...CRISPR-associated transposases (CASTs) combine the large-scale capacity of transposases (Tns) with the precise...Find plasmids for CRISPR-associated transposases (CASTs) for insertion of donor DNA. CRISPR...CRISPR Plasmids: CRISPR Transposases (CASTs) Browse CRISPR Plasmids By Function Genome Editing Cut Base...lacks nuclease activity, instead recruiting the transposase (comprised of multiple components) to target ...locations in the genome defined by a guide RNA. The transposase can then integrate donor DNA. Editing by CASTs... c omplex for a ntiviral de fense), and four transposase components (TnsA, TnsB, TnsC, and TniQ). Type...use a type V-K Cas effector, Cas12k, and three transposase components (TnsB, TnsC, and TniQ). Unlike type...

...of sample types (Engvall 1971). Scientists value ELISAs for their accuracy, specificity, and sensitivity...considerations when using the method. ELISA basics All ELISAs follow the same overall process. First users coat...figure was created with BioRender.com  Direct ELISAs A direct ELISA is the quickest and simplest of ...saves time, it also has several drawbacks. Direct ELISAs generally have higher levels of background staining...components and increase background staining. Direct ELISAs also lack the signal amplification afforded by ...research before conjugating at the bench.   Indirect ELISAs The indirect ELISA process is similar to that of...also be a source of cross-reactivity.  Sandwich ELISAs In the most common type of ELISA, the sandwich ...

...elements. By Ppgardne at English Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid...transcript. By Arunreginald at en.wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid...

.../d and the S. aureus Cas9 (PAM: NNGRRT) to create Sa-ABE8.x-m/d. With these variants, ABE8 could target...ABE7.10, NG-ABE8.xm/d showed a 1.6x median increase and Sa-ABE8.x-m/d a 2x median increase in editing frequency...

...References and resources References Biering SB,  Sarnik SA, Wang E, Zengel JR, Sathyan V, Nguyenla X, Van Dis...DM, Puschnik AS, Bertozzi CR, Carette JE, Stanley SA, Harris E, Konermann S, Hsu PD (2021) Genome-wide...

...doi.org/10.1186/s12859-017-1793-7 Dresios J, Chappell SA, Zhou W, Mauro VP (2005) An mRNA-rRNA base-pairing.../doi.org/10.1101/gr.139758.112 Mauro VP, Chappell SA (2014) A critical analysis of codon optimization ...

...pyogenes (Sp) 3' NGG Staphylococcus aureus (Sa) NGRRT or NGRRN Neisseria meningitidis (Nm ...K, Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically...XD, Liu P, Edraki A, Mir A, Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern...

...Arakaki J, Fuqua MA, Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, Rafelski SM, Gunawardane...

...Staphylococcus aureus Cas9 (Sa-Cas) and deposited plasmid IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1 ...

...27096365 Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. 2015. Multicolor CRISPR labeling...Makarova KS, Wolf YI, Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou R, Brouns SJ, Charpentier...

...human cells. Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci U S A. 2015...

...5:11771. doi: 10.1038/srep11771. PubMed. McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL....

...Find the Tet-inducible caspex plasmid here. Myers SA et al. Nature Methods. 2018. PMID: 29735997 bioRxiv...

...PowerPoint etc) or Programming skills (Python, R, SAS, HTML, Matlab etc) are highly desirable in a range...

...or GAT SpCas9-NG 3' NG  Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N)  Acidaminococcus...

... of paraspeckles. Clemson CM, Hutchinson JN, Sara SA, Ensminger AW, Fox AH, Chess A, Lawrence JB Mol Cell...

...molecular neuroscience. 2014 Nov 18;7:88. Tian L, Hires SA, Looger LL. Imaging neuronal activity with genetically...

...Mintseris J, Mohler K, Levy T, Aguiar M, Beausoleil SA, Paulo JA, Rinehart J, Huttlin EL, Gygi SP. Nat Methods...

...33246-4 Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper RK, Tsien RY, Lin MZ. Tunable and reversible...

...Techniques References Bell AD, Usher CL, McCarroll SA. Analyzing Copy Number Variation with Droplet Digital...