We narrowed to 954 results for: TES

... CRISPR Parasites CRISPR Plasmids: Parasites Cut Resources Eukaryotic parasites continue...use in parasites. CRISPR...cause substantial death and disease in the world. Parasites such as Plasmodium (malaria), Toxoplasma (toxoplasmosis...propelled both reverse and forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also...also been established for some of these parasites. Together, these tools have enabled precise genetic ...

...Service AAV Serotype Testing AAV Viral Vector Packaging Service: Serotype Testing AAV These AAV encode...EGFP vectors in various serotypes for serotype testing: AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, and...Inventory . The catalog ID for the 20 µL sized testing sample is denoted with a .T suffix (for example... (for example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use...

...repository. Browse the University of Florida serotype testing panel for the eye and brain....Packaging Service AAV University of Florida Serotype Testing Panel for the Eye and Brain Viral Vector Packaging...Packaging Service: University of Florida Serotype Testing Panel for the Eye and Brain You may also like: Viral...by ddPCR, in vitro and (when possible) in vivo testing, and full sequencing of the final viral vector ...injection in mice and macaque. This serotype also demonstrates enhanced transduction of cells in culture for... with improved mGluR6 promoters in rodents and primates. Mol Ther . 2016 Aug;23(8-9):680-9. PMID: 27115727...

...proteins or fragments. NanoBRET target engagement (TE) assays (Link opens in a new window) have a similar...low-abundance proteins at endogenous levels in lysates or live-cells, thus preserving physiological relevance...and its target protein. The apparent affinity of test compounds is measured by competitive displacement...

...GJ, Ramakrishnan C, Kozicz T, Deisseroth K, Thiele TE, McElligott ZA, Holmes A, Heisler LK, Kash TL. 2016...recombinase recognition sequences (e.g. Lox sites, FRT sites) inside of the introns (A,D). The addition..., 2014. INTRSECT INTRSECT (intronic recombinase sites enabling combinatorial targeting) is a synthetic...portion of the ORF that is sandwiched between the sites; the starting direction of these ORF fragments (... in a new window) for the molecular design and testing of novel INTSRECT plasmids for groups interested...targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55636 pAAV-EF1a-Cre... recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55641 pAAV-Ef1a-fDIO...

...new target sites. Created in BioRender.com.   Class II TEs: DNA transposons Class II TEs are also ...of transposition: Class I TEs, also called retrotransposons, and Class II TEs, also called DNA transposons.... Class I Transposable elements (TEs): Retrotransposons  Class I TEs are also known as retrotransposons...the TE while also increasing the size of their host’s genome.   There are two types of Class 1 TEs: those...DNA sequences or target sites. Integration of most transposable elements (TEs) results in the duplication...into new target sites. This is similar to how retroviruses, like HIV, replicate. Class I TEs do not encode...encode a transposase enzyme.  Class I TEs are considered replicative since every time they jump they make...

...Figure 1. Visual representation of common injection routes in mice. Image created with biorender.com. ...skin and the muscles. The most common injection sites are flat and easily accessible, like on the back...provided a good introduction to common injection routes in mice! When considering what method you’d like...experiments! Table 1: Summary of common injection routes in mice. Injection Route Restraint Location... (2020). A retrospective analysis for different routes of administration in Mice-Percutaneous Retro-Orbital...doi.org/10.4236/jbm.2020.89011 Shimizu, S. S. (2004). Routes of administration. In H. Hedrich (Ed.), The Laboratory...Administration of substances to laboratory animals: routes of administration and factors to consider. PubMed...

...annual Deck The Lab holiday decorating contest! Our #DeckTheLab contest is a time for scientists worldwide...the details:   Contest 1: Deck the Lab - Classic Edition This edition of the contest is our traditional...December 23, 2020 Contest 2: Deck the Lab - Home Edition This edition of the contest should showcase a...for either contest on Twitter or Instagram by December 31, 2021. Let us know which contest you are entering...celebrate! And if you want to see how Addgene celebrates and decorates, follow us on Twitter and Instagram as... show off their decorating skills. To join the contest, simply embellish and adorn your lab space or your.... Have fun! Here’s an example from last year’s contest:   🎶Walking in a Winter Wonderlab🎶 We decided...

...everyone who participated in our annual #Deckthelab contest. The bar was set very high from last year’s impressive...chocolate for the lab. Here are some of our 2017 contest winners:   Chicagoland's Community Biology ...Stanford University was so moved by our deck the lab contest that she spent a year hoarding used tip boxes for...lab supply tree and set a challenge for her lab mates to create the best-recycled lab supply ornament ...blast decorating and creating memories with their labmates! Are you inspired by our 2017 #Deckthelab winners...winners? Be sure to enter our 2018 #Deckthelab contest when the next holiday season rolls around! ...

.... We asked all of the Addgenies to pick their favorites out of the bunch and here’s what ended up on top...stopped working on our reports/lab stuff for a few minutes to create this lovely winter scene out of stuff... of our entrants had fun decorating with their labmates! ...

