We narrowed to 877 results for: HA

...repository, and getting feedback from our community. It has been great to hear all the words of support from ...from scientists using Addgene in their labs. And it has been exciting to learn about all the amazing research...

...of just how big and mainstream the news on CRISPR has gotten, consider these headlines in The New York ...New York Times feature when he said, “All of this has basically happened in a year. It’s incredible.” ...

...Adenovirus is a linear, dsDNA virus naturally. It has ITRs, just like AAV, which encompass the expression...packaging and entry Adenovirus is quite large and has more helper plasmid components than the other viruses...of adenovirus Building viral vectors Addgene has many viral vectors and helper plasmids for all the...

...radioactive tracer. As a solution, Mikhail Shapiro’s lab has developed four acoustic reporter gene (ARG) plasmids...connection between specific genes and an immune response has been a longstanding challenge. Using shRNA, TALEN... for in vivo functional analysis of immune cells has been a gap in the field. Arlene Sharpe’s group at...

...in vivo scientists need a protease reporter that has a large dynamic range and high brightness. The Shu...in the midgut of Drosophila, an observation that has been difficult until now (Zhang et al., 2019). Conclusion...Conclusion FlipGFP is a novel fluorogenic reporter that has a large dynamic range and high brightness. This reporter...

...impressed with is the level of proficiency that Addgene has developed in terms of the collection, storage, and... What is the best piece of career advice someone has given you? Katz: Pick up the phone. I think that ... insurance, finance and high-tech industries. He has earned both an MBA in finance and a B.S. in accounting...

...15 years of plasmid sharing has certainly taken us on many adventures...From moving office locations ...plasmids are from the Zhang lab. In fact, the Zhang lab has shared over 40,000 CRISPR-Cas9 components over the...Chimeric_BB-CBh-hSpCas9 - The S. pyogenes Cas9 (SpCas9) has been widely popular in genome engineering. For use...

...restricted based on experimental constraints. This has been used for a wide range of purposes, including...Cre-containing adenovirus (Ad-Cre) or AAV (AAV-pgk-Cre) has been used to successfully introduce Cre into cells... can be an effective alternative to Cre-lox, and has also been used in conjunction with it, allowing for...

...in bacteria, most CRISPR-based genome engineering has taken place in other organisms. In many bacteria,... targets cells in which homologous recombination has not occurred. This powerful negative selection system... works similar to the original pCas/pTargetF but has many other advantages including shorter plasmid curing...plasmids Noting that the more common spCas9 system has generally low efficiencies or can be proteotoxic ...system can be delivered a number of ways but success has been observed with Phagemids (Selle et al., 2020,...microbiome Beyond the human gut, the Barrick lab has deposited plasmids that can be used to engineer the...

...Henry Bourne, Professor Emeritus at UCSF. Dr. Bourne has some radical (and some not so radical) suggestions...called The Balsa Group. University of Pennsylvania has the Penn Biotech Group and PBG Healthcare Consulting...

...and you need the right experimental materials. Who has time for paperwork? Here’s why a Material Transfer...confidence. Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform Biological Material...

...effective than both AAV9 and AAV5, which has tropism for lung cells, and has increased overall transduction throughout...this definition: A systemic capsid is a capsid that has been engineered to have enhanced function after intravenous...both). Therefore, a high-efficiency systemic capsid has better efficacy than AAV9 and/or its intermediate...used in LY6A-nonpermissive mouse strains. **AAV1-X1 has not been tested in rats. For more information on ...neurons in the CNS (Chan et al., 2017). AAV-PHP.eB has frequently been used for screening candidate regulatory...depositing manuscript) across the brain. AAV-BI-28 has been tested in C57BL/6J mice and effectively transduces.... 4c, Jang et al., 2023; Brown et al., 2021.) It has a similar transduction rate to AAV-PHP.eB in the ...

... to its unrivaled contrast, fluorescence imaging has emerged as the dominant light microscopy contrast...blue to excite a molecule of GFP. Because red light has approximately half the energy of blue light, two ... be establish for this to happen.Using red light has a second advantage: red light penetrates deeper into...imaging depths closer to 1 mm. Traditionally, 2-photon has been a very slow imaging technique but recent advances... modern explosion in light microscopy techniques has provided biologists with a host of tools and opened...

... cell mobility within these complex environments has never been easy and, for many years, researchers ...Intravital imaging powered by two-photon microscopy has uncovered host-pathogen interactions leading to the...proteins can be used in your experiments, experience has shown that some are more reliable and more efficient...important to finely tune the excitation wavelength as it has been shown that even a small incremental change of...this system called Brainbow, the Ubow mouse strