...newly developed calcium sensors. Selecting a calcium sensor Genetically encoded calcium indicators (GECIs...unusually fast calcium dynamics, whereas non-neuronal systems tend to have slower, but larger, calcium changes... to use a calcium sensor that is most sensitive to your expected range. For example, calcium in the ER...would want a low affinity calcium biosensor, so that the (1) binding of calcium, (2) allosteric rearrangements...FRET-based calcium reporter derived from troponin C. It is engineered with a reduced number of calcium binding...’ve got a live one (calcium sensors for in vivo studies) There are several calcium reporters specially...Calcium is arguably one of the most important metal ions in biology (sodium and potassium are up there...

...transfer (FRET)-based calcium biosensors. Perhaps most notably, the Twitch calcium sensors, which are available...-affinity for calcium binding and developed a version of the protein with as few calcium-binding sites...been working on genetically encoded indicators of calcium and other small molecules since the very beginnings...engineered sensors were designed to replace synthetic calcium dyes, which had been in use since the 1980s. “Synthetic...they shift from "off" to "on" in the presence of calcium. This color change can be observed under a microscope...Griesbeck and his colleagues began by isolating a calcium-binding protein, troponin C (TnC), from muscle ...Ratiometric imaging for beginners Griesbeck says his calcium indicators can be used in mouse, Drosophila, C....

... of a given ion - we’ll use calcium biosensors as an example. Many calcium biosensors include GFP fused...region RS20. When calcium is absent, the construct does not fluoresce; when present, calcium induces CaM/RS20...like arabinose or hydrogen peroxide or ions like calcium and zinc. Some biosensors contain a single fluorescent... biosensors from Robert Campbell’s lab display calcium-dependent increases in fluorescence of up to 11,000...common class of biosensors. Blue flame “cameleon” calcium sensors created by Roger Tsien’s lab contain two...proteins (a FRET pair) separated by CaM and M13. When calcium binds, it triggers CaM/M13 interaction that increases...Examples include blue flame mitochondria-targeted calcium sensor CMV-mito-GEM-GECO1  and blue flame extracellular...

...ions, calcium is by far one of the most popular, with dozens of sensors developed for it. Calcium triggers...the presence of calcium. This includes FRET-based sensors such as Twitch. While calcium is king in the ...The calcium is strong here….I can sense it….sound like a plot to an biology scifi movie? Or maybe it’...such as norepinephrine, or an electrolyte such as calcium. This varied component, which we will refer to ...triggers muscle contraction, and calcium channels are activated for neuronal action potentials; it’s an ion... disciplines! Most of the sensors designed for calcium generate a fluorescent output, which will turn ... on the biology you are studying. For example, calcium dynamics in the cytosol and sarcoplasmic reticulum...

...pAAV-hSyn-mCherry Read on to learn more!  Calcium sensor AAV The GFP-based calcium sensors are single-wavelength ...sensors with intensities that change in response to calcium binding. There are a few versions of these jGCaMP7...brightest resting fluorescence of the GCaMP7 family of calcium sensors and can be used for imaging of small neuronal...variant of jGCaMP7 in AAV1 at Addgene! See all calcium sensor AAV at Addgene. GABA sensor AAV GABA is ... directly imaging GABA have been limited. Like calcium sensors, different iGABASnFR have been developed...pAAV-EF1a-mCherry-IRES-Flpo 55637  AAV1  pAAV-EF1a-Flpo Calcium sensors Plasmid Serotype Name 104496  AAV1...

... Biosensor AAV (calcium sensors and GABA sensors) Calcium sensors The latest calcium sensors from Janelia...include: EGFP-expressing AAV for serotype testing Calcium sensors and GABA sensors Optogenetics Recombinases...for monitoring neuronal activity in the form of calcium dynamics. (For more details on this technology,...technology, see our blog post Overview of AAV Encoded Calcium Sensors). We are expanding the inventory of these...pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5) Calcium Sensors pGP-AAV-syn-jGCaMP7b-WPRE (104489-AAVrg...

...pAAV-hSyn-DIO-EGFP   Biosensor AAV Calcium sensors Tracking calcium dynamics that govern such activities...studying neural networks. We have expanded our calcium sensor collection to include our first Flp-dependent...Several new options are also available for the calcium sensor jGCaMP7 from Janelia, including three jGCaMP7...technology, see our blog post Overview of AAV Encoded Calcium Sensors). Plasmid Serotype Name 104489 AAVrg...

...jRGECO1a Calcium Sensor Kim , GENIE 100854 pAAV.Syn.NES-jRGECO1a.WPRE.SV40 Syn jRGECO1a Calcium Sensor ...pGP-AAV-syn-jGCaMP7s-WPRE Syn GCaMP7s Calcium sensor Kim 104489 pGP-AAV-syn-jGCaMP7b-WPRE Syn GCaMP7b Calcium sensor Kim 104491...Cre-dependent Calcium sensor Kim 104493 pGP-AAV-syn-FLEX-jGCaMP7b-WPRE Syn GCaMP7b, Cre-dependent Calcium sensor...Cre-dependent Calcium sensor Kim 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG GCaMP7f, Cre-dependent Calcium sensor...GCaMP7f Calcium sensor Kim 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn GCaMP7f, Cre-dependent Calcium sensor...GP-AAV-syn-jGCaMP8s-WPRE Syn GCaMP8s Calcium sensor GENIE 162375 pGP-AAV-syn-jGCaMP8m-WPRE Syn GCaMP8m Calcium sensor GENIE 162376...GCaMP8f Calcium sensor GENIE 162377 pGP-AAV-syn-FLEX-jGCaMP8s-WPRE Syn GCaMP8s, Cre-dependent Calcium sensor...

