We narrowed to 819 results for: ust

...payment, and billing. Industry Material Transfer Agreements Most Addgene orders must be accompanied by a...growing collection of plasmids that are available to industry. Learn more about the available plasmids and ... Distribution to Industry Featured Collections Search Plasmids Plasmids Kits Pooled...Addgene's Search to find plasmids available to industry scientists while also adding additional filters...of the plasmids that are currently available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit...and quality control by Addgene as well as by our trusted partner labs. Learn More ID Recombinant Antibody...us know what you would like to see available to industry scientists. Please note this does not guarantee...

...AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry postdoc in neuroscience at...talked to people with industry experience, the more I heard that getting into industry early would only help...to postdocs in both industry and academia. Pros and cons: The academic vs. industry postdoc  Time: Academic..., many believe that industry postdocs, having already been immersed in an industry environment, have a...biotech. In other words, industry postdocs already have the prerequisite “industry experience” that I’ve ...as a requirement on some industry job postings. Also, if you work in industry for any length of time, ...be higher in an industry setting. Scientific community and mentorship: As an industry postdoc, you might...

...fluorescently labeled piggyBac vectors for uniform and sustained expression of single or multiplexed gRNAs and ...

...Congratulations, you’ve just landed an interview for an industry job! You’ve worked hard to earn this...experience not long ago. I just recently started working in the pharmaceuticals industry after earning my Master...jobs, I interviewed with just the PI of the lab and for one position, I just had a phone interview. I ...you feel a little panicked. You’ve never had an industry interview before. What should you do to get ready...between applying for an academic position versus an industry position is the on-site interviewing process. ...groups without having a formal interview process. Industry interviews, however, were more structured. I met...is what I’ve found helpful for my interviews in industry. Prepare stories to tell in your interview The...

...had quite the up-tick in views over the month of August - at over 56,000 views, we’ve grown 12% over last...surprising given how many scientists go on vacation in August (for another part of my job, I travel around the...posts as each one has something great to offer, I just need to get back to emailing (hopefully non-vacationing...non-vacationing) scientists. You can find all of our August posts in chronological order here: Site Directed...

... the second in a series of posts on bringing sustainable practices in the lab. The first post focused ...learn about some creative approaches to lab sustainability, and find ways to further reduce our consumption...consumption beyond recycling.     Choosing more sustainable products Recycling is great for rerouting waste... grant agencies), we can shift toward a more sustainable one. The math on this is pretty simple: wash ...grades of plastic lumber and distribute it to industrial and residential markets. Rethinking our approach...

...more lab sustainability tips, check out our other articles in this series: Bringing Sustainable Practices... specialized companies can recycle plastic-- you just have to find them (or keep reading). Corning takes...Encourage your workplace to become a leader in sustainability. Sign up for blog posts and updates from ...individuals, companies, and universities to be sustainability leaders. Take initiative to bring ideas to ... A lot of places have small or non-existent sustainability offices, so it’s up to all of us to work on...Practices to the Lab: Innovations Bringing Sustainable Practices to the Lab: Easy Action Items References...

...Luckily, the Green Listed software can help you do just this, probably in less than a minute (1). Green...is a new software tool used to design gRNAs for custom CRISPR screens targeting a (long or short) list...Glance!  CRISPR knockout screens: Full genome vs. custom screens Full genome CRISPR knockout screens have... being involved in the studied phenotype. For robustness of a screen, each gene is not targeted by only...hypothesis driven research question. Examples of custom CRISPR screens Differentially expressed genes  ...massive amount of descriptive transcriptomics data. Custom CRISPR screens designed to target differentially...microbe or a cytokine is added to a cell). With a custom CRISPR screen and the appropriate phenotypic readout...

... and increase your lab sustainability. For my other posts on lab sustainability, check out my Recycling...This is the last post in a blog series on sustainable practices in the lab, where I’ll list some easy...unimportant. With so many big contributors to the sustainability problem, how can one little change make a difference...quick things above, try joining or starting a sustainability project. This could be: Setting up a recycling...Learn about other creative approaches for lab sustainability Resources on Addgene.org Sign up for our ...

...Addgene’s customer service philosophy Customer service can be a hassle. You finally make it through a...of our core values here is to provide unmatched customer support, as this is vital to ensuring that our...technical, or quality control issues. Improving customer support with self service We strive to make our... at the moment you’re looking for it, or you may just prefer to troubleshoot yourself without contacting...waiting for an answer. Luckily, there’s a trend in customer support to address this exact problem, and it’... find the answer that you’re looking for, or you just have a general question or concern about the answers... customer support...

...from.   Acoustic reporter genes (ARGs) Acoustic Reporter Genes (ARGs) are engineered gene clusters that ...optimized for expression in E. coli: acoustic gene 1 (arg1) and acoustic gene 2 (arg2). The key difference...Bourdeau et al calls this method acoustic collapse.  Importantly, acoustic collapse doesn’t affect bacterial...for serial acoustic collapse to aid in the development of ARG constructs with new acoustic phenotypes....but each of these reporters has its drawbacks. Acoustic reporter genes (ARGs) overcome these limitations...possible if bacteria are sequentially exposed to acoustic pulses with high enough amplitudes to first collapse...viability and with 20 hours of growth following acoustic collapse E. coli re-express arg1 gas vesicles ...

