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  1. Distribution to Industry

    Type
    Collection
    ...growing collection of plasmids that are available to industry. Learn more about the available plasmids and ... Distribution to Industry Featured Collections Search Plasmids Browse Plasmids Browse...Addgene's Search to find plasmids available to industry scientists while also adding additional filters...of the plasmids that are currently available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit...us know what you would like to see available to industry scientists. Please note this does not guarantee... information on pricing, payment, and billing. Industry Material Transfer Agreements Most Addgene orders...documents, your order will be processed and shipped. Industry Distribution Policy A portion of Addgene’s collection...

  2. AAV Viral Preps

    Type
    Collection
    ...each shipment. If you can't find the prep you need, try Addgene's AAV Packaged on Request service. You can...

  3. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...editing blog post . Need help designing a pegRNA? Try the DeepPrime (Link opens in a new window) design...

  4. CRISPR References and Information

    Type
    Collection
    ...et al. (2016) (Link opens in a new window) . Also try CRISPOR batch (Link opens in a new window) for lentiviral...

  5. Immunocytochemistry

    Type
    Protocol
    ...type and the protein of interest. You may need to try a variety of fixation methods to find the best conditions... Protocols Immunocytochemistry Immunocytochemistry You may also like... Antibody Plasmid...Antibody Purification Protocol Introduction Immunocytochemistry is a technique that uses antibodies to detect...

  6. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry postdoc in neuroscience at...talked to people with industry experience, the more I heard that getting into industry early would only help...to postdocs in both industry and academia. Pros and cons: The academic vs. industry postdoc  Time: Academic..., many believe that industry postdocs, having already been immersed in an industry environment, have a...biotech. In other words, industry postdocs already have the prerequisite “industry experience” that I’ve ...as a requirement on some industry job postings. Also, if you work in industry for any length of time, ...be higher in an industry setting. Scientific community and mentorship: As an industry postdoc, you might...

  7. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...compartment, you may want to try an SDS-containing detergent like RIPA. You can try a few different lysing ...proteins to migrate…so don’t try eliminating one component entirely. If you are trying to transfer proteins of...10–15 V/cm of gel), your gel may be overheating. Try running it in a cold room or putting ice packs in... an issue, which can happen with large proteins, try lowering the voltage and using a longer run time....weird issue and can’t figure out what’s causing it, try making fresh running buffer. Old or improperly made... in different pore sizes for different proteins: try using a 0.2 µm (psi) membrane for small proteins ...voltages.  If your small protein is over-transferring, try using the faster semi-dry transfer, which works better...

  8. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...permeabilization techniques — and so it may be necessary to try multiple techniques. For example, we have found ...interest (Figure 1). It can therefore be helpful to try all three when looking to label an intracellular ...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Validation Resources...determine selectivity of an antibody for flow cytometry experiments. This will include a discussion of...detected in the intended application — here, flow cytometry — is the desired target, using one or more of ...antibody that has already been validated for flow cytometry through this approach. If you’re looking for a...Science) initiative has now started producing flow cytometry characterization data for selected antibodies,...

  9. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...your video call.  Expect technical difficulties. To try and circumvent this, test out the video conferencing...Congratulations, you’ve just landed an interview for an industry job! You’ve worked hard to earn this opportunity...you feel a little panicked. You’ve never had an industry interview before. What should you do to get ready...recently started working in the pharmaceuticals industry after earning my Master’s in 2016 and then working...between applying for an academic position versus an industry position is the on-site interviewing process. ...groups without having a formal interview process. Industry interviews, however, were more structured. I met...is what I’ve found helpful for my interviews in industry. Prepare stories to tell in your interview The...

  10. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...faster to perform. Introduction to flow cytometry Flow cytometry allows users to analyze single cells in... cultures.  Interpreting flow cytometry data To interpret flow cytometry data users will typically start...   Tips for flow cytometry success Break up cell clumps Success in a flow cytometry experiment depends...protocols? If this sounds like you, then flow cytometry can help you overcome these challenges and make...power in their observations. However, while flow cytometry provides analysis at the cellular level, it cannot...higher the level of expression. A routine flow cytometry experiment uses 2 or 3 different fluorescent colors...accommodate up to 30 different colors. This lends flow cytometry particularly well to complex experiments looking...

