AAV Production in HEK293 Cells
Type
Protocol
...°C. D1 + 0.1 M sorbitol (optional) : DMEM low glucose + 1% v/v HI-FBS + 0.1 M sorbitol In our hands, ...window) ). To prepare D1 + 0.1 M sorbitol, add 57 mL of 1.46 M sorbitol, 7.5 mL HI-FBS, and 7.5 mL 100X... of other components, such as sorbitol and sodium bicarbonate, sorbitol has been found to increase viral...filter clogging during harvesting. The addition of sorbitol has been shown to increase viral titers by up ... up to 1.8-fold. See the recipe for D1 + 0.1 M sorbitol above. Carefully pour off the media into a waste...