We narrowed to 469 results for: Lif
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TypeBlog Post... Sponsorship from Addgene and Harvard Integrated Life Sciences (HILS) made it possible to offer DayCon...attendees and getting the chance to hear more about what life is like as a student researcher. The future of DayCon...
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A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog Post... of mutations they identified included gene amplifications and fusions, missense mutations, and indels...cell lines and any mutations that that confer proliferation advantages will be revealed in the absence of... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog Post...employees created and designed for the Hall of Human Life at the MOS. (If you want a fun refresher on the ...of requesting and sharing plasmids has become simplified through Addgene. Looking back, Addgene’s journey... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog Post...recombination is the process by which nearly all domains of life repair genomic damage, specifically double strand...tags have been integrated. Simply design your amplification primers with the desired targeting homology—... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog Post...Children's Hospital California Institute of Technology Johns Hopkins...Sciences University of Texas at Austin University of California, San Francisco University of Pennsylvania Stanford... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...understanding how nuclear organization varies across the life of a cell, or how organization may be altered in...Addgene. A protein-tagging system for signal amplification in gene expression and fluorescence imaging.... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...from the standard UAS/GAL4 system is toxic to proliferating cells. A Cre-estrogen receptor ligand binding...PubMed. Reduction of Cre recombinase toxicity in proliferating Drosophila cells by estrogen-dependent activity... -
London Calling - Addgene’s New Europe Office in London
TypeBlog Post...the University of London and the University of California, specialising in Molecular Biology. She has worked...Genetics. Since then she has worked in the the UK, California, Africa, and Australia, viewing science as a ... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog Post...have increased thermostability also had a longer lifetime in human blood. To test if this was also true ...Lucas, et al. "A thermostable Cas9 with increased lifetime in human plasma." bioRxiv (2017): 138867. Additional... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog Post...coding sequence space. eLife. (2022) 11:e67667. DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P.... -
Plasmids 101: What is a plasmid?
TypeBlog Post...within a cell make them attractive tools for the life scientist or bioengineer. Figure 1: Map...sequence used as an initiation point for PCR amplification or sequencing. Primers can be exploited for ... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog Post... people are paying attention to living a “green” life and creating less waste. Most communities seem ...wasteful packaging. Turns out, one could make a lifetime supply for about $10 US dollars worth of glass... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog Post...tenured faculty position for the grind of corporate life”. In fact, there are a lot of good reasons to ...more structured time management conducive to work/life scheduling Diverse opportunities to do science related... -
Plasmids 101: TOPO Cloning
TypeBlog Post...insert comes from using Taq polymerase for the amplification step since Taq polymerase leaves a single deoxyadenosine... add unique restriction sites on the ends) and amplify your sequence of interest with Taq polymerase using... -
RUBY-Red Siliques
TypeBlog Post... events and am currently planning to use it to simplify my work with an Arabidopsis mutant that can’t ...unfamiliar with these mutant plants. Using RUBY to simplify the identification of these mutants will speed... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog Post...that contain repeating elements. Knowing how to amplify recombination-prone plasmids and testing your prep...without affecting mRNA translation efficiency or half-life. RNA 25:507–518. https://doi.org/10.1261/rna.069286.118... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog Post...partnered with Dr. James Trimmer of the University of California Davis School of Medicine on this grant. Dr. Trimmer...be working with Addgene to bring this resource to life,” says Dr. Trimmer. But in order to create such ... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...at 22. Start with your network You've spent your life making connections and finding mentors -- well, ... evolve and respond to feedback throughout its lifetime. In this way, you will always improve and give... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...repair Adapter Ligation Clean up/Size selection Amplification Clean up I found this kit easy to use because...DNA we started the process with, we got great amplification of our sample libraries. NEB also gave us a ... -
Science Career Options
TypeBlog Post...overtime and weekends, part time or full time) and what life is like for someone who currently in the position...online job websites to see what requirements or qualifications are needed for the role, as this will give ...