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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 20 results for: transfection

Showing: 1 - 20 of 20 results

  1. CRISPR Plasmids - Drosophila

    Type
    Collection
    ... BspQI Transfection Puromycin yes, cut Ji-Long Liu 45946 pU6-BbsI-chiRNA dU6 BbsI Transfection none, need...Bullock and Port 51026 U6-BbsI-crRNA dU6 BbsI Transfection none, need Cas9 plasmid O'Connor-Giles , Harrison...

  2. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...another transfection method such as cationic liposome-based transfection. Optimize transfection conditions...Perform maxi-prep for each CRISPR/Cas9 construct. Transfecting CRISPRs into Cells of Interest NOTE: This protocol...which is used for MEL cells. However, transient transfection of CRISPR/Cas9 plasmids may be successfully ...types using preferred culture conditions and transfection strategies for each cell type. While MEL cells...Fluorescence Activated Cell Sorting (FACS) of Transfected Cells Prepare cells for FACS by filtering them...

  3. TALEN Plasmids and Kits

    Type
    Collection
    ...Gate-assembled TALEN, both in microinjected embryos and transfected cells. TALEN expression is driven by the strong...expression in mammalian systems including hard-to-transfect cell lines. Features include (i) Esp3I (BsmBI)...on pTAL7a and pTAL7b for enrichment of double-transfected mammalian cells. In addition, a T7 promoter was...alternative. Based on transient double-selection of transfected cells, the system has successfully been employed...

  4. DNA Service - Cloning Grade DNA

    Type
    Collection
    ... different DNA concentrations. Can I directly transfect cloning grade DNA into cells? Cloning grade DNA...designed for use in cloning applications. Direct transfection into cells is not recommended. Can I order more...

  5. Plan Your Experiment

    Type
    Collection
    ...efficiently transfect. However, note that this method is most useful for cell lines that you can transfect at ...CRISPR system into mammalian cells is to directly transfect expression plasmids encoding your Cas protein ...gRNA, which you generally perform by chemical transfection or electroporation. Expression can be transient...Lentiviral vectors are a good option for difficult-to-transfect cells. You can direct expression to specific cell...These methods are more transient than plasmid transfection or viral vectors, reducing the risk of accumulating...method is helpful in cells that are difficult to transfect, transduce, or may not express common promoters...

  6. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...(see paper) Transfection none, need Cas9 plasmid Calarco 47943 pMB70 cU6 BsaI Transfection none, need ...

  7. Antibody Production

    Type
    Collection
    ...expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding the heavy and light...contain low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified... The fluorescence pattern is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections...antibody. Protein expression is compared to an untransfected control and the protein size is confirmed. Residual...

  8. Luciferase Plasmid Collection

    Type
    Collection
    ...constitutive promoter can be used to monitor transfection or viral infection efficiency, to study changes... Renilla luciferase is used to monitor viral transfection Feng Zhang 21471 pLenti PGK V5-LUC Neo (w623... expressing firefly luc for monitoring plant transfection. Daniel Voytas 133312 pMKV057 Firefly AtUbi10...and IPT, with firefly luc for monitoring plant transfection. Daniel Voytas 181934 pC0037 Gaussia, Cypridina...

  9. Zhang Lab CRISPR Page

    Type
    Collection
    ...human codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols... #61422 - #61424 were designed for transient transfection : 61422 : Expresses dCAS9-VP64 activator with...codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons using a protocol similar to ...

  10. Mammalian RNAi Tools

    Type
    Collection
    ...short interfering RNA). Delivery can occur via transfection or viral delivery of a plasmid. shRNAs are often...

  11. Adenovirus Plasmids

    Type
    Collection
    ...both plasmids. This DNA molecule can then be transfected into mammalian packaging cell lines to generate...

  12. Antibody Plasmid Collection

    Type
    Collection
    ...Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells, the R-mAbs...

  14. Brain Initiative Collection

    Type
    Collection
    ...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s...Synapsin1 promoter, for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2...

  15. Viral Vectors

    Type
    Collection
    ...the delivery of genetic information to hard-to-transfect mammalian cells. In addition, "viral pseudotyping...

  16. Viral Production

    Type
    Collection
    ...the University of Pennsylvania Vector Core. Transfections are performed using the transfer plasmid, a ...

  18. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...generation R-mAbs plasmids, production of R-mAbs in transfected cells and their validation can be found below...inexpensively generate R-mAbs by transiently transfecting the plasmids into mammalian tissue culture cells... plasmids can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target Target...following plasmids can be used to produce scFvs from transfected mammalian cells. Addgene ID Plasmid Hybridoma...plasmids can be used to produce nanobodies from transfected bacterial cells. Addgene ID Plasmid Hybridoma... recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells....

  20. Antibody Guide

    Type
    Collection
    ...hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express antibodies....
Showing: 1 - 20 of 20 results