Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening
Type
Blog Post
...MIT's Tim Wang and colleagues, including David Sabatini and Eric Lander, comprise 73,171 guide RNAs (gRNAs...genes and it includes more guides per gene. The Sabatini/Lander library uses sequential infection first...systematic genetic screens in mammalian cells,” David Sabatini, a senior author of one of the Science papers,... Using the CRISPR/Cas9 System. Wang T, Wei JJ, Sabatini DM, Lander ES. Science. 2013 Dec 12. (PubMed)....