...considerations, and give just the lightest of nods towards conjugates.  Storage   Once you get your exciting...antimicrobial.  Conjugates And HRP brings us handily to the final topic of this post, the conjugates, or signaling...questions is “yes,” then I would suggest either testing the antibody efficacy through a standard curve,...buffers, can bind to amine groups present in some conjugates, blocking the conjugation reaction. Many commercial...much every other assay you can do. Most non-HRP conjugates are fluorophores, which work not by reacting ...

...non-parametric test may be the way to go, which we will discuss more in another blog. T-tests T-tests compare...classic parametric tests and one of the simplest statistical tests available. Tailed t-tests If you are solely...ANOVA tests are essentially t-tests with room for more variables and groups. ANOVAs can test three or more...within a test, depends on the input data ‘following the rules’ of that test. All statistical tests have assumptions...distribution profiles.   Parametric tests  Statistical tests fall into two general categories: parametric... blog we will be discussing parametric tests. Parametric tests are suitable for data sets that “play by...need to be met for a parametric test are as follows:   Parametric test assumptions: Distribution – Normal...

...of the Federal Labor Standards Act (FLSA) which mandates that all workers across the country either be ...specifically chosen to better define productive routes for advocacy efforts and help participants develop...Fisher Scientific Developing effective science advocates is an important way to push the balance from challenge... advocates...

...learn more! Background: Argonaute and NgAgo In eukaryotes, Argonaute proteins play an important role in... RNA. Argonaute proteins are also present in prokaryotes, and some of these proteins protect against invading...structure can affect editing efficiency. Gao et al. tested if NgAgo preferred certain guide sequences - using...

...example, Cre with lox sites, Flp with FRT sites, Dre with rox sites), enabling precise genetic manipulation...region of DNA between the target sites is reversed. B) If the target sites are on the same DNA strand (in...between the sites is excised as a circular piece of DNA (and is not maintained). C) If the sites are on separate...Recognition Sites Each enzyme functions analogously but interacts only with their own target sites (for example... cell reconstitutes a functional protein, allowing recombination of the DNA between target sites. Codon...facilitate higher rates of recombination. Gene Expression Strategies Depending on how the target sites are arranged...recombinase recognizes and mediates recombination between the two FRT sites, resulting in the stable integration...

...protocols that recommend eluting in water, Tris-EDTA (TE), just Tris buffer, or some other variations. Does...experiments you can use your plasmid.  DNA elution choices: TE, Tris buffer, or water Figure 2: Tris-EDTA,...choose could affect your downstream experiment. TE (10 mM Tris-HCl,1 mM EDTA, pH 8.0) buffer is the ...In the situation where you need to use plasmid in TE for any enzymatic reaction, an effective possibility...adenine or guanine) from the DNA. These apurinic sites if in double stranded DNA are repaired by base excision...time. Tris buffer controls the pH, while the EDTA chelates any divalent cations like Mg2+, preventing the...

...acetyllysine reader BRD3R promotes human nuclear reprogramming and regulates mitosis. Nat Commun. 2016...and c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming to pluripotency...30. Zhou Astrocytes Dopaminergic Neurons Lentiviral Human Efficient conversion of astrocytes to functional...Fibroblasts Astrocytes Lentiviral Mouse Direct conversion of fibroblasts into functional astrocytes by defined...Oct4, Sox2, Klf4, and c-Myc from polycistronic cassettes. piggyBac transposition reprograms fibroblasts...To Delivery Method Species Article PI iPSCs Cardiomyocytes Lentiviral Human Robust cardiomyocyte differentiation...Sci U S A. 2012 May 29. Palecek Fibroblasts Cardiomyocytes Lentiviral Mouse Optimization of direct fibroblast...

...2013). We also have an older system using two cassettes to express the CRISPR RNA array and tracrRNA separately...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...loops at tetraloop and stemloop - Contains BbsI sites for insertion of guide sequences Addgene plasmids... and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of guide sequences Zhang Lab SAM Cloning...guide RNA: These plasmids contain two expression cassettes, a human codon-optimized SaCas9 driven by either...AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return to top...

...Fusions; Coding Prefix alleviates RBS-CDS issues. Prohibited Restriction Sites: EcoRI, XbaI, SpeI, PstI...genome. Prohibited Restriction Sites: EcoRI, SpeI, NheI, PstI Restriction Sites to Avoid: PvuII, XhoI, AvrII...assembly standards – combinations of restriction sites which preserve a standard prefix and suffix sequence... are added. Therefore, the same reaction which ligates a promoter into an empty backbone can be repeated...restriction enzymes used for assembly are the prohibited sites and must be omitted from the coding sequence of ...backbone. For some standards, there are additional sites which should be avoided whenever possible. Other...PstI Restriction Sites to Avoid: NotI For more info, visit iGEM: BioBrick (RFC 10) Back to Top BioBrick...