...When a dish of mammalian cells is transiently transfected with a plasmid containing a fluorescent protein...

...PubMed PMID: 26914317. 4. Mock, U. et al. mRNA transfection of a novel TAL effector nuclease (TALEN) facilitates...

...laboratories. The development of the transient transfection platform for assaying transcriptional activity...

... Vector Core (Link opens in a new window) . Transfections are performed using the transfer plasmid, a ...

...single gene. The gRNA expressing cells were then transfected with a Cas9-expressing plasmid to generate a ...luciferase. The bait and prey plasmids are co-transfected and proteins are immunoprecipitated from the ... plasmid is also available). Second, they co-transfected short-arm donor vectors containing mAID and either...efficiently creating stable cell lines using direct transfection precluding the need for virus. Furthermore, ...

...bonds, monobodies can be easily expressed in transfected eukaryotic cells, allowing for them to be used...

...hippocampal cultured neurons, imaged 14 days post-transfection, excited at 505 nm (right). Scalebar represents...

... include both scrambled RNA motifs and cells transfected with BirA* protein alone (i.e. no RNA bait). ...

... plasmid). Viral production then proceeds by transfection of all three plasmids into a producer cell line...

...targeting experiments allows you to visualize transfection efficiency and even track localization in cellular...