Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 46 results for: Dre recombinase

Showing: 1 - 20 of 46 results

  1. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    ... using multiple recombinases. To make multiple, precise genome edits, the recombinases used must have ...excision of the stop sequence. In contrast, Dre recombinase, the close relative of Cre, displayed an excision...determine the cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested...however, the other recombinase/reporter pairs were inactive, even at very high recombinase levels. These data...toxicity of these recombinases by crossing flies homozygous for a UAS-driven recombinase with flies heterozygous...tubulin-GAL4. For Dre, Flp, KD, R, B2 and B3, approximately 50% of offspring carried both the recombinase and GAL4...recombination in Drosophila, read on to learn about new recombinases suitable for this system. Gerald Rubin’s lab...

  2. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Collections Recombinases Cre-Lox and Other Site-Specific Recombinases Cre Plasmids Flp Plasmids Dre Plasmids...your favorite site-specific recombinase (Cre, Flp, Dre, other recombinases). Use the search bars or sort...expressing Dre and other site-specific recombinases. Return to top Other Site-Specific Recombinase Plasmids...Plasmids Other Recombinase Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries...into two distinct families, serine recombinases and tyrosine recombinases, based on sequence similarity and...cerevisiae , respectively. Other tyrosine recombinases include Dre, R, Nigri, SCre, VCre, and Vika. These...highlighted plasmids expressing Cre , Flp , Dre , other recombinases , as well as useful empty backbones or...

  3. Recombinases AAV Preps

    Type
    Collection
    ...Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse the following...James M. Wilson Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA Syn none...collection of site specific recombinases. Viral... Viral Vector Packaging Service AAV Recombinases Viral Vector Packaging...in-stock AAV preps that encode site-specific recombinases, which can be used to control gene expression...Fan , Addgene Research Program Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI ...EF1a none 1, PHPeB Hongkui Zeng 140136 pAAV-EF1a-iDreV EF1a none 1, PHPeB Hongkui Zeng 140137 pAAV-EF1a-iFlpV...

  4. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...inventory of recombinases and recombinase-dependent vectors. We recently released several recombinases in various...pENN.AAV.hSyn.Cre.WPRE.hGH (105553-AAV5) Dre vector AAV phSyn1(S)-DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo...Calcium sensors and GABA sensors Optogenetics Recombinases And more! Control AAV For many AAV experiments...pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (124651-AAV9) Recombinase AAV Targeting and manipulating specific, genetically-defined...requested samples of AAV for testing purposes. To address this, we recently released our serotype testing...

  5. Retrograde AAV viral preps

    Type
    Collection
    ...Flpo recombinase Recombinases Li Zhang 121675 pAAV-EF1a-fDIO-Cre EF1a Flp-dependent Cre recombinase Recombinases...Alexander Nectow 50363 AAV phSyn1(S)-DreO-bGHpA Syn Dre expression Recombinases Hongkui Zeng 51669 AAV phSyn1...buttons. Controls Green Red Switch Other Recombinases Cre Flp VCre Dre Calcium Sensors GCaMP8f GCaMP8s GCaMP8m...expression Recombinases James M. Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn Cre expression Recombinases James...expression Recombinases Rylan Larsen 107788 AAV.rTH.PI.Cre.SV40 rTH Cre expression Recombinases James M....expression Recombinases Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Karl Deisseroth...expression Recombinases Karl Deisseroth 55638 pAAV-EF1a-vCre EF1a VCre expression Recombinases Karl Deisseroth...

  6. Deisseroth INTRSECT Collection

    Type
    Collection
    ...dual-transgenic recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing...-EF1a-mCherry-IRES-Dre None No 183535 pAAV-CaMKIIa-Flpo None Yes Single recombinase-dependent Addgene ...-iC++-EYFP Flp AND NOT Cre Yes Dual recombinase-dependent: DREADDs Addgene ID Plasmid Logic Sites and ...expression plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral...Intersectional expression approaches combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral...intersectional cell population definitions using recombinases. A) Cre and Flp are multiplexed to enable intersectional...Fenno et al., 2014. INTRSECT INTRSECT (intronic recombinase sites enabling combinatorial targeting) is a ...

