We narrowed to 144 results for: transfection

...pSpCas9(BB)). The 2A-GFP allows for screening of transfected cells. Find pSpCas9(BB)-2A-GFP (PX458). lentiCRISPR...

...laboratories. The development of the transient transfection platform for assaying transcriptional activity...

...single gene. The gRNA expressing cells were then transfected with a Cas9-expressing plasmid to generate a ...luciferase. The bait and prey plasmids are co-transfected and proteins are immunoprecipitated from the ... plasmid is also available). Second, they co-transfected short-arm donor vectors containing mAID and either...efficiently creating stable cell lines using direct transfection precluding the need for virus. Furthermore, ...

...bonds, monobodies can be easily expressed in transfected eukaryotic cells, allowing for them to be used...

...hippocampal cultured neurons, imaged 14 days post-transfection, excited at 505 nm (right). Scalebar represents...

... include both scrambled RNA motifs and cells transfected with BirA* protein alone (i.e. no RNA bait). ...

... plasmid). Viral production then proceeds by transfection of all three plasmids into a producer cell line...

...cells in CS2 Day 2: Seed cells in CS5 Day 3 (am): Transfect cells Day 7 (am): Harvest cells Equipment Class...brands and lots of FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to...Polyethylenimine (PEI) 25 kDa MW Pro-Tip Other transfection reagents may be used in this protocol, but their...their conditions must be optimized. Plasmids for transfection: pHelper; pRC (Rep-Cap), plasmid expressing ... Pass and plate the cells the day before the transfection. Thaw a new vial of cells after 30 passages....to the incubator for 24 h–36 h. Proceed with transfection: Calculate the amount of each plasmid needed...Note that longer incubation times can reduce transfection efficiency. Add the OptiMEM + DNA + PEI solution...

...Transformation, Transduction, Bacterial Conjugation, and Transfection Resources on Addgene.org Addgene's Synthetic...

...over for other applications, like PCR, cloning, transfection, or long-term storage. But what about those ...