...vein or retro-orbitally Gauge: 27–30 Volume: < 0.2 ml Skill required: beginner (retro-orbital) to intermediate...anesthesia Tail vein or retro-orbital 27–30 < 0.2 mL Beginner (retro-orbital) to intermediate (tail vein...injections.” However, they can also be given retro-orbitally, behind the eye. IV injections are used for a ... the correct vein without collapsing it. Retro-orbital injections are also thought to be less stressful...routes of administration in Mice-Percutaneous Retro-Orbital, jugular catheter, tail vein and femoral cut down...

...stopped talking to each other if I was not there to arbitrate for them. They were different people with some...leave everyone feeling vaguely unsatisfied. In arbitration, there is more of a winner and loser–one party...considered in the right, and one in the wrong. If you arbitrate, one party might get exactly what they want, but...punished or invalidated. Both compromise and arbitration might remedy short-term substantive conflicts...

...renewable biotechnology products and biosensors for herbicides as well as developing yeast as source for dietary...pesticides may be avoided or decreased. Biosensors for herbicide detection Dr. Rodrigo Arturo Rivera Solís, Universidad...thrives to make affordable biosensors that detect herbicides, such as glyphosate, in water. He aims to leverage...

...guest blogger Jon Backstrom, a biochemist in the Vanderbilt Eye Institute and Tonia Rex's lab. A common strategy...Backstrom. Jon Backstrom, Ph.D is a biochemist in the Vanderbilt Eye Institute and is particularly interested ...

...transformation fall into two main categories: antibiotic/herbicide resistance and fluorescent proteins. Selection...field conditions or in sterile conditions where disturbing the tissue for imaging or collection could contaminate...

... can be adapted to other sugars such as sorbitol. As sorbitol is already a common excipient in AAV formulation...

...requires constant turbidity of the culture, so bacteria must be grown in a turbidostat. Only mutate particular...

...efficiency when delivering these viruses via retro-orbital vs. tail vein systemic injections? A: We have not...difference in AAV transduction efficiency between retro-orbital versus tail vein injections.   Q10: Have you or...

...resistance to the herbicide chlorsulfuron (Homo line 2) or wild type (WT), two weeks after herbicide was applied...

...example, the Rainbow Vectors from the Mariette Barbier Lab can be used to fluorescently label a wide variety...Cybernetics. Colocalization of Fluorescent Probes. 7. Barbier, Mariette, and F. Heath Damron. "Rainbow Vectors...

...Thompson Over the last few years, there's been a disturbing trend in the number of high profile people denying...Ph.D., is currently a post-doctoral fellow at Vanderbilt University. She studies beta-cell function and...

...°C. D1 + 0.1 M sorbitol (optional) : DMEM low glucose + 1% v/v HI-FBS + 0.1 M sorbitol In our hands, ...window) ). To prepare D1 + 0.1 M sorbitol, add 57 mL of 1.46 M sorbitol, 7.5 mL HI-FBS, and 7.5 mL 100X... of other components, such as sorbitol and sodium bicarbonate, sorbitol has been found to increase viral...filter clogging during harvesting. The addition of sorbitol has been shown to increase viral titers by up ... up to 1.8-fold. See the recipe for D1 + 0.1 M sorbitol above. Carefully pour off the media into a waste...

...collect as much of the top aqueous phase without disturbing the interphase layer, consider using a lower ... much of the ethanol wash as possible without disturbing the pellet. Air-dry the pellet for 5-10 minutes...

... Leonard Han Lim Wendell Lim Timothy Lu Michel Maharbiz Thorsten Mascher Kathleen Matthews Anastasios ...

...Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning and Expression Bacteria and yeast expression...

...information! Link in bio. #STEM #antibodies #molecularbiology #science #scicomm ♬ original sound - Addgene...

...Cancer is the leading cause of cancer related morbidity and death among females in Ghana and in other ...

... the Cas9-PCV and PCV-Cas9 plasmids Selective curbing of unwanted RNA editing (SECURE) base editors Keith...

...like other endonucleases except that they cut at arbitrary sequences a defined distance away from the recognition...

...Bacterial Fluorescence Labeling Plasmids from the Barbier lab). Bright and early on Saturday morning, John...