  7. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...under the control of Flp recombinase together with inducible Cre recombinase (CreERT2), visualizes individual...Kranz, Andrea et al. 2010. “An Improved FLP Deleter Mouse in C57Bl/6 Based on FLPo Recombinase.” Genesis...incredibly popular and powerful site specific recombinase (SSR) system, but it only gives you a single ...control. What is FLP FRT and how does it work? FLP recombinase, which is named after its ability to invert or...a similar fashion to the Cre system. The FLP recombinase recognizes FRT sites and initiates site directed...However FLPe is still less efficient than Cre recombinase. FLPe codon optimization resulted in FLPO, which...Cre (Kranz et al. 2010). These differences in recombinase efficiency are important to keep in mind when...

  8. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ... EGFP Recombinase AAV Finally, we are excited to add Flp-expression AAV to our recombinase AAV inventory...Flp-expressing AAV. See our recombinase AAV inventory here New recombinases Plasmid Serotype Name ...inventory. Flp is another useful recombinase that can be used in combination with Cre for intersectional... µL size serotype testing) pAAV-hSyn-EGFP Recombinases Plasmid Serotype Name 55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo...FDA-approved drugs. See our inventory of DREADDs / Chemogenetics AAVs New DREADDs Plasmid Serotype Name 75033...Name 119036  AAV1  pAAV-hsyn-flex-Voltron-ST DREADDs / Chemogenetics AAV We have also expanded our inventory...activating and inhibiting neurons. Minimally-tagged DREADD AAV from the Richie lab are especially useful for...

  9. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...pAAV-hSyn-DIO-hM3D(Gq)-mCherry Bryan Roth   Recombinases Recombinase systems such as Cre-lox allow for more...types, recombinases allow you to further restrict gene expression to areas in which the recombinase and ...and construct are coexpressed. Three new recombinases from the Cepko Lab are now available as AAV1. CRE-...83896 AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell-5 83897 AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 ...SARS-CoV-2 proteins ChR2 for targeted expression DREADDs GFP dependent Cre and FLPo And more!  New lentiviral...plasmid encoding a Cre dependent version of the DREADD hM3D is now also offered as AAV1 (along with AAV...AAV2, AAV5, AAV8, and AAV9). Once activated, this DREADD acts through the Gq pathway and promotes neuronal...

  10. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ... in recombinase-dependent sequences. Recombinase-dependent sequences may be subject to recombinase-independent...date, we have analyzed hundreds of viral vectors and estimate that recombinase-independent recombination...pre-recombined population that does not require the recombinase for transgene expression. Once packaged in AAV...pre-recombined DNA could lead to transgene expression in recombinase-negative animals, producing misleading results...transgene expression but limits the likelihood of recombinase-independent expression. Next-generation sequencing...

  11. AAV Viral Preps

    Type
    Collection
    ...Chemogenetics DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre,...Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins...

  12. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...site-specific serine recombinase. First, the prime editor installs the recombinase “landing sites,” then...then the recombinase handles the installation of the donor sequence. The latest work evolves and engineers...engineers Bxb1 recombinase variants to optimize PASSIGE efficiency (Pandey et al., 2024). The best-performing...editing installs two sequences containing attB/P recombinase landing sites on opposite DNA strands. When the...edited sequence with the attB/P landing site. A recombinase integrates a sequence from a donor plasmid containing... in mammalian cells via continuously evolved recombinases and prime editing. Nature Biomedical Engineering...low-abundance targets or natively isolated complexes. To address these limitations, the Funabiki Lab developed a...

  13. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ...only become activated in the presence of Cre recombinase, which is limited to the hippocampus. Thus, only...titers for efficient retrograde transport. For recombinase-dependent vectors, this can cause challenges ...impact of this phenomenon is to use a retrograde recombinase-expressing vector (e.g. Cre or FLP) to transduce...projection pathway neurons and then inject a recombinase-dependent chemogenetic vector directly into the...the neck bone,” but with about a hundred billion neurons and six hundred trillion connections (synapses....

