...RNA samples are amplified by PCR instead of RPA. Unlike RPA, this technique uses Taq polymerase which is...isothermal recombinase polymerase amplification (RPA) using a commercially available amplification kit...regions of gene S and Orf1ab gene are amplified. RPA reactions also contain reverse transcriptase to produce...fluorescence upon cleavage. Upon amplification by the RPA primers, the Cas12a-crRNA binds to the complementary...nucleic acids, the target sequence is amplified by PCR/RPA using biotinylated primers. These are then immobilized...

...amplified using RPA (recombinase polymerase amplification) or reverse transcriptase (RT)-RPA, respectively...respectively. RPA is coupled with T7 transcription to convert amplified DNA to RNA for subsequent detection by ...first amplified through isothermal amplification by RPA. When Cas12a-cRNA base pairs with the dsDNA of interest...

...Recombinase Polymerase Amplification (RPA) or Reverse-transcription RPA (RT-RPA), respectively. Amplified nucleotides...

...largely underutilized compared to their Class 2 counterparts like Cas9. This is attributed in part to the...

...sequences, the amino acid sequences RSIAT and RPACKIPNDLKQKVMNH have been used successfully (Kerppola, 2006...between BiFC constructs and their endogenous counterparts using western blotting. The subcellular localization...be compared against those of their endogenous counterparts (by immunocytochemistry, for example), as this...

...approximately 25% of yeast genes have a human counterpart, enabling study of human-relevant pathways (Liu...grows at higher cell densities than most of its counterparts, yielding higher production power from a given...

...CRISPRko (knock out), CRISPRi (interference), and CRISRPa (activation) libraries that do just that (Doench... Find CRISPRko (Brie), CRISPRi (Dolomiti), and CRISRPa (Caprano) libraries for the mouse genome! Other...

...myofibroblasts to cell death via down-regulation of TRPA1 channels. British Journal of Pharmacology, 178(15... antibodies for the specific detection of human TRPA1. Scientific Reports, 9(1), 18500. https://doi.org...

...creativity, cleverness, and crafting skills have surpassed your colleagues. Success is sweet – as sweet as...

...did not localize similarly to their untagged counterparts. These findings motivated Georgieva et al. to...

...again reached new heights on the Addgene blog: we surpassed 60,000 views for the month of September! Historically...

..., plasmids, and depositing (all while our US counterparts are still sleeping). Plasmid sharing in Europe...

...llamas, and alpacas (and their undomesticated counterparts) - not exactly species that jump to mind when...

...systems that may have a leg up on their modern counterparts. What are traps? Traps – gene, enhancer, and...

...mammalian plasmids differ from their bacterial counterparts, including how replication occurs and whether...

...This property makes WYL proteins an intriguing counterpart to anti-CRISPR proteins that negatively modulate...

...and its gRNAs are also larger than their Cpf1 counterparts. Table 2. Cas9 Multiplexing Options  Multiplexing...

...changes creating 2 other versions but these did not surpass GEmCherry2 in background fluorescence and in-frame...

... the appropriate light. The photoactivatable counterpart to this system utilizes a gRNA which is photocaged...

...CDK5 all behave similarly to their endogenous counterparts. Additionally, dimeric Dronpa is less prone ...