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We narrowed to 819 results for: cell

Showing: 91 - 100 of 819 results

  1. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...). These cell-permeable Janelia Fluor (JF) dyes have excellent properties inside living cells, especially... fluorescent proteins. In-cell labeling strategies The majority of in-cell labeling strategies have two...and oligonucleotide labels for fixed-cell imaging, not for live-cell applications. Thus, these fluorophores..."Imaging live-cell dynamics and structure at the single-molecule level." Molecular cell 58.4 (2015): 644...sparked a renaissance in biological imaging. Suddenly, cell biologists were no longer beholden to chemists and.... Add a dash of DNA with an electrical jolt and cells become perfectly capable of synthesizing fluorophore...question: In this age of GFP and its ilk, why should cell biologists talk to chemists? One reason is something...

  2. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...enzyme-mediated proximity cell labeling strategy to detect cell-cell interactions in vivo (Ge et al., 2019...about RapID! EXCELL: Proximity labeling for the detection of cell-cell interactions EXCELL is an enzyme-mediated...et al. "Enzyme-Mediated Intercellular Proximity Labeling for Detecting Cell–Cell Interactions." Journal...a bait protein fused to BirA* in cells and then incubate those cells with exogenous biotin. After a few...-protein interactions, and even more recently cell-cell interactions. If you’re looking to identify interactions...proximal and interacting proteins in mammalian cells." J Cell Biol 196.6 (2012): 801-810. PubMed PMID: 22412018...peptide (LPETG). In EXCELL, the mgSrtA variant can be displayed on the surface of a cell of interest, where...

  3. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...restoring mCherry expression. Cells can then be sorted using Fluorescence-activated cell sorting (FACS) and the...easily determined by comparing cells expressing only eGFP expression to cells expressing eGFP and mCherry...expressed in cells. mCherry is constitutively expressed to identify successfully transfected cells. C-to-T ...eGFP and mCherry positive cells by the number of only mCherry positive cells (Martin et al., 2019). ...TREE) to purify single base edited cells without the need for single cell isolation and downstream sequencing...fluorescence and cell sorting using FACS which could limit their use across different cell types and systems...sgRNA targeting the GFPACG to the cells. As expected, only the cells that contained the cytidine base ...

  4. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

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    Blog Post
    ...engineered cells in therapy. The lungs are difficult, but other tissues like the retina have cell-based therapies...Chapter 9: Using stem cells as in-vitro models of disease. How Darrell is using stem cells from patients to...sharing reagents, cell lines, data, and knowledge without exclusivity. Chapter 11: The Stem Cell Bank (CReM ...the lungs, and, among other projects, using stem cells to develop in vitro models for lung disease. Darrell...what drives him as a praticing physician and stem cell researcher. Darrell's ultimate goal - to develop...means to treat disease through tissue repair, stem cells, gene therapy, and tissue engineering. How the multidisciplinary...projects - differentiating surfactant producing cells that can be used to better understand the biology...

  5. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    Blog Post
    ...transfected cells, we count the number of tag-positive cells (tag ICC) as a percentage of total cells in the...Deliver csRNP into cells: Prepare cells for transfection, add csRNP and ssODN to the cell suspension, transfect...csRNP plus ssODN, is then transfected into stem cells. Cells are allowed to recover for 2-5 days and then...efficiently these components are delivered into cells; for each cell type, the transfection/delivery step needs...Proteins - the workhorses of the cell – never work alone in the cellular milieu. It is, therefore, critical...DNA) to perform diverse biochemical tasks in the cell. One of the most popular approaches to study protein...with its associated protein complexes - from the cell-lysate using antibodies against the target protein...

  6. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post
    ...unstable, meaning that the cells must continually produce antitoxin to avoid cell death. In the case of plasmids...addiction systems” require dividing cells to retain plasmids - if a cell does not inherit plasmid due to ...will be degraded leaving the cell to be killed by the more stable toxin. Cells only survive if they retain...10% of cells while plasmids containing the plasmid addiction system were retained in 75% of cells. They...designated this fragment as the ccdB region (for “coupled cell division”). The encoded toxin-antitoxin system consists...cleaved complex. This results in DNA breakage and cell death. Conversely, CcdA binds to and blocks the ... replaced by the gene of interest. As a result, cells that retain the ccdB gene die off and a higher percentage...

  7. Plasmids 101: Control Plasmids

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    Blog Post
    ...untreated cells—but can we be certain? Do we know whether the plasmid delivered into the cells successfully...amount of DNA transfected into the cells and the ability of the cells to express the protein.   In analysis...Gene X in backbone Y) was transfected into human cells. From this result one could simply conclude that...didn’t work, as the expression level of Gene X in cells treated with Plasmid A is similar to the expression...or transfection process) may have on the target cells. One transfection control is an empty vector control... itself has any cytotoxic effects on the target cells. Another type of transfection control is an internal...plasmid or transfected into a separate well of your cells. Regardless, the amount of reporter protein activity...

  8. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...lines expressing GFP in specific cell types to do more than just label cells. If GFP could be co-opted to ...reporter output was observed in their 293 cell culture system. In cells cotransfected with GFP and the GBPs,...expression was induced in ~76% of GFP+ cells, and 100% of DsRed+ cells also expressed GFP. Using AAV constructs...fluorescent proteins as scaffolds for cell-specific gene manipulation.” Cell 154(4) (2013): 928-939. PubMed ...wanted to express a variety of proteins in single cell types in the mouse, but knew it would take years...could then selectively manipulate only GFP-labeled cells. Once Tang and Cepko found a description of GFP-...heavy chain, and they are small and stable inside cells. Nanobodies binding GFP were designed in 2009. Subsequent...

  9. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...the producer cells and purification protocols often describe a process that begins with cell lysis to release... steps to separate cell culture supernatant containing exo-AVVs from producer cells, and then to enrich...effectively target less accessible cells, such as brain tumor stromal cells in a mouse model of glioblastoma...particles is able to infect more cells (as demonstrated in different cellular environments such as retina ...Exosomal vesicles secreted from producer cells also contain cellular macromolecules (e.g. proteins, nucleic...Standard AAV vectors are generated with a producer cell line like the widely used 293T. During vector production... to be spontaneously released from the producer cells into the supernatant during the production phase...

  10. Hot Plasmids and Viral Preps - September 2021

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    Blog Post
    ...improved cell-free system to examine biosynthetic pathways A new tool for enriching ABE-targeted cells The ...interest, and cell extract.    Graphical Abstract: Schematic representation of cell-free system,...successfully reconstituted in this cell-free system. In summary, this cell free system allows for a relatively...multiplexed and can be used in human pluripotent stem cells, a cell type that has been difficult to modify in the... Corner New ready-to-use viral preps   Improved cell-free transcription/translation system to examine...biosynthetic pathways  By Gabrielle Clouse Recently, cell-free transcription/translation systems (also referred....0c00581   New tool for enrichment of ABE-targeted cells By Ellie Adams Single base modifications through...
Showing: 91 - 100 of 819 results