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  3. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...Assembly: a) Graphic depiction of chromatin within a nucleus -> DNA compaction around histones -> Examples of...libraries encode target sites flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM...

  4. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...administration. However, until recently safe delivery of nucleic acid cargo (DNA or RNA) to target cells has been...Self-complementary AAV mediates gene targeting and enhances endonuclease delivery for double-strand break repair." Gene...

  5. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...protected by histone-like proteins forming the nucleocapsid. B) Three generations of adenoviral vectors:...purified plasmid stock should be analyzed by endonuclease restriction digest or fully sequenced to confirm...

  7. Hot Plasmids February 2024

    Type
    Blog Post
    ...primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely due to its long,...Cell-penetrating Cas9, fused to HIV TAT, Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess...

  9. Hot Plasmids - October 2020

    Type
    Blog Post
    ... than the more efficient single-stranded oligodeoxynucleotide donors (ssODN). So how can you use CRISPR...codon-optimized, engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-3′ PAM. Get the plasmids...

  11. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ... "WDDD: worm developmental dynamics database." Nucleic acids research 41.D1 (2012): D732-D737. PubMed ... a platform for 3′ UTR biology in C. elegans." Nucleic acids research 36.suppl_1 (2007): D57-D62. PubMed...

  12. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...including mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs ...power of this system lies in the ability of the endonuclease Cas9 to create a DNA double stranded break (...

  15. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...endA+. endA encodes a thermostable periplasmic endonuclease that can sometimes co-purify with your plasmid...recommend performing an extra wash step to remove endonucleases (see figure at right for an example). Look out...

  17. An Introduction to Adenovirus

    Type
    Blog Post
    ...innate immune system; and reconfiguring of the nuclear compartment, gene expression, and protein translation...Adenovirus Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https...

  18. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ..., W. S., Pitkanen, A., & Rubin, H. (1965). The nucleic acid of the Bryan strain of Rous sarcoma virus:...purification of the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences...

  20. CRISPR Antimicrobials

    Type
    Blog Post
    ...antimicrobials using efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed... Bikard, David, et al. “Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials.” ...
Showing: 181 - 200 of 422 results