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We narrowed to 1,010 results for: CAL;

Showing: 181 - 200 of 1010 results

  1. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...Miller-Rhodes from the University of Rochester Medical Center.  You’ve probably heard of Forster Resonance...used the method to interrogate the subcellular localization of interactions between basic leucine zipper...it requires can lead to aberrant subcellular localization of the studied PIPs. The straightforward nature...2018), the generation of such resources in less genetically tractable organisms lags behind. BiFC can also...to the N- and C-terminal end of each PIP and empirically test which combination provides the best fluorescent...counterparts using western blotting. The subcellular localization of BiFC constructs should also be compared against...example), as this can also be perturbed by supraphysiological expression levels. BiFC quantification This...

  2. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... tools for all-optical electrophysiology A major challenge in the field of all-optical electrophysiology...response to blue light, which will ultimately co-localize the VP64 transactivator with a dCas9 that has ...expression Brilliant technologies adopt basic biological concepts and employ them in creative and innovative...protein multimerization is seen in many important biological contexts, such as the enhancement of transcriptional... of a transcription factor to a promoter and localization of a protein via the presence of multiple copies...system occurs via antibody-peptide labeling; specifically, cognate peptide epitopes fused to the protein...fluorescent and luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation...

  3. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    ... patients, specifically children and patients with skin disorders for research and clinical trials, as...system thus provides a practical way to generate transgene-free, virus-free clinical grade hiPSCs from easily...22422317. 6. Diecke, S., et al., Recent technological updates and clinical applications of induced pluripotent...Their work opened up vast possibilities for the clinical and therapeutic applications of iPSCs. The generation...SOX2, KLF4, and C-MYC [1]. For the derivation of clinical grade hiPSCs, reprogramming methods that do not...therapy trials and remains a safety concern in the clinical field [4]. Three major reprogramming methods are... are considered when generating hiPSCs for clinical purposes: mRNA transfection- this allows transient...

  4. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...D) teams at pharma companies carry out preclinical and clinical research to discover and test the best...molecular behaviors of chemicals and proteins to learn how a particular drug or medical device works. This... consulting? Healthcare consultants advise pharmaceutical companies, biotech start-ups, health insurance...efficient ways of delivering healthcare. Clients typically engage consultants as short-term experts to work...different kinds of challenges that consultants get called upon to tackle, depending on the part of the company...acquisitions of or collaborations with other companies. Medical Affairs groups work on building awareness among...among physicians and scientists about clinical research, drugs, the diseases they can treat, and diagnostic...

  5. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...next logical step after technical mastery. However, being a great scientist doesn’t automatically translate...transition While a PhD equips you with exceptional technical skills, transitioning into management often means...stepping into unfamiliar territory. Unlike solving technical problems in the lab, management involves a different...time, I discovered that I not only enjoyed the technical aspects of my work but also found fulfilment in...your team's—can lead to frustration. Clinging to technical tasksDelegation can feel unnatural for those accustomed...myself to focus on my core responsibilities, which typically revolved around the broader vision rather than...insights, lessons from their experiences, and practical advice for overcoming challenges. Experimenting...

  6. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...revolves around synthetic humans called replicants which are genetically engineered by a powerful corporation...fused a catalytically impaired Cas9 protein to a DNA-editing enzyme to generate the prototypical base editor...STUDIES ON THE CHEMICAL NATURE OF THE SUBSTANCE INDUCING TRANSFORMATION OF PNEUMOCOCCAL TYPES. The Journal...Rallapalli, a graduate student at the University of California, San Diego. The revolution in genetic engineering...technology led to the development of many important medical and agricultural products. But it also made SciFi...break at the target genomic location. This is typically followed by non-homologous end-joining that results...sees the USS Enterprise crew fight against a genetically engineered superhuman Khan. The novel, Inferno...

  7. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...intramuscular (IM), intrathecal (IT), and intravenous delivery (IV). In mice, IV injections are typically administered...rather than being geographically constrained to the brain. In cases where it’s critical to minimize or eliminate...crossing into the brain. While helpful from a biological standpoint, it makes delivering AAVs to the brain...throughout the organism to target large or diffuse biological structures. Of primary interest here are systemic...peripheral nervous system (PNS). (Capsids, if you recall, are the outer shell of an AAV vector, which determine...Where do they come from? Systemic capsids are typically derived from AAV9, which is one of a handful of...capsid applications. For example, AAV-PHP.eB is typically administered at a total dose between 1 x 1011 ...

