Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 6 results for: transfection

Showing: 1 - 6 of 6 results

  1. Plan Your Experiment

    Type
    Guide
    ...efficiently transfect. However, note that this method is most useful for cell lines that you can transfect at ...CRISPR system into mammalian cells is to directly transfect expression plasmids encoding your Cas protein ...gRNA, which you generally perform by chemical transfection or electroporation. Expression can be transient...Lentiviral vectors are a good option for difficult-to-transfect cells. You can direct expression to specific cell...These methods are more transient than plasmid transfection or viral vectors, reducing the risk of accumulating...method is helpful in cells that are difficult to transfect, transduce, or may not express common promoters...

  2. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...transfer, packaging, and helper plasmid) are transfected into cells which then act as in vitro factories...rare possibility that recombination between the transfected plasmids could lead to the production of replication-competent... AAV Vector topics Addgene Protocols General Transfection AAV Production in HEK293T Cells AAV Purification...and E1B19K genes provide a helper function for transfection-based adeno-associated virus vector production...DNA into eukaryotic cells using viral vectors. Transfection The process of artificially introducing foreign...

  3. Adenovirus Guide

    Type
    Guide
    ...rare possibility that recombination between the transfected adenoviral transfer plasmid and the E1 region...HEK293 or HER911 cell lines expressing E1 are transfected with the linearized construct, and rAdV vectors...packaging space. However, these constructs must be transfected into HER911 E4+ cells for rAdV vector production...DNA into eukaryotic cells using viral methods. Transfection The process of artificially introducing foreign...

  4. Lentiviral Vector Guide

    Type
    Guide
    ...three or four) are transfected into HEK293T cells using your preferred transfection method. The HEK293T... third-generation) can be used in transient transfections, but expression will be greatly limited due ...lentiviral plasmids cannot be used in direct transfections for transient expression of a transgene. For...

  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...above (envelope, packaging, and transfer) are co-transfected into the HEK293T packaging cell line. This system... number of plasmids that are required in the transfection step of the viral packaging cell line. For example...

  6. Antibody Guide

    Type
    Guide
    ...hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express antibodies....
Showing: 1 - 6 of 6 results