  12. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Conventional vs...When you are running flow cytometry, you’ll need various controls to help you set up and analyze your...Flow specific controls The three types of flow cytometry controls we’ll discuss are single color, fluorescence...linked on their commercial antibody pages. Flow cytometry can be a complicated and daunting process. Controls... vs. Spectral Flow Cytometry Resources on Addgene.org Addgene's Antibody Guide Browse Addgene's Antibody...

  13. Plan Your Experiment

    Type
    Collection
    ...identify the genomic sequence for the gene you are trying to target. However, the exact region of the gene...

  14. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...cells (visualized via the FITC channel) and you are trying to see what percentage of total live cells are ... biorender.com.   In such cases, instead of trying to quantify based on percentage positive or negative...Flow Cytometry Conventional vs Spectral Flow Cytometry Beyond Surface Labeling in Flow Cytometry Resource...example of a histogram plot for a yes/no flow cytometry readout. Created with biorender.com.   Using...

  15. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...that your gating strategy will be based upon. We’ll try to keep it short though, promise! FSC and SSC: scattering...When using flow cytometry to analyze your samples, it is necessary to set up a sequence of gates to be...the area under the curve (A).   While flow cytometry can be used for virtually all kinds of experiments...group already have experience with using flow cytometry, or if you have access to a professional flow ...learned the basic principles of gating in flow cytometry. Let’s briefly recapitulate what we’ve covered...resources on the Addgene blog Antibodies 101: Flow Cytometry  Antibodies 101: Reading a Flow Plot Antibodies...Antibodies 101: Conventional vs. Spectral Flow Cytometry...

  16. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...these dyes, as this would create difficulty when trying to resolve those populations. PE/Cy7 and BB515,...   Spectral flow cytometry Full spectrum flow cytometry, or “spectral flow” cytometry, on the other hand...panel! Although conventional flow cytometry and spectral flow cytometry are more alike than they are different...which spectral flow cytometry requires special considerations. Conventional flow cytometry During conventional...Flow cytometry is one of the most powerful tools available to immunologists, allowing for the rapid analysis...cell types. In recent years, full spectrum flow cytometry (“spectral flow”) has emerged as a particularly...conventional flow cytometry, light emitted by a fluorophore is guided to a single detector using a series of...

  17. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...your budget? Email [email protected] to try out the JF dyes. Acknowledgements I thank Brett ...Howard Hughes Medical Institute. Chemistry is dead, long live chemistry! The discovery of green fluorescent...biochemists have developed techniques to make labeling chemistry easier and more functional in complex biological... ligand to a peptide tag (Figure 1c). Click chemistry (e.g., transcyclooctene–tetrazine)– Nonnatural...bioorthogonal chemical reactions (i.e., click chemistry) to attach a fluorophore at the specific site ...groups—including mine—have been revisiting the old chemistry of dyes. The first synthetic dye, mauvine, was...net-neutral dye scaffold due to its established chemistry, small size, brightness, and cell permeability...

  19. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    ...What's a lab group in industry? The collaborative nature of working in industry and how delegating can...based in Switzerland. After completing her PhD in Chemistry and Chemical Engineering with Harry Gray at Caltech...Caltech, Crystal moved on to a Postdoc in the chemistry department at MIT. Crystal now works on protein...Crystal’s exciting career path, to gain insight into industry research, and to get advice on how to succeed ...Advice to scientists who are planning on working in industry. The importance of recognizing and leveraging ...adapting to needs as they arise. Tips on applying for industry jobs and deciphering job listings. Mobility between...

  20. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ...available to industry scientists  While we’ve focused on expanding COVID-19 plasmids to industry scientists...available to industry scientists To make plasmid sharing more open for academic and industry scientists,...available more broadly: to scientists in academia and industry. As a result over 90% of Addgene’s COVID-19 resources... to Addgene’s new approach of working with the industry-based scientific community. Conversations with...to the scientific community - both academic and industry scientists. This partnership complemented each...we have other plasmid collections available to industry? Over the past 8 years, several organizations ...Addgene and would like to make them available to industry, please reach out at [email protected] ...

  21. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...time before the experiment. What fixed it was not trying even harder to start earlier, but simply moving...work harder - it's to work differently.  Tools to Try  Checklists Found in surgical suites, air fields,...physical copy on hand as you run your experiments. Try drawing out a protocol as you read it, or putting...you to use cold block systems or buckets of ice. Try finding ways to intentionally set up your bench to...for you to reduce the chances of making an error. Try a variety of things, including (and maybe especially...

