We narrowed to 658 results for: CHI;

...Matthew J., et al. "Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated...enables simplified chromosomal gene replacement in Escherichia coli." Appl. Environ. Microbiol. 81.15 (2015)...Recombineering) system for genome editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed...

...getting dark and wintry with the end of year approaching. We’re always looking for something to brighten...bright fluorescent proteins with different thermal quenching rates (mNeonGreen and tdTomato), they produce ...demonstrates that taking advantage of fluorescence quenching properties is a promising strategy for developing...

...highlight the specific modifications to CRISPR machinery that allow for the use of CRISPR in plants, and...in question. In plant cells, gRNA expression is achieved by placing the gRNA downstream of a plant-specific...Chloroplast-selective gene delivery and expression in planta using chitosan-complexed single-walled carbon nanotube carriers...

...sequence of the mutated gene the researcher is searching for), then the probe will bind to the sample thereby...generally considered first generation DNA sequencing machines (9). Today, similar technologies, sometimes referred...older technologies nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing...

...fragments. As a fun way to start, I highly recommend watching this entertaining video created by our friends...homology regions, which allows for the mixing and matching of parts. Gibson assembly meets CRISPR Gibson... 1. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO. Enzymatic assembly of DNA molecules...

...family of GFP proteins in the cephalochordate Branchiostoma floridae, named bfloGFPs. They went on to characterize...Gateway cloning methods developed by the lab of Joachim Wittbrodt might be just the set of tools that you...-voltage (LOV) sensing domains from algae into chimeric Opto-RTKs. The LOV domains bind flavin cofactors...purifying Cas9? Check out pET-28b-Cas9-His from Alex Schier's lab, designed for expression and purification ...

...trafficking and their detection can suffer from photobleaching and cellular autofluorescence (Tsien, 1998)...plates or media, which can obscure weak signals; photobleaching from the excitation light, causing the signal...EVs. Find ADAR-HiBiT Fusion Vector at Addgene! Watching protein degradation in real time Proteins involved...

...enhancers. Although the lab used this vector in chick embryos, it also functions in mammalian expression...Both of these methods also recruit epigenetic machinery - but is there an advantage to using direct epigenetic...probing in vivo cis-regulatory interactions in the chicken embryo. BioRxiv: https://doi.org/10.1101/135525...

...transfections and are therefore virus-free. This is achieved using the piggyBac™ transposase, which facilitates...using the piggyBac™ transposase. This can be best achieved by utilizing a piggyBac™ transposon in a donor...the piggyBac™ donor is used by the cell’s host machinery for DNA repair by homologous recombination, which...

...related LCR technique uses two sets of probes to achieve exponential amplification. What does fidelity mean...substrates. Thus, high fidelity ligation can be achieved through a combination of the intrinsic selectivity...Thermus thermophilus DNA ligase, a homolog of Escherichia coli DNA ligase." Journal of bacteriology 173.16...

...to stars in space ships and the rise of killer machines that exterminate entire humanity were the two ... technologies rely on the cellular DNA repair machinery, which can lead to high frequency of indel formation... the use of molecular dynamics simulations and machine learning to develop a predictive understanding ...

...membrane hyperpolarization but unlike HR, this is achieved through hydrogen ion efflux; evidence suggests...expression of your opsin is no longer required to achieve cell-type specific opsin expression. However, the...Cellular and Molecular Biology from the University of Michigan and he is currently a Postdoctoral Fellow in the...

.... DNA quantity is fairly self explanatory and achieved by performing different sized preps, such as miniprep...of your plasmid prep is by spectrophotometry. Achieving the highest-quality DNA requires taking care at... are designed to work together — no mixing and matching!   Figure 2: Plasmid isolation workflows...

...pluripotency of mouse cells typically involves making chimeras by injecting iPSCs into blastocysts and implanting...implanting them into female mice. Generating chimeric offspring shows the ability of the iPSC to contribute... three germ layers, and the ability of theses chimaeras to produce all-iPSC derived offspring demonstrates...

...strength, and binding (e.g. DHS-seq, FAIRE-seq, and CHIP-seq), genome-wide identification and quantification...to test multiple expression systems before they achieve the appropriate expression level for their gene...cloning process alone involves much literature searching, DNA synthesis, and plasmid assembly. Luckily,...genome-engineering endeavours of unparalleled complexity in Escherichia coli, like the construction of a so-called “genomically...

...Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki...sites previously difficult to target. ABE8s can achieve 98-99% target modification in primary T cells, ...

...incorporates a bioluminescence protein into the chitin that can be activated only by a specific frequency...Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References Abril, U.N.O., et....

...Plant Breeding, Genetics, and Biotechnology at Michigan State University with the backing of a prestigious...:47-9:35 : Kingdom's biotechnology research at Michigan State University 9:36-10:29 : Kingdom's transition...

...organizing Halloween festivities for years – searching our photo archives I found pictures back through 2009, ...

...AAV9 Biosensors Patriarchi New viral service pAAV_hSyn1_nLightR AAV9 Biosensors Patriarchi New viral service...

... your own depositor metrics!  About the Author Joachim Goedhart is an assistant professor at the Section...fluorescent probes. You can follow him on twitter: @joachimgoedhart.    ...

...MerryChristmas #happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan @AnoeskaMoosdijk...Fireplace from the Dyer Lab If you’re feeling as chilly this winter as the Addgenies are in Massachusetts...

...ending the day with 1,000,002 plasmids shipped. Reaching one million plasmids shared might have you wondering...colossal 547 years! While we are very excited about reaching this milestone, it wouldn’t have been possible...

...was a fantastic month for the Addgene blog. We achieved roughly 50,000 views over the entire blog and ... Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo As always...

...lab equipment to carefully-screened research and teaching departments at universities around the world. ...the Addgene Blog Learn About Our Recent Visit to China Read Zafira Castano Corsino’s Recent Post on the...

... DSHB was created by the National Institute for Child Health and Human Development (NICHD) in 1986, to...toxic adjuvants; 2) generating complex antibody chips that allow assessment of many protein targets in...

...establish.These funding decisions will have far-reaching consequences throughout the scientific community... can turn to Addgene as a trusted partner for archiving their biological materials during funding transitions...

... Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're thinking about hosting multiple plasmids...incompatible. 4. RNA Extraction Without A Kit by Leah Schiesow We added to our protocol collection this year...

...Making the Right Connection by Joachim Goedhart Guest blogger Joachim Goedhart shares some strategies...

...contamination, but consider flow cytometers. These fluidic machines handle biological samples and are cleaned between...between each use. You wouldn’t throw away your FACS machine after a single use. So how about washing and reusing...