  8. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ... a slightly slower scale.  All living cells with a membrane possess an electrical state. This state, ...potential (Vmem), can be measured as the imbalance of electrical charge between the inside and the outside of ...bioelectricity is revealing the important role of bioelectrical signaling mediated by ion-channels and pumps...affect patterning and in some cases even override genetically encoded patterning. Based upon trends in studies...formation of whole eyes from cell types that don’t typically give rise to eyes (Pai et al., 2011).  Additionally...head and a tail, and when gap junction-mediated electrical communication is briefly inhibited in the entire... two-headed worm, despite the fact that it’s genetically wild type  (Oveido et al., 2010). One of the ...

  9. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...the nucleus, also called the 4D nucleome. Visualizing specific loci has historically been difficult, as...successfully resolved loci with physical map distances of 2 and 75 Mbp, with the calculated fluorescent distances...to label certain loci, but this approach is not scalable for every locus...unlike CRISPR. Early CRISPR ...Figure 1: CRISPRainbow was used to track the localization of 6 chromosome-specific loci in the U20S cancer... every genomic locus, and to do so in vivo. Catalytically dead Cas9 (dCas9) cannot induce gene editing... to a given genomic locus, you can track the localization and movement of this locus in live cells! This...distances correlating with the previously established physical map. In comparing pairs of targets ~2 Mbp apart...

  10. The time and cost required to make a plasmid

    Type
    Blog Post
    ...plates and gels, which either don’t scale or have limited scaling. The downside of high-throughput work...Designing precise calculations for any of the numbers in this post is a mathematical story well beyond... Addgene provides large-scale savings. How large? Using Doench’s calculations and the 2018 numbers for...Debate of ’18, Blue Flame depositor John Doench calculated the time to make a plasmid. Doench’s lab in the...time it takes to make a plasmid could increase dramatically if you are piecing together several PCR products...long does it take in total? While much harder to calculate, in both our 2018 and 2023 poll, most people said...much does it cost to make a plasmid? Doench also calculated the cost of consumable reagents used to make ...

  11. Evolution of Lab Techniques

    Type
    Blog Post
    ... brainstorming new technological innovations. Let’s take a look at three historical techniques: southern...computers, cell phones, and cars become more technologically advanced leaving earlier versions obsolete,...that save both time and money. Yet, knowledge of historical techniques comes in handy whether you are perusing...cloning or knockout experiments to forensics and clinical diagnostics. One example of southern blotting ...fragments of a DNA sequence of interest to create a physical map of the DNA. Starting in the 1970s, restriction...keep restriction enzymes on hand for cloning and analytical digests. A researcher can test the orientation...). Sanger sequencing uses modified nucleotides, called dideoxynucleotides (ddNTPs), lacking the 3’ hydroxyl...

  12. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...Sensitivity in Western Blotting. Journal of Pharmacological and Toxicological Methods, 64, 233–237. https://doi....to get right. If you’ve thoughtfully done the technical design of your blot, but are still finding yourself... be done at 4 °C or on ice, whichever is more practical. If your lysing method is not resulting in your...Most people will simply load an extra ladder or technical replicate (ie, leftover sample) to fill the relevant...you may want to consult Tables 1 and 2 in our Technical Design of a Western Blot post to make sure you...transfer. Troubleshooting The two most common (non-technical) issues with transferring are smaller proteins...than just high background, your issue is likely technical. White circles, for example, could indicate that...

  13. Typing CRISPR Systems

    Type
    Blog Post
    ...notable in recent years. A variant of V-U, paradoxically called V-K, is a CRISPR-associated transposase ... CRISPR-Cas systems. Philosophical Transactions of the Royal Society B Biological Sciences, 374(1772),...What’s in a type? That which we call CRISPR, by any other name would…probably still edit genomes. Understanding...function and composition; subtypes and variants are typically based on species of origin. There are currently...systems. Type III systems are unique, as they can technically target both RNA and DNA, though it is largely...systems function, a III-E effector was developed, called Cas7-11. Despite being in Class 1, Cas7-11 is a... that other CRISPR types do, due to a lack of canonical CRISPR features (Pinilla-Redondo et al., 2019;...