  22. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ... test them all, so how do you know which ones to try?  Read on and we will provide you with some advice...acid, for example in a Western blot. If you were to try and use such an antibody in an assay for a native... will bind to your primary antibody. If you are trying to visualize the precise location of a protein ...primary:secondaries:conjugates complex. If you are trying to visualize a protein in low abundance on a Western..., and other information to make sure you’re not trying to choose between the same antibody sold under ...be variations within an application. Take immunohistochemistry (IHC), for instance. While an antibody may...anti-tag antibody; and immunoprecipitation-mass spectrometry strategies that isolate an antibody’s immune...

  23. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...you should give similar workshops a try if you’re ever looking to try out a new technique or reagent! ... bit more about high throughput DNA prepping by trying out NEB’s new NEBNext Ultra II FS DNA Library Prep...technology. Then we split into pairs to actually try out the kit itself. This was great because it forced...

  24. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ... a lot of activities (try for a dozen!). The best approach may not be “let’s try to make this STEM activity...faster than it can be officially regulated. I did not try to sugarcoat the potential dangers of people using...not. But seeing some students really get it, and trying to answer some of their great questions, was immensely...difficult-but-rewarding jobs, and an enthusiasm for coming back and trying again next year. There are many reasons why you...resources: Steam Powered Family  Biology and Chemistry activities from Quatr.us Foldscopes Steve Spangler...

  25. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...career development. I try to do a really good job in one area at a time and try to say “no” to other activities...your home and family unless you live alone. If you try to do it all alone, it should be obvious that your...step! There is more than one way to do most tasks – try to resist the impulse to control by insisting things...tell them they will miss you, of course they will. Try really hard not to do this. For a family with working...

  26. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...cloning, Gibson Assembly, Golden Gate etc.), and try to find the simplest way to do it (i.e. avoid assembling...incubation, and amount of DNA. As a general rule, try to use an excess of insert compared to the backbone... fragments If you don’t get any positive clone, try transforming the ligation mix in different bacterial... you might want to reconsider your approach, and try a different cloning strategy. Once you've identified...

  27. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...your primer length and increase the Tm if you are trying to clone from genomic DNA, a cDNA library, or by...to conduct 2 ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid...colonies on your recipient plasmid alone plate, you can try ligating the recipient plasmid alone in the presence... that your transformation worked. You could also try varying the amount of recipient plasmid to insert...

  28. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ... seemed fuzzy or ill-defined. In this article, I try to clarify what people mean when they ask about “...back to the beginning, reassess the situation, and try a new strategy. Step Five: Share your results.  ... and ask them to explain their logic. Or you can try applying the scientific method to everyday problems...room to improve. The next time you face a problem, try to be mindful of how you work through it. Ask yourself...

  29. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...be familiar with the specifics of your research. Try to avoid jargon in this initial introduction. During...something you missed during the conference, you can try getting up to speed on social media. If you haven... when you are presented with a sea of strangers. Try things like sitting at a table for lunch with people...networking happens everywhere! Even when you aren’t “trying to network.” For some more networking tips not ...

  31. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...lessons I had learned – call it “troubleshooting.” Trying out new experiments and new ideas is nothing new... a bit about recruiting scientists to outreach.  Try new things to further your career Take advantage ...departments Graduate school was an excellent time to try new things, develop new skills, and meet new people... the Indiana State Museum and others when I was trying to find local informal science educators, and ever... mine was geared toward careers in academia or industry. Luckily, I discovered that there are many ways...

  32. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...to conduct 2 ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid...colonies on your recipient plasmid alone plate, you can try ligating the recipient plasmid alone in the presence...you are plating on the appropriate antibiotic and try varying the amount of recipient plasmid to insert...

  33. Bacterial Expression Systems

    Type
    Collection
    ...precision in control of gene expression so be sure to try a few different systems with your gene/protein of...

  34. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...KB If you have trouble loading this video, please try viewing on YouTube . Abstract The prokaryotic clustered...adherent cells, aspirate media. Add 20 μl of 0.05% trypsin-EDTA to each well with a clone present. Resuspend...Maeda T, Paw BH, Orkin SH. Journal of Biological Chemistry . 2014. PubMed . Correction of a genetic disease...

