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We narrowed to 81 results for: maz

Showing: 1 - 20 of 81 results
  1. Genetic Code Expansion

    Type
    Collection
    ...35TAG) PylRS M. mazei Cyanobacterial TAG Lital Alfonta 92048 gCOTS-pyl PylRS M. mazei Cyanobacterial TAG... PylRS N346A-C348A M. mazei Bacterial Wenshe Liu 137908 PylRS-AS PylRS M. mazei fluorophenylalanine derivatives...PylRS M. mazei AzHeK Bacterial TAG Wenshe Liu 140009 pAS_4xMma PylT_FLAG-Mma PylRS PylRS M. mazei CpK, AbK... PylRS M. mazei Bacterial TAG Lital Alfonta 160089 pPaGE Pyl TAG FliC T248TAG PylRS M. mazei Bacterial...PylRS M. mazei Mammalian TAG Simon Elsaesser 174896 pAS_4xMmaPylT(UUA)_EF1_MmaPylRS PylRS M. mazei Mammalian...PylRS M. mazei Mammalian TGA Simon Elsaesser 174901 pAS_MmaPylT_EF1_NES-MmaPylRS WT PylRS M. mazei Mammalian...synthetase M. mazei Mammalian TAG Ivana Nikić-Spiegel 182537 pEVOL-MmAcKRS1-PylTUUA PylRS M. mazei Bacterial...
  2. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...protein-based toxin-antitoxin system is MazE/MazF. The toxin MazF is a ribonuclease that cleaves specific...toxin-antitoxin systems and their applications MazE/MazF Aside from CcdB/CcdA, there are many protein-based...protein synthesis. The antitoxin MazE, which binds and inhibits MazF, is continually cleaved by the ClpAP...continual production of MazE, the toxin becomes active. Shen Yang’s lab made use of mazF as a counterselectable...pKSCTMF contains the mazF gene and a gene for Zeocin resistance (Figure 2). This MazF-ZeoR cassette is flanked... Stephen K. Burley. "Crystal structure of the MazE/MazF complex: molecular bases of antidote-toxin recognition...multiple genetic modifications in the same strain. MazF expression is under control of the AOX1 promoter...
  3. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression mAzurite 383 450 14 5 Monomer (A206K) mAzurite-N1 - Mammalian Expression mAzurite-C1 - Mammalian...Expression mAzamiGreen 492 505 41 5.8 Monomer mAzami Green-N1 (mAG) - Mammalian Expression mAzami Green-C1...
  4. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...lentivirus-based SARS-CoV-2 related pseudovirus assay. David Nemazee 170575 pCMV-FLuc Express firefly luciferase. Used... MLV-based SARS-CoV-2 pseudovirus assay. David Nemazee 161030 Zhou-pLOV-GFP-Luc Lentiviral vector expressing...Lentiviral Particles with SARS-CoV-2 Spike Protein Nemazee lab - Pseudotyped virus protocol for coronaviruses...
  5. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    ...work interviewing fellow Addgenies. I spoke with amazing people that were a part of the different departments...internship but in the end it turned out to be an amazing experience. I am thankful to have been given the...some of the most genuine people who have shared amazing advice with me. Due to my experience at Addgene...
  6. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...was with a sense of relief that I attended the amazing, postdoc-driven Future of Research Conference at...This fantastic city-wide effort was led by two amazing postdocs, Kristin Krukenberg and Jessica Polka ...change.  Dr. Bourne strongly believes this is an amazing time of promise for science—we have powerful tools...
  7. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ... Throughout these talks, I oscillated between amazement at how much has been done with CRISPR technology...type of genetic engineering is a testament to how amazing the downstream payoff could be. Though I attended...of this meeting who have given the attendees an amazing opportunity to share their cutting edge science...
  8. Addgene: The First Twenty Years

    Type
    Blog Post
    ...anniversary was about all the amazing years behind us, as much as it as about the amazing years in front of us....makes us… us. “The Addgene not-for-profit is an amazing service to biology scientists and has saved countless...
  9. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...coelenterzine substrate to the more stable yet brighter furimazine by Promega’s Chemistry group is a good story ...imaged after i.p. or i.v. administration of the furimazine substrate for NanoLuc luciferase. Both GpNLuc...into mouse livers followed by i.v. injection of furimazine or luciferin 24 hours later demonstrated a more...
  10. Zebrafish Plasmid Collection

    Type
    Collection
    ...expression system for use in zebrafish - Bret Pearson Lab MAZe: a tool for mosaic analysis of gene function in ...abundance, size, and localization in high-throughput. MAZERATI platform - Leonard Zon Lab. A platform to rapidly...
  11. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ... at our core mission. Plasmid distribution is amazingly still growing at >9% every year (12% in 2018) ...alumni Addgenies on our success. Thanks to our amazing team, we also don’t have to worry about being able...
  12. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...G-protein Optical (ONE-GO) biosensors (Janicot and Maziarz et al., 2024). Each plasmid expresses all the components...ONE-GO Biosensors plasmids here! Janicot, R., Maziarz, M., Park, J. C., Zhao, J., Luebbers, A., Green...
  13. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...neurons that project to a given brain region (Mazarakis et al., 2001).  This method can also allow scientists...Virol 28:e1963 . https://doi.org/10.1002/rmv.1963 Mazarakis ND (2001) Rabies virus glycoprotein pseudotyping...
  14. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...One of these behavioral tasks was an elevated plus maze that measures risk aversion/anxiety. The mouse can...were less risk averse. Similar to the elevated plus maze, the light-dark box is a simple device comprised...
  15. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...., Wu, W. L., Sánchez-Guardado, L., Lois, C., Mazmanian, S. K., Deverman, B. E., & Gradinaru, V. (2017... Campos, L. J., Griffiths, J. A., Ichiki, T., Mazmanian, S. K., Osborne, P. B., Keast, J. R., Miller, ...
  16. Mammalian RNAi Tools

    Type
    Collection
    ... Moradzadeh, A., Mehmandoost, N., Moazzen, F., Mazraeh, A., Marmari, V., Ebrahimi, M., Rashno, M. M., ...
Showing: 1 - 20 of 81 results