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We narrowed to 51 results for: rbis

Showing: 1 - 20 of 51 results

  1. Common Injection Routes in Mice

    Type
    Blog Post
    ...vein or retro-orbitally Gauge: 27–30 Volume: < 0.2 ml Skill required: beginner (retro-orbital) to intermediate...anesthesia Tail vein or retro-orbital 27–30 < 0.2 mL Beginner (retro-orbital) to intermediate (tail vein...injections.” However, they can also be given retro-orbitally, behind the eye. IV injections are used for a ... the correct vein without collapsing it. Retro-orbital injections are also thought to be less stressful...routes of administration in Mice-Percutaneous Retro-Orbital, jugular catheter, tail vein and femoral cut down...

  2. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...stopped talking to each other if I was not there to arbitrate for them. They were different people with some...leave everyone feeling vaguely unsatisfied. In arbitration, there is more of a winner and loser–one party...considered in the right, and one in the wrong. If you arbitrate, one party might get exactly what they want, but...punished or invalidated. Both compromise and arbitration might remedy short-term substantive conflicts...

  4. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...guest blogger Jon Backstrom, a biochemist in the Vanderbilt Eye Institute and Tonia Rex's lab. A common strategy...Backstrom. Jon Backstrom, Ph.D is a biochemist in the Vanderbilt Eye Institute and is particularly interested ...

  6. RUBY-Red Siliques

    Type
    Blog Post
    ...transformation fall into two main categories: antibiotic/herbicide resistance and fluorescent proteins. Selection...field conditions or in sterile conditions where disturbing the tissue for imaging or collection could contaminate...

  7. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...requires constant turbidity of the culture, so bacteria must be grown in a turbidostat. Only mutate particular...

  8. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...efficiency when delivering these viruses via retro-orbital vs. tail vein systemic injections? A: We have not...difference in AAV transduction efficiency between retro-orbital versus tail vein injections.   Q10: Have you or...

  10. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...resistance to the herbicide chlorsulfuron (Homo line 2) or wild type (WT), two weeks after herbicide was applied...

  12. AAV Production in HEK293 Cells

    Type
    Protocol
    ...°C. D1 + 0.1 M sorbitol (optional) : DMEM low glucose + 1% v/v HI-FBS + 0.1 M sorbitol In our hands, ...window) ). To prepare D1 + 0.1 M sorbitol, add 57 mL of 1.46 M sorbitol, 7.5 mL HI-FBS, and 7.5 mL 100X... of other components, such as sorbitol and sodium bicarbonate, sorbitol has been found to increase viral...filter clogging during harvesting. The addition of sorbitol has been shown to increase viral titers by up ... up to 1.8-fold. See the recipe for D1 + 0.1 M sorbitol above. Carefully pour off the media into a waste...

  13. Kit Free RNA Extraction

    Type
    Protocol
    ...collect as much of the top aqueous phase without disturbing the interphase layer, consider using a lower ... much of the ethanol wash as possible without disturbing the pellet. Air-dry the pellet for 5-10 minutes...

  14. Synthetic Biology - Overview

    Type
    Collection
    ... Leonard Han Lim Wendell Lim Timothy Lu Michel Maharbiz Thorsten Mascher Kathleen Matthews Anastasios ...

  15. Microbiology Resources

    Type
    Collection
    ...Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning and Expression Bacteria and yeast expression...

  16. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...information! Link in bio. #STEM #antibodies #molecularbiology #science #scicomm ♬ original sound - Addgene...

  18. What's New in CRISPR - May 2019

    Type
    Blog Post
    ... the Cas9-PCV and PCV-Cas9 plasmids Selective curbing of unwanted RNA editing (SECURE) base editors Keith...
Showing: 1 - 20 of 51 results