...nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a... vitro transcription none, need Cas9 plasmid Chen Do you have suggestions for other plasmids that should...

...nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a...vitro transcription none, need Cas9 plasmid Joung Do you have suggestions for other plasmids that should...

...repression experiments use targets within promoters. Do you have gRNAs you'd like to add to the Addgene collection...ID Plasmid Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that should...

...immunoprecipitations of your favorite gene even if you do not have an antibody against it. Another common...incorporated into the host DNA. For mammalian cells, you can do this through viral vector delivery. Visit our... Backbones AAV High transduction efficiency, but do NOT integrate into the host genome Addgene has an...Addgene Adenoviral High transduction efficiency, but do NOT integrate into host genome pAdTrack-CMV... Return to top Content last reviewed 28 May 2025. Do you have suggestions for other plasmids that should...

...A to G (or T to C) change. Adenine DNA deaminases do not exist in nature, but have been created by directed.../Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that should...

...nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a...Ingestion by C. elegans none, need Cas9 plasmid Ristow Do you have suggestions for other plasmids that should...

... RABV. These projection neurons do not express G-protein, and thus do not give rise to G-protein coated...

...lentiviruses or other retroviruses , adenoviruses do not integrate into the host genome. Disadvantages...full-length adenovirus genomes Bunz Return to Top Do you have suggestions for other plasmids that should...

...Systems, Harvard University.    Which titer do people usually report? Addgene and other viral vector...values to determine accuracy and reliability. When doing this, we make a judgement call on whether the two...improvement over qPCR (Taylor et al., 2017). What do Addgene AAV titers mean for your experiments? Because...reordering the same virus feel free to email us and we can do our best to send you a particular lot (if we still...Figure 2). Because physical titers are used for dosing purposes in preclinical studies, it is important...each lot of AAV in vivo to determine the optimal dosage. At Addgene, we don’t reserve lots, but if you’...

... by Alyssa Cecchetelli and Andrew Hempstead. What do smell, taste and sight have in common, besides being...

...nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a... Cas9 plasmid O'Connor-Giles, Harrison, Wildonger Do you have suggestions for other plasmids that should...

...sequence starts with a 'G', you naturally have one and do not need to add an additional 'G'. Where are double...HR) template, you could then: Confirm your HR by doing Restriction Fragment Length Polymorphism (RFLP) ...DMSO or without 5% DMSO. My genomic PCR didn’t work, do you have any suggestions? The Zhang lab recommends... the Science paper ( Cong et al. , Science 2013 ) do you have any suggestions? To date, I have never used...

...nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can be paired with a...HindIII S. cerevisiae LEU2 none, need Cas9 plasmid Lu Do you have suggestions for other plasmids that should...

...Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that should...

...Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that should...

...Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that should...

...Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that should...

...Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that should...

...Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that should...

...Description Gene/Insert Vector Type PI Publication Do you have suggestions for other plasmids that should...