  35. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...During my first year of college, I knew I wanted to try research, but had no idea what to expect. I had a...university to perform research   Why should I try research? Personally, I have always been a pretty... when I was 18. I just knew I was interested in trying research, and if that’s where you’re at too, it...important.  As you’re talking to people, though, try to be conscious of who has your best interests at... and ever-expanding. While it can be tempting to try to plan out a very distinct and certain path, embracing...next career stage (ex: grad school, med school, industry) Other roles you might see Lab manager: ...not limited to working at a bench in academia or industry, so feel free to explore! Addgene has an entire...

  36. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...this what you mean? Are you trying to say this?" I'd say, "No, I'm trying to say X." She would then look... how many times I say this, candidates regularly try to convince me that their search committee is different...across that is not covered, please email me and I'll try to answer your question and subsequently update the...

  37. Clear and Accessible Writing

    Type
    Blog Post
    ...you’ll identify your purpose in using that image. Try using the questions: What is the goal, who is my ...be different for them when I’m done? What are we trying to fundamentally change about their knowledge?    ...together Now that you’ve gone through this process, try writing your own alt text for the example image. ...

  38. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...about their needs and any problems that they are trying to solve. After learning more about the stakeholders...software product management. Early in your career: Try to get software product management internships, preferably...you can’t find internships at science companies, try to find internships with translatable skills: working...

  39. The time and cost required to make a plasmid

    Type
    Blog Post
    ... a toss-up as to whether it’s faster to order or try to make it in your lab (but if you need a lot of ... no guarantee you’ll get a response on the first try (or any response at all). And while this method is...high-throughput” Instead of making one plasmid at a time, try constructing several plasmids in parallel. That way...

  40. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...buying and try to inversely pair brightness with abundance whenever possible.      Fig 2: Try to inversely...other signals. But PE can be very helpful if you're trying to find a relatively rare protein in an experiment...January 31, 2022, from https://www.bu.edu/flow-cytometry/files/2013/06/Fluorochromes-Brightness-Chart.pdf...

  41. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...what the result was, so try not to do this. These are the simple rules that I try to follow in my own research... Can Use to Save Time and Track Your Experiments Try out the CRISPR Software Matchmaker Additional Resources...

  42. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ... if you take time when you need it, rather than trying to push through a state of panic. Now, if I feel...you, it’s noteworthy and memorable and awful. So try to keep some perspective. Most people won't notice...did was public speaking. If you're also nervous, try taking a few deep breaths. Then, identify the specific...

  43. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...to conduct 2 ligations for each plasmid you are trying to create, with varying ratios of recipient plasmid...backbone + insert, unsuccessful 2 and 3 above), you can try ligating the recipient plasmid alone in the presence...you are plating on the appropriate antibiotic and try varying the “recipient plasmid : insert ratio” in... Addgene Blog: Learn How to Verify Your Plasmid Try Out Golden Gate or SLIC Cloning Read Other Plasmids...

  44. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...priority: location or efficiency. If you are just trying to shred a long non-coding RNA, cutting most anywhere...will be achieved. On the other hand, if you are trying to knock-in a patient mutation or need to make ...efficiency — although both are of course preferred! When trying to strike that balance, you may wonder: how close...

  45. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...science education Do you aim to reach young people? Try networking with your local education community. Get...ATJCagan or create agar art with colorful microbes? Try your hand at photography and participate in a science...Nothing prepares you better than learning on the job. Try to get an internship at a magazine or newspaper (...

  46. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...non-selectively. This non-selectivity is helpful when trying to pass tough-to-permeabilize membranes and these...IF and if a protocol is available. When possible, try to start with conditions and reagents that have already...the only way to know if a method will work is to try it. As you embark on your next IF adventure, just...integral membrane proteins. Journal of Histochemistry & Cytochemistry 33:813–820. https://doi.org/10.1177...

  47. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...have a gene of interest but have run into a wall trying to study it? It happens. Is it an evolutionarily...stop you from embarking on a fruitless journey of trying to get males to recombine two mutations onto the...not always clear which side he roots for. You can try to convince him to use Twitter @jonchowphd but he...

  49. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...solution to the problem you called about. Next, you try an email, given that this is the 21st century and... direct service you’ve come to expect from us.   Try out the Help Center! The Help Center can be found...

  50. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...in a specific role or cycle. For example, if you try to lead when you are expected to be doing, you may... (in particular your boss) that you are ready to try operating in a different role?  I think the secret...
Showing: 1 - 50 of 535 results