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We narrowed to 22 results for: Tet-On Advanced

Showing: 1 - 20 of 22 results

  1. Tetracycline Inducible Expression

    Type
    Collection
    ... of tet-responsive systems: Tet-Off and Tet-On. Read on to learn more about the components of tet systems...shown. Tetracycline Off (Tet-Off) The first major advance was the Tet-Off system. A tetracycline-controlled... center: Tet-Off; right: Tet-On. TRE: Tet response element; tTA: tetracycline-controlled transactivator...expression. These include: Tet-On Advanced : improved transactivator (rtTA-Advanced, also known as rtTA2 S...the CAG promoter for Tet-Off tTA-Advanced Takeshi Imai 104109 pAAV-Syn1-tTA AAV Tet-Off vector, expressed... Plasmid Collections Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression ...conditions, the TetR protein binds to tet O, blocking transcription of the downstream gene. If tet or one of...

  2. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...2nd generation Tet-Off vectors (Tet-Advanced)” are the most recent variants of the Tet-Off system.  For...gene-function/tet-inducible-expression-systems/tet-systems-legacy-products/tet-on-and-tet-off-2nd-generation...10.1101/gad.2.6.718. PMID: 2843425.Takara Bio. Tet-On and Tet-Off systems: Second generation. https://www.takarabio.com...promoters Tet-Off In its native context, the tetracycline repressor (TetR) can bind to the tetracycline operator...operator sequences (TetO), preventing transcription. In the presence of tetracycline (Tet), TetR preferentially...preferentially binds Tet over the TetO elements, allowing transcription to proceed (pJKR-H-tetR from the Church ...to promoters containing TetO elements (often linked in groups of seven as a Tet Response Element (TRE))...

  3. Mammalian RNAi Tools

    Type
    Collection
    ...that allow for conditional (Cre-lox) or inducible (Tet) expression are available. To find plasmids containing...siRNA vs. shRNA: Similarities and differences . Advanced Drug Delivery Reviews, 61 (9), 746–759. PubMed...

  4. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can serve as controls in...activate expression of a gene under the control of the tet operator. AAV AAVs are the workhorses of both chemo...communication and sharing will only help research advance. Remember: productivity is infectious!  Additional...

  5. Neurodegeneration Research Collection

    Type
    Collection
    ...works. The neurodegenerative disease field will advance more quickly with the avid sharing of data, tools... bioRxiv. 2022 Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription factors for...to helping researchers accelerate discovery and advance development of diagnostics and treatments for Alzheimer...

  6. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...technical developments. Using the Tet-on system, his lab has created a tetracycline cas9 inducible knock-out system...Initiative and how this initiative will lead to advances in human health. It was inspiring to hear how ...

  7. Cre-lox system

    Type
    Collection
    ... pBS537 tet-hCMV-GFPcre tet inducible Cre-GFP fusion tet-hCMV Mammalian Sauer 11961 pBS596 tet-hCMV-GFPcre...none Mammalian Sauer 11957 pBS595 tet-hCMV-EGFPcre Cre-EGFP fusion Tet inducible Mammalian Sauer 11958 ...Supernova TRE AAV Iwasato 85577 pTC-CMV-Tet CreER expression and tetracyclin-dependent transgene/shRNA expression...ApoE.HCR.hAAT Mammalian Ehmer 85578 pTC-ApoE-Tet CreER expression and tetracyclin-dependent transgene/shRNA expression...tet-hCMV-GFPcre tet inducible Cre-GFP fusion, metallothionein MT-I region including the polyadenylation site and...and several introns tet-hCMV Mammalian Sauer 12168 pMB80 (R26-CreER) Cre-ERT2 with loxp cassette; Targeting...Mammalian Messing 45359 pNK-TGCK Cre-EGFP fusion; Tet inducible - rrTA expression driven by mouse Nkx cardiac...

  8. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...synthesizing fluorophore fusions on their own. Subsequent advances in fluorescent proteins have replicated many of...sensitivity, photoactivation, etc. These impressive advances lead to an obvious question: In this age of GFP...dye structure have yielded commercial panels of advanced rhodamine fluorophores, such as the Alexa Fluor...properties inside living cells, especially for advanced imaging experiments. We continue to develop derivatives...rather be in the lab making new fluorophores for advanced imaging modalities. Follow him on twitter @rhodamine110...FlAsH, ReAsH) and a short genetically encoded tetracysteine (Cys4) peptide tag could be used to label proteins... lipoic acid ligase, biotin ligase, phosphopantetheinyltransferase) – These enzymes catalyze the covalent...

  9. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...organisms. Advances in tissue clearing Similar to light microscopy, there have been significant advances in tissue... beginning of this century has seen some major advances in light microscopy, particularly related to the...in 3D with unprecedented detail.  One of these advances in microscopy has been light sheet fluorescence...spinal cords by combining Ultramicroscopy with tetrahydrofuran-based tissue clearing (13,14). A few years ...

  10. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...Molecular Cytology and Van Leeuwenhoek Centre for Advanced microscopy, University of Amsterdam. The previous...Molecular Cytology and Van Leeuwenhoek Centre for Advanced microscopy (University of Amsterdam). He develops... In corals, the red fluorescent proteins form tetramers. In general, fluorescent proteins have a natural...

  11. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...has used an inducible MitoTimer reporter using a Tet-on system (pTRE-Tight-MitoTimer) to show its usefulness...proteomic mapping in living cells. Despite these advances, the Ting lab’s first-generation APEX suffered...repressor plasmids from Scot Wolfe's lab. These include Tet-inducible CRISPR activators and repressor plasmids...

  12. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...still, this challenge did not hinder them from advancing their careers and achieving success.” Pedro Gonzalez...would like to be in a few years, helping me to advance in my career.” Adrian Mancebo (Mentee IMP 2014-...University, the Salk Institute, and the Karolinska Institutet, among others). Mentors and mentees held scheduled...the International Mentoring Foundation for the Advancemnet of Higher Education website Resources at Addgene...

  13. Brain Initiative Collection

    Type
    Collection
    ...Initiative Collection The Brain Research through Advancing Innovative Neurotechnologies® (BRAIN) Initiative...Gradinaru 117383-AAV1 TRE-DIO-eYFP An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent...

  16. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...immunity with exosome-enveloped AAV vectors." Blood advances 1.23 (2017): 2019-2031. PubMed PMID: 29296848....Exosome-Associated AAV by Overexpression of the Tetraspanin CD9." Molecular Therapy-Methods & Clinical Development...

  17. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...dual-plasmid approach makes it a valuable resource for advancing our understanding of its biology, pathogenicity...on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker KanR....

  19. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...12605205. 13. Yan, Qi, and Marcel P. Bruchez. "Advances in chemical labeling of proteins in living cells... nonfluorescent until they bind a 6-residue tetracysteine tag (sequence: CCPGCC) that can be inserted ...interest, resulting in a C-terminal fusion of a tetracysteine tag. In addition, since these tags are so small...et al. "Hairpin structure of a biarsenical− tetracysteine motif determined by NMR spectroscopy." Journal...

  20. CRISPR Guide

    Type
    Guide
    ... higher efficiency and product purity. Science Advances . 3 (8), eaao4774. PMID: 28875174 Leal, A., Herreno-Pachón...- activation through increased H3K27 acetylation TET1 - activation through cytosine demethylation DNMT3A... a companion CRISPRon to undo repression using a TET1-dCas9 fusion. Figure 10: Overview of epigenetic ...
Showing: 1 - 20 of 22 results