We narrowed to 50 results for: astl

1. Plan Your Experiment

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   Collection

   ...generating modified mouse lines. Protein delivery Lastly, you can deliver Cas-gRNA ribonucleoprotein (RNP...pool of cells to find one with your desired edit. Lastly, you will need to know if your edit just affects...

2. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

   Type

   Blog Post

   ...signal to noise ratio five fold over the 1xHA tag.  Lastly, the researchers found that the HA frankenbody ...the ability to bind nascent HA tagged proteins.  Lastly, the HA frankenbody can be used for imaging in ...

3. Five Popular Model Organisms, Part 2

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   Blog Post

   ...modification tools and methods such as CRISPR have vastly improved scientists’ ability to use rats as disease...first, advances in axolotl genome sequencing have vastly expanded axolotl research. One of the most significant...

4. Antibodies 101: Designing Your First Flow Panel

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   Blog Post

   ...separately detect these markers in your analysis. Lastly, there are fixable dye stains that react with free...will now exclude FITC from the rest of our panel. Lastly, we want to detect the expression of our protein...

5. Using AAV for Neuronal Tracing

   Type

   Blog Post

   ..., and bidirectional transport for AAV1, -8, -9 (Castle et al., 2014). Differences between AAV serotypes...PMID: 25555356. PubMed Central PMCID: PMC4324159. Castle, M.J., Gershenson, Z.T., Giles, A.R., Holzbaur,...

6. We’re Going Viral This Week!

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   Blog Post

   ...increase AAV2 yields and we’re eager to share more! Lastly, we’ll be at SfN2019. If you’re attending SfN2019...

7. Bringing Sustainable Practices to the Lab: Easy Action Items

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   Blog Post

   ... do the same. Long-term lab conservation goals Lastly, I think it’s a good balance to have both short-term...

8. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

   Type

   Protocol

   ...not be possible depending on the type of sleeve. Lastly, don’t touch your face! Close-toed shoes and long...

9. Science Communication for Everyone

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   Blog Post

   ...and doctors with their questions and comments.  Lastly, we are planning to launch an e-magazine this year...

10. Understanding GPCR Signaling Using TRUPATH

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    Blog Post

    ...effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists and inverse-agonists...

11. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    Blog Post

    ...interest to create conditional human cell lines. Lastly, the Kanemaki lab demonstrated that auxinole, a...

12. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type

    Blog Post

    ...staining, a popular method to observe apoptotic cells. Lastly, the Shu Lab demonstrated that the FlipGFP-based...

13. 5 Great Apps for Lab Life

    Type

    Blog Post

    ... find the sequence for your enzyme of interest. Lastly, the Tm calculator helps you set up an optimal ...

14. 7 Tips to Secure a STEAM Internship This Summer

    Type

    Blog Post

    ... letter that is customized for each internship. Lastly, have your resume and cover letter revised by your...

15. A Quick Guide to a Career in Software Product Management

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    Blog Post

    ...asset to the scientists as well as the developers. Lastly, you’re going to need to be a problem-solver. A...

16. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type

    Blog Post

    ...through the presence of a fluorescent reporter. Lastly, once a mutant is identified, the PB transposase...

17. Hot Plasmids - February 2022

    Type

    Blog Post

    ...ability to knock out multiple genes in parallel. Lastly, this Cas12a-based screening method was used to...

18. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

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    Blog Post

    ...Information Systems Audit and Control Association. Lastly, Carl also serves on the Board of Advisors and ...

19. Multiplex Genome Editing with CRISPR-Cpf1

    Type

    Blog Post

    ...of a crRNA array that can be processed by Cpf1. Lastly, an AAV vector was used to multiplex edit 3-4 genes...

20. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    ...opposite strands. This “double nickase” strategy vastly reduces breaks and mutations at off-target sites...