  14. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    ...UCIBIO) focused on understanding the physiological and pathological roles of human cell glycans. Accordingly... biosynthetic pathway and have a wide range of clinical manifestations. PMM2-CDG is the most frequent ...reported that PMM2-CDG patients suffer from immunological defects. These are characterized by a predisposition...these lines to study how immune processes and biological pathways are altered in PMM2-CDG. These studies...reducing their risks for infection and other pathological conditions. References: Monticelli, M., Ferro... J., dos Reis Ferreira, V. & Videira, P. A. Immunological aspects of congenital disorders of glycosylation...Biology at the University of Colorado Anschutz Medical Campus in Aurora, Colorado, USA. The primary focus...

  15. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...signal is in hot and the anatomical B-mode images are in greyscale. (scalebar = 2mm)   We hope you...the tumors was overlaid with the anatomical B-mode images in greyscale.   ARG expression in mammalian...production of air-filled protein nanostructures called gas vesicles, which scatter sound waves and thereby...contrast in E. coli. We deleted an unannotated hypothetical protein, improving expression, and named this...of the mARG plamsids into HEK293T cells using a chemical transfection reagent (e.g. PEI-MAX). Simply cotransfect...images of GV expressing MDA-MB-231 cells (top, scalebar = 10µm) and TEM of GVs purified from MDA-MB-231...cell lysate after 4-days of expression (bottom, scalebar = 0.5µm). (d) Ultrasound imaging of MDA-MB-231...

  16. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...said to be representative or the statistical variation is calculated and placed in the legend. Widely ...publication called Scientific Data, which aims to act as a peer-reviewed repository for “scientifically valuable... errors in assumptions, use of inappropriate statistical methods, omission of some data, etc.  The firestorm...months or years before others. In some cases, typically consortia involving genomic screens, the data ...  This means that supporting datasets must be logically annotated and arranged, as in an actual publication...published, this data is usually condensed into a statistically evaluated set of values.  However, to evaluate...likely to improve research. What is published is typically a tiny fraction of what was actually carried out...

  17. Finding Your Science Policy Path

    Type
    Blog Post
    ...backgrounds with technical expertise that ranges from astronomy to bioethics to chemical engineering and...explore their Local Engagement Playbook for step-by-step guides on how to engage in your local community....societal pressures including economic, cultural, and political. Science policy looks differently depending on... p” policy.  “Big P” policy is what you might typically think of. It occurs in a government setting: drafting...in Congress, or can also happen at the state and local level. The big secret about science policy that ...STPF cohort members came to DC to do either a sabbatical year or transition away from academia as tenured...supplement to your career through engagement with your local or state governments. Most communities have opportunities...

  18. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ... the so-called “reproducibility crisis.” Results in preclinical, biomedical and psychological sciences...researchers.There is both theoretical and empirical evidence (in psychology, cancer biology, pre-clinical life science...sciences were called into question after credible attempts to replicate major findings could not be replicated...practices (QRPs) can quickly lead us to find statistically significant results that are not robust. Sampling...research plans become public eventually). It is typically done right before data collection starts, and ...each hypothesis will be tested with a specific statistical test.  Preregistration makes clear the distinction...differences between groups. Hypothesis generation is critical for advancement, but we are too often encouraged...

  19. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...principles to biological systems. Today, what started in Cambridge with a handful of local high school ...BioBuilderClub teams’ biological engineering designs and ideas ranged from a biological breathalyzer to pesticide...School Apprenticeship, preparing local (Massachusetts), economically disadvantaged students for paid summer...into today’s classrooms,” BioBuilder answers the call by offering a variety of educational programs all... Dr. Natalie Kuldell from MIT’s Department of Biological Engineering. Built primarily from her 20 years...experiential learning. “Students may forget the technical details they hear in their classes,” she said,...learning outcomes by emphasizing the importance of critical thinking, persistence, teamwork, and communication...

  20. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...their visions for a successful and sustainable biomedical research sector at our first Future of Research...discussed, many related to the structure of the biomedical workforce. Quotes taken directly from participants...around the USA undertaking BEST programs, including local schools in Boston: the Boston University School ...of Medicine and the University of Massachusetts Medical School. Graduate education programs are also beginning...internship to be taken in an industry, regulatory, or clinical environment. It remains contested, however, whether...shape and inform the overall structure of the biomedical research sector, and what a tenable workforce...Shapes Science’ and contributing to the NIH’s Biomedical Research Workforce Working Group. Along with ...
Showing: 181 - 200 of 1010 results