  14. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...viral vector is used to deliver DNA encoding Cre recombinase under the control of the synapsin promoter. It... cre-recombinase...running, and you can learn more about this useful, DREADD-containing AAV here. But the top requested AAV ...

  15. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ... PMID: 30678704. Sparse cell labeling using recombinase competition Article contributed by Aliyah Weinstein...Weinstein Sparse Predictive Activity through Recombinase Competition (SPARC) is a fully genetic tool for...binds to calcineurin-dependent response elements (CDREs) and drives gene expression. Because calcineurin...-degraded destabilized GFP variant fused to a 4X CDRE repeat. In addition to showing that this vector ...

  16. Brain Initiative Collection

    Type
    Collection
    ...expresslight-inducible site-specific iDreV recombinase Hongkui Zeng 140136-PHPeB pAAV-EF1a-iDreV can be used to generate...light-inducible site-specific iCreV recombinase Hongkui Zeng 140136-AAV1 pAAV-EF1a-iDreV can be used to generate ...will expresslight-inducible site-specific iDreV recombinase Hongkui Zeng 140137-AAV1 pAAV-EF1a-iFlpV can...83894-AAV1 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...83894-AAV2 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...83894-AAV5 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...83894-AAV9 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...

  17. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Cre Recombinase James M. Wilson AV-1-PV2676 105553-AAV1 pENN.AAV.hSyn.Cre.WPRE.hGH Cre Recombinase James... Cre Recombinase James M. Wilson AV-2-PV1090 105537-AAV2 pENN.AAV.CMVs.Pl.Cre.rBG Cre Recombinase James...Cre.SV40 Cre Recombinase James M. Wilson AV-5-PV2408 105550-AAV5 pAAV.GFAP.Cre.WPRE.hGH Cre Recombinase James... Cre Recombinase James M. Wilson AV-8-PV1090 105537-AAV8 pENN.AAV.CMVs.Pl.Cre.rBG Cre Recombinase James... Cre Recombinase James M. Wilson AV-9-PV1090 105537-AAV9 pENN.AAV.CMVs.Pl.Cre.rBG Cre Recombinase James...pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 Cre Recombinase James M. Wilson AV-9-PV2394 107788-AAV9 AAV.rTH.PI.Cre.SV40 Cre Recombinase James M...Cre Recombinase James M. Wilson AV-9-PV2676 105553-AAV9 pENN.AAV.hSyn.Cre.WPRE.hGH Cre Recombinase James...

  18. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...First, instead of using piggyBac™, you could use recombinase technology (Cre/loxP) to remove the donor cassette...[7], it is generally not as efficient as Cre recombinase. However, the use of negative selection largely...to DNA toxicity. More importantly, unlike Cre recombinase, the PBx is highly specific, and has not been... you will need to screen somewhere between one hundred and one thousand clones to find a cell line with...

  19. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...2003). This is achieved through site-specific recombinases such as Cre or Flp that induce DNA recombination... "Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents." Scientific reports...recombination. Purification and properties of the Cre recombinase protein." Journal of Biological Chemistry 259.3...26611576. PubMed Central PMCID: PMC4971574. Hooper, Andrew, and Jamie Maguire. "Characterization of a novel...

  20. Caltech Systemic Capsids

    Type
    Collection
    ...light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible recombinase iDreV Zeng 140137..._ChR2_mCherry BiSSTe4 Activator ChR2 Fishell Recombinases 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn...140137 pAAV-EF1a-iFlpV EF1a light-inducible recombinase iFlpV Zeng 164450 CN1851-rAAV-hI56i-minBglobin-...Cre-dependent DREADD Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor, Cre-dependent DREADD Roth 135635...-Gq-P2A-dTomato E2 regulatory element Activator DREADD Dimidschstein Biosensors 104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE...
Showing: 1 - 20 of 46 results