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We narrowed to 650 results for: CHI;

Showing: 271 - 300 of 650 results

  1. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... lab. Some are rabbit immunoglobulin G1 (IgG1) chimeric antibodies, and others are rat or mouse IgG2a....reproducible rat or mouse rAbs. The other eight are chimeric antibodies composed of rat or mouse variable regions...antibodies comes from IPI’s work with the Boston Children’s Hospital and Harvard-based lab of Tim Springer...

  2. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...getting dark and wintry with the end of year approaching. We’re always looking for something to brighten...bright fluorescent proteins with different thermal quenching rates (mNeonGreen and tdTomato), they produce ...demonstrates that taking advantage of fluorescence quenching properties is a promising strategy for developing...

  3. Evolution of Lab Techniques

    Type
    Blog Post
    ...sequence of the mutated gene the researcher is searching for), then the probe will bind to the sample thereby...generally considered first generation DNA sequencing machines (9). Today, similar technologies, sometimes referred...older technologies nanopore-based DNA sequencing machines are relatively small meaning that DNA sequencing...

  4. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...fragments. As a fun way to start, I highly recommend watching this entertaining video created by our friends...homology regions, which allows for the mixing and matching of parts. Gibson assembly meets CRISPR Gibson... 1. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO. Enzymatic assembly of DNA molecules...

  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...family of GFP proteins in the cephalochordate Branchiostoma floridae, named bfloGFPs. They went on to characterize...Gateway cloning methods developed by the lab of Joachim Wittbrodt might be just the set of tools that you...-voltage (LOV) sensing domains from algae into chimeric Opto-RTKs. The LOV domains bind flavin cofactors...purifying Cas9? Check out pET-28b-Cas9-His from Alex Schier's lab, designed for expression and purification ...

  6. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...enhancers. Although the lab used this vector in chick embryos, it also functions in mammalian expression...Both of these methods also recruit epigenetic machinery - but is there an advantage to using direct epigenetic...probing in vivo cis-regulatory interactions in the chicken embryo. BioRxiv: https://doi.org/10.1101/135525...

  7. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...transfections and are therefore virus-free. This is achieved using the piggyBac™ transposase, which facilitates...using the piggyBac™ transposase. This can be best achieved by utilizing a piggyBac™ transposon in a donor...the piggyBac™ donor is used by the cell’s host machinery for DNA repair by homologous recombination, which...

  8. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...to stars in space ships and the rise of killer machines that exterminate entire humanity were the two ... technologies rely on the cellular DNA repair machinery, which can lead to high frequency of indel formation... the use of molecular dynamics simulations and machine learning to develop a predictive understanding ...

  9. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...related LCR technique uses two sets of probes to achieve exponential amplification. What does fidelity mean...substrates. Thus, high fidelity ligation can be achieved through a combination of the intrinsic selectivity...Thermus thermophilus DNA ligase, a homolog of Escherichia coli DNA ligase." Journal of bacteriology 173.16...

  10. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...membrane hyperpolarization but unlike HR, this is achieved through hydrogen ion efflux; evidence suggests...expression of your opsin is no longer required to achieve cell-type specific opsin expression. However, the...Cellular and Molecular Biology from the University of Michigan and he is currently a Postdoctoral Fellow in the...

  11. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    .... DNA quantity is fairly self explanatory and achieved by performing different sized preps, such as miniprep...of your plasmid prep is by spectrophotometry. Achieving the highest-quality DNA requires taking care at... are designed to work together — no mixing and matching!   Figure 2: Plasmid isolation workflows...

  12. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...pluripotency of mouse cells typically involves making chimeras by injecting iPSCs into blastocysts and implanting...implanting them into female mice. Generating chimeric offspring shows the ability of the iPSC to contribute... three germ layers, and the ability of theses chimaeras to produce all-iPSC derived offspring demonstrates...

  13. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...strength, and binding (e.g. DHS-seq, FAIRE-seq, and CHIP-seq), genome-wide identification and quantification...to test multiple expression systems before they achieve the appropriate expression level for their gene...cloning process alone involves much literature searching, DNA synthesis, and plasmid assembly. Luckily,...genome-engineering endeavours of unparalleled complexity in Escherichia coli, like the construction of a so-called “genomically...

  14. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki...sites previously difficult to target. ABE8s can achieve 98-99% target modification in primary T cells, ...

  15. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...incorporates a bioluminescence protein into the chitin that can be activated only by a specific frequency...Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References Abril, U.N.O., et....

  16. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    ...Plant Breeding, Genetics, and Biotechnology at Michigan State University with the backing of a prestigious...:47-9:35 : Kingdom's biotechnology research at Michigan State University 9:36-10:29 : Kingdom's transition...

  17. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...organizing Halloween festivities for years – searching our photo archives I found pictures back through 2009, ...

  18. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...MerryChristmas #happyholidays2018 #DeckTheLab @Addgene @joachimgoedhart @SAbiega @EikeMahlandt @Saskia_deMan @AnoeskaMoosdijk...Fireplace from the Dyer Lab If you’re feeling as chilly this winter as the Addgenies are in Massachusetts...

  19. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...AAV9 Biosensors Patriarchi New viral service pAAV_hSyn1_nLightR AAV9 Biosensors Patriarchi New viral service...

  20. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    ...ending the day with 1,000,002 plasmids shipped. Reaching one million plasmids shared might have you wondering...colossal 547 years! While we are very excited about reaching this milestone, it wouldn’t have been possible...

  21. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ... your own depositor metrics!  About the Author Joachim Goedhart is an assistant professor at the Section...fluorescent probes. You can follow him on twitter: @joachimgoedhart.    ...

  22. Editor's Choice, July 2016

    Type
    Blog Post
    ...was a fantastic month for the Addgene blog. We achieved roughly 50,000 views over the entire blog and ... Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo As always...

  25. Addgene Stands with the Scientific Community

    Type
    Blog Post
    ...establish.These funding decisions will have far-reaching consequences throughout the scientific community... can turn to Addgene as a trusted partner for archiving their biological materials during funding transitions...

  26. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ... Plasmids 101: Plasmid Incompatibility by Leah Schiesow If you're thinking about hosting multiple plasmids...incompatible. 4. RNA Extraction Without A Kit by Leah Schiesow We added to our protocol collection this year...

  27. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...contamination, but consider flow cytometers. These fluidic machines handle biological samples and are cleaned between...between each use. You wouldn’t throw away your FACS machine after a single use. So how about washing and reusing...

  29. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes plasmids designed for...specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory capacities of ...
Showing: 271 - 300 of 650 results