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Showing: 1 - 20 of 71 results

  1. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ..., developed and deposited at Addgene by Arlene Sharpe’s lab, allow for wide deployment of in vivo gene...Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested...Montero Llopis, P., Haining, W. N., Doench, J. G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible..., Al Abosy, R., Freeman, G.J., Haining, W.N., & Sharpe, A.H. (2019). PTPN2 regulates the generation of..., Coxe, D. J., Doench, J. G., Haining, W. N., & Sharpe, A. H. (2019). A CRISPR-Cas9 delivery system for...

  2. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ...engineering, Dr. Jennifer Doudna and Dr. Emmanuelle Charpentier. I was joined by Addgene Scientist Matt Ferenc...and Joan Steitz. This award ceremony honored Charpentier and Doudna, who talked about their research leading...simpler 1 protein - 1 RNA system Drs. Dounda and Charpentier met at a 2011 conference and decided to collaborate...was a simpler 1 protein - 1 RNA system.  Dr. Charpentier advocated for the crucial role of basic research...

  3. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    ..., developed and deposited at Addgene by Arlene Sharpe’s lab, enables in vivo screens in naïve T cells....Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested..., Hacohen, N., Doench, J. G., LaFleur, M. W., & Sharpe, A. H. (2024). Framework for in vivo T cell screens...

  4. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...experiments involve needles or scalpels (sharps). Cuts and scrapes from sharp equipment are one of the most common... yourself via needlestick or cut. Following all sharps procedures for your organization will significantly...

  5. Typing CRISPR Systems

    Type
    Blog Post
    ...science.adi1910 Chylinski, K., Makarova, K. S., Charpentier, E., & Koonin, E. V. (2014). Classification and...Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, D. H., Horvath, P., Moineau, S., Mojica... S. A., Alkhnbashi, O. S., Brouns, S. J. J., Charpentier, E., Cheng, D., Haft, D. H., Horvath, P., Moineau...

  7. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...denaturing.  Designed ankyrin repeat proteins, or DARPins, are genetically engineered small affinity proteins...binding proteins known as ankyrin repeat proteins. DARPins typically consist of 3-4 repeats, have high specificity...

  8. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...potentially shuttling repair down a different pathway (Charpentier et al., Nat Com).     Fig. 3: HDR events... 8(2024), (2017). 10.1038/s41467-017-01836-2 Charpentier, M., Khedher, A. H. Y., Menerot, S., Brion, A...

  9. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ... While You Work Dispose of sharps in a designated sharps container. Sharps include anything that can pierce...protection or face shields Eye wash Safety shower Sharps container Biohazard waste container Fire blanket...including, but not limited to broken glass or needles. Sharps containers must be thick walled, impenetrable by...

  10. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...frequency in a reference of highly expressed genes (Sharp and Li, 1987). In 2000 the Ikemura lab created the...Genet 12:683–691. https://doi.org/10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure...

  12. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ... authors were exploring were space and robots. Warping to stars in space ships and the rise of killer ...doi.org/10.1534/genetics.111.131433 Doudna JA, Charpentier E (2014) The new frontier of genome engineering...
Showing: 1 - 20 of 71 results