...What's a lab group in industry? The collaborative nature of working in industry and how delegating can...Crystal’s exciting career path, to gain insight into industry research, and to get advice on how to succeed ...importance of thinking about the big picture and not just the next experiment. Chapter 6: Advice to scientists...scientists who are planning on working in industry. The importance of recognizing and leveraging your unique...adapting to needs as they arise. Tips on applying for industry jobs and deciphering job listings. Mobility between...

...What are Acoustic Reporter Genes (ARGs)? Fluorescent and bioluminescent reporter genes are widely used...members of our lab - the Shapiro Lab - developed Acoustic Reporter Genes (ARGs), which are sets of genes...inside the body. So we screened gas vesicles gene clusters from a diverse set of gas vesicle-expressing species...39006 produced the highest levels of nonlinear acoustic contrast in E. coli. We deleted an unannotated...the first-generation bARGs, poor expression and acoustic properties of these gas vesicles made non-destructive...expression, a smaller genetic footprint, and nonlinear acoustic scattering. The best candidate for this was the...the Anabaena flos-aquae gas vesicle gene cluster because of its small size and its ability to produce ...

...available to industry scientists  While we’ve focused on expanding COVID-19 plasmids to industry scientists...available to industry scientists To make plasmid sharing more open for academic and industry scientists,...available more broadly: to scientists in academia and industry. As a result over 90% of Addgene’s COVID-19 resources... to Addgene’s new approach of working with the industry-based scientific community. Conversations with...Academy of Medical Sciences University of Texas at Austin University of California, San Francisco  University...to the scientific community - both academic and industry scientists. This partnership complemented each...we have other plasmid collections available to industry? Over the past 8 years, several organizations ...

... et al., Neuron. 2019. PubMed PMID: 30704911. Acoustic reporter genes for noninvasive imaging of microbes...Article contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment In recent years,...solution, Mikhail Shapiro’s lab has developed four acoustic reporter gene (ARG) plasmids to better monitor...the ARG plasmids at Addgene. For more details on acoustic reporters, check out this blog post. Bourdeau ...differentiation. Furthermore, TALEN and ZFN require custom nucleases for each gene of interest. CRISPR-Cas9...

...Backbones Plasmid Description Industry Return to top Blue Plasmid Description Industry Return to top Cyan Plasmid...Plasmid Description Industry Return to top Green Plasmid Description Industry Return to top mEmerald Plasmid...Plasmid Description Industry Return to top Yellow Plasmid Description Industry Return to top Orange Plasmid...Plasmid Description Industry Return to top Red Plasmid Description Industry Return to top Photoactivatable...interest. Most of these plasmids are available to industry scientists as well as academic and nonprofit labs...photoactivatable fluorescent proteins. Plasmid Description Industry Return to top Content last reviewed: 25 September...

.... You must reply to that email within 10 business days in order to proceed with any adjustments to the.... You must reply to this email within 10 business days in order to proceed with any adjustments to the... for any additional customs fees or taxes, and to complete any additional customs documentation that might...research materials. Please be aware that additional customs charges may be required before your order will ...

... have some restrictions (for example, the target must start with a T), but they still have potential targetable... spring of 2011, the groups describe a set of customized plasmids that can be used with the recently developed...plasmids, which are available as a kit from Addgene , custom arrays consisting of 12-31 repeats can be assembled...assembled and inserted into a variety of backbones in just a few steps ( Figure 2 ). The Bogdanove group also...kit, first described in Nature Biotechnology in August, uses a serial ligation protocol to assemble arrays...here . References Efficient design and assembly of custom TALEN and other TAL effector-based constructs for...697-8. Pubmed PMID: 21822241 . TAL effectors: customizable proteins for DNA targeting Bogdanove AJ, Voytas...

...available to industry, in addition to academics and nonprofits, and this is denoted in the industry column ...our Plasmid Distribution to Industry page. ID Plasmid Description Industry PI Additional Addgene resources... MERS, BAT-CoV Plasmids ID Plasmid Description Industry PI Due to export control regulations, MERS and...related to these genes below. ID Plasmid Description Industry PI Additional Addgene resources ACE2 plasmids ...nanobody plasmids And more! ID Plasmid Description Industry PI Addgene blog Finding nucleic acids with SHERLOCK...

...Editing : pegRNAs must serve as both a guide and a template. In general, edits must be downstream (3’)...Delivery Validate Genetic Edit References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R ... is less efficient Each putative knockout allele must be experimentally verified Cut Nick Homology Directed...edit. Lastly, you will need to know if your edit just affects the genomic sequence or if it also affects... targeted edits can lead to changes in more than just the DNA sequence. If this is the case, you can confirm...paired with restriction enzymes for further customization Usually paired with a sequencing method Can’...