We narrowed to 3 results for: rbis

1. AAV Production in HEK293 Cells

   Type

   Protocol

   ...°C. D1 + 0.1 M sorbitol (optional) : DMEM low glucose + 1% v/v HI-FBS + 0.1 M sorbitol In our hands, ...window) ). To prepare D1 + 0.1 M sorbitol, add 57 mL of 1.46 M sorbitol, 7.5 mL HI-FBS, and 7.5 mL 100X... of other components, such as sorbitol and sodium bicarbonate, sorbitol has been found to increase viral...filter clogging during harvesting. The addition of sorbitol has been shown to increase viral titers by up ... up to 1.8-fold. See the recipe for D1 + 0.1 M sorbitol above. Carefully pour off the media into a waste...

2. Kit Free RNA Extraction

   Type

   Protocol

   ...collect as much of the top aqueous phase without disturbing the interphase layer, consider using a lower ... much of the ethanol wash as possible without disturbing the pellet. Air-dry the pellet for 5-10 minutes...

3. AAV ddPCR Titration

   Type

   Protocol

   ... tubes to the BSC used for dilutions. Without disturbing the liquid in the tube, gently uncap the PCR ... tubes to the droplet generation BSC. Without disturbing the liquid in the tube, gently uncap the tubes...