgene Need more information on viral vectors? Check out our ...our viral vector guide pages, our Lentivirus FAQ, and our viral service pages. Click on the Image Above ... viral vector...some of the most important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this...
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