...Gordon Fishell Calcium GCaMP5 genetically encoded calcium sensor Optimization of a GCaMP calcium indicator ...GCaMP calcium indicators. Nat Methods. 2009 Dec;6(12):875-81. Loren Looger Calcium NEMO calcium sensors...Polleux Calcium Red calcium sensor PinkyCaMP based on mScarlet PinkyCaMP a mScarlet-based calcium sensor...Schreiter Calcium GCEPIA1-SNAP ER for sensing ER calcium A genetically encoded ratiometric calcium sensor...Target Description Article PI Calcium jGCaMP8 Fast Genetically Encoded Calcium Indicators (Constitutive or...Encoded Calcium Indicators. Janelia Research Campus. Online resource. 2020 GENIE Project Calcium jGCaMP7...High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance calcium sensors for imaging...

...doi: 10.1101/2022.08.13.503850.  iGECInano NIR calcium reporter Near-infrared (NIR) fluorescent proteins...’s lab has generated a new genetically encoded calcium indicator using small monomeric NIR fluorescent...photochemical characteristics than previous NIR calcium reporters iGECI and NIR-GECO2G, and its small size...Characterization of a Small Near-Infrared Fluorescent Calcium Indicator. Front Cell Dev Biol. 2022 Jun 29;10:...response size and reduced sensitivity to pH and calcium compared to previous lactate biosensors, and its...additional AAV serotypes of the most recent jGCaMP8 calcium sensors: pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3...

..., including calcium sensors, under the control of various promoters. Sensor Green Calcium Sensors GCaMP8...soma-GCaMP6 Twitch2B Yellow Calcium Sensors jYCaMP1 axon-jYCaMP1 Red Calcium Sensors CaMPARI jRCaMP1 jRGECO1...viral service encoding biosensor tools, including calcium sensors. Viral...jRGECO1 sRGECO Far-Red/NIR Calcium Sensors HaloCaMP1a HaloCaMP1b NIR-GECO Acetylcholine Sensors iAChSnFR..._ACh3.0 Syn GRAB_ACh3.0 none Constitutive 9 Li Calcium Sensor: GCaMP8f 162376 pGP-AAV-syn-jGCaMP8f-WPRE...CAG jGCaMP8f none Constitutive 1, 9, rg* Looger Calcium Sensor: GCaMP8s 162374 pGP-AAV-syn-jGCaMP8s-WPRE...CAG jGCaMP8s none Constitutive 1, 9, rg* Looger Calcium Sensor: GCaMP8m 162375 pGP-AAV-syn-jGCaMP8m-WPRE...

...luciferase Many intracellular sensors of events like calcium release have been made using fluorescent resonance...generally consist of a responsive protein (e.g. calcium binding protein) fused to two fluorescent proteins...proteins. When the responsive protein binds to calcium it changes conformation and brings the two fluorescent... due to its bright luminescence. Intracellular Calcium Sensor Yang, J. et al. (7) wished to trigger intracellular...this optogenetic tool, they needed to create a calcium sensor with the following characteristics: No ...CalFlux VNT responds to changes in intracellular calcium and is useful for direct imaging of events in cells...

...SPARC to image the calcium response in the dendrites of T5 neurons by placing the calcium indicator GCaMP6f...intermediate (d), and sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe...

...and iGECI calcium indicator (Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators...performance, and applications of near-infrared calcium indicators in this recent review (Shcherbakova ... genetically encoded near-infrared fluorescent calcium ion indicator. Nat Methods 16, 171-174 (2019). ....A. et al. A near-infrared genetically encoded calcium indicator for in vivo imaging. Nat Biotechnol 39...

...Control AAVs for serotype testing jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic inhibition...high contrast between its resting state and its calcium-bound state, which was achieved by lowering the...  AAV1  pAAV.hSynapsin.SF-iGluSnFR.S72A New calcium sensors Plasmid Serotype Name 104496  AAV1...

...fluorescence protein-based calcium sensors (jYCaMP1), variants of the calcium indicator jGCaMP7, that are...with red fluorescence protein-based calcium sensors. These new calcium sensors include syn-jYCaMP1s (slow...

...robust, with an eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki et al... to previous publications. They also developed calcium, cAMP, and ATP sensors based on Nano-lantern, showing...

... CAG Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn Calcium sensor...pGP-AAV-syn-jGCaMP7f-WPRE Syn Calcium sensor GCaMP Kim , GENIE 135632 pAAV-S5E2-GCaMP6f E2 regulatory element Calcium sensor ...pAAV.Syn.NES-jRGECO1a.WPRE.SV40 Syn Calcium sensor RGECO Kim , GENIE 162375 pGP-AAV-syn-jGCaMP8m-WPRE Syn Calcium sensor GCaMP...Biosensors 104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104492 ...104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE...

...intermediate (d), and sparse (e) labeling of the calcium indicator, GCaMP6f, in the Drosophila optic lobe...phosphatase that responds to rising intracellular calcium levels within the cell. When levels of Ca2+ are...

.... Factor Xa loves calcium, but metal-chelate matrices, not so much. Maybe a calcium-independent proteinase...