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We narrowed to 48 results for: Tight TRE

Showing: 1 - 20 of 48 results

  1. Tetracycline Inducible Expression

    Type
    Collection
    ... of interest. Tight TRE promoter or P Tight : reduced background expression and tighter binding of Tet-Advanced...gene of interest. tTA-Advanced Tight TRE David Sabatini 19407 pTREtight2 Empty backbone for Tet-controlled... is unable to bind the TRE. In the presence of tetracycline, rtTA binds the TRE, promoting gene expression...for additional selection markers. rtTA-Advanced Tight TRE Eric Kowarz 96930 XLone-GFP Tet-On PiggyBac vector...cloning and puromycin selection rtTA-Advanced Tight TRE David Root 100521 pCW57.1-MAT2A Lentiviral Tet-Off...Tet-controlled expression (On or Off) None Tight TRE Markus Ralser 64238 pTet-IRES-EGFP Lentiviral plasmid for ...and EGFP. Pbi contains a TRE between two minimal CMV promoters. None Pbi (TRE, miniCMV) Bert Vogelstein...

  2. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... 87429 TJP1-mEGFP AICSDP-23 mEGFP Tight junction protein ZO-1 Tight junctions 91565 AAVS1-mEGFP AICSDP...AICSDP-64 mEGFP RNA-binding protein FUS Paraspeckles/stress granules 114411 TNNI1-mEGFP AICSDP-34 mEGFP Troponin...

  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...mApple-VE-Cadherin-N-10 Tight Junctions VE-Cadherin mApple Michael Davidson 55165 mCherry-ZO1-C-14 Tight Junctions ...reducing cell stress. There are four types of cell junctions: adherens junctions, gap junctions, tight junctions...Beta-Catenin mCherry Michael Davidson 30313 pEGFP ZO1 Tight Junctions Zonula Occludens-1 GFP Alan Fanning *Fusions...FAP; mCerulean3 Marcel Bruchez 45944 pTDpelB-C_sfYFPTwinStrep Periplasmic space PelB signal sequence YFP...

  4. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...both rhamnose- and arabinose-inducible promoters. Tight control of toxin expression is absolutely necessary...induction system is well-characterized and quite tight (Giacalone et al., 2006). The Chen lab demonstrated...routinely clone the purified amplicons upstream and downstream of the desired deletion directly into cut...transcriptional terminator BBa_B1002 (from IGEM) upstream of the MCS to reduce transcriptional readthrough...accessibility, selective RNA unfolding and sliding at upstream standby sites." Nucleic acids research42.4 (2013...expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system." Biotechniques...

  5. Cre-lox system

    Type
    Collection
    ...Stainier 85040 pK170.AAV-TRE-Cre-WPRE (Supernova) TRE-Cre based Supernova TRE AAV Iwasato 85577 pTC-...Bacterial Liu 63704 pRetroX GFP T2A Cre GFP and Cre tight TRE Retroviral Foijer 64769 pTW040 Cre fused to the...GFP and Cre CMV Lentiviral Lu 118029 pAAV-TRE-dDIO-vCre VCre TRE AAV Luo 119063 pBac(3xP3-gTc‚Äôv; Tc-hsp68...CAG Mammalian Zhang 68477 pTRE:iRFP670-EFS:Cre-2A-GFP iRFP670, Cre, and GFP TRE Mammalian Jacks 68544 AAV-Cre-GFP...to activate Cre. One advantage of this system is tight temporal regulation. Promoter-regulated Cre: The...Cre-containing Plasmids Scientists have developed ways to tightly control Cre expression and to optimize Cre expression...often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest will prevent gene expression...

  6. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    ...surprisingly more rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually...academic calendar Academic researchers must be extreme self-starters and especially good at self-promoting—No...especially with peer mentors, to discuss your strengths, weaknesses, interests and passions is another...

  7. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...promoter, and rabbit beta-globin splice acceptor. TRE General expression mRNA Tetracycline response element... region from trpB and -10 region from lac. Very tight regulation. Good for modulating gene expression ...are about 100 to 1000 base pairs long and found upstream of their target genes. The sequence of the promoter...of other promoter elements, and tend to be more tightly regulated. Common promoters for eukaryotes and ...induced by IPTG. Commonly found in pET vectors. Very tightly regulated by the lac operators. Good for modulating...increases expression of the repressor 10x, thus tightening regulation of lac promoter. Good for modulating...

  8. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ....  Viral vector injection Viral vectors allow a tight spatial and temporal control of gene expression ...into the experimental setup, the mouse becomes stressed. It will likely move around faster in the following...some time to adapt to a testing apparatus. General stress levels of the test animal may also be reduced by...noise should be avoided as this will also increase stress levels and affect behavior. A fact to consider:... groups), health status, diet, cage enrichment, stress level, origin (vendor or in-house mouse breeding...

  9. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...other BSL-2 materials, you’ll need to use aerosol-tight caps, compatible with your centrifugation speed,...does happen, ensure you can receive appropriate treatment and/or monitoring. Virus-specific concerns   ...

  10. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...supplementation with grant funds, money is always tight. Of course, unlike your traditional biology lab,...and Makerspaces back in 2007, which function as centres for peer learning and knowledge sharing, in the...

  11. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ...approach should you use if the budget is particularly tight? Most cloning processes will require a PCR reaction... game, however, or simply looking for a more streamlined approach, you might want to give Gateway a try...cloning kits can often make the process much more streamlined, because they will come with comprehensive and...your favor. For starters, Golden Gate cloning is extremely efficient due to the use of the versatile type...

  14. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...linked in groups of seven as a Tet Response Element (TRE)), allowing transcription to proceed. When tetracycline...Tet systems function well in mammalian cells, and TRE-containing promoters can be used in the repressible...References and Resources References Gossen M, Bujard H. Tight control of gene expression in mammalian cells by...GAL4 binding to UAS. If you place the GAL4 gene downstream of a tissue- or developmental stage-specific ...uncharacterized promoters. Similarly, placing UAS upstream of a transgene permits directed expression of ...

  15. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ... represents a straightforward means of exerting tight control over Cas9 nuclease activity while eliminating...conventionally used SpCas9 (derived from the bacterium Streptococcus pyogenes). Consequently, fewer genetic tools...complex. When this complex is directed to a target upstream of the transcriptional start site (TSS) of a gene...competent to cut DNA. When directed to a site downstream of the transcriptional start site of a gene of...

  16. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...meters long. You twist and roll the string into a tight ball. Now the two ends of the string, instead of...and stop. For instance (chr3, 1, 10) indicates a stretch of DNA at the very beginning of the third chromosome... Let’s now examine how we can locate powerful stretches of DNA known as enhancers with the help of genome...light on dark matter Precise knowledge of which stretches of the genome are pillars for stem cells, or metastasized...

  17. Lab to Office Culture Shock

    Type
    Blog Post
    ...times during a 10 minute conversation. Leaving a tight-knit team like that can and will be a little gut-wrenching...question today, because I sometimes wish for a stretch of time that doesn’t involve critical thinking....

  18. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...you to easily use a set of plasmids together on a tight budget. To help popularize our kit resources,  ...leader sequences (encoding small leader peptides) downstream of which you can clone your gene of interest....transcription from resulting in the production of a downstream peptide, the transcription terminators in this...BIOFAB Plasmid Set contain terminators of varying strengths centered within GFP/RFP bicistronic reporters ...

  19. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...inducible MitoTimer reporter using a Tet-on system (pTRE-Tight-MitoTimer) to show its usefulness in cell culture...fluorescence images of C2C12 cells stably expressing pTRE-Tight-MitoTimer and rtTA. The cells were pulsed with...Biotech. 2013. Dec 23.   Highly bright and extremely dim GFPs The availability of the three dimensional...unprecedentedly-high brightness, bfloGFPa1, and the other with extremely-dim brightness, bfloGFPc1. The plasmids used for...and Keith Shearwin labs have developed a new, streamlined approach to genetic engineering which drastically...plasmid after transformation. Addgene depositors Sylvestre Marillonnet and Nicola Patron have assembled two... N- and C-terminal tags (including His, S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, NusA and SUMO) for ...

  20. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ... sterile aluminum foil or a cap that is not air tight. Incubate bacterial culture at 37°C for 12-18 hr...Liquid Bacterial Culture You may also like... Streaking and Isolating Bacteria on an LB Agar Plate Creating...on your LB agar plates are not fresh, you should streak your bacteria onto a new LB agar plate before growing...
Showing: 1 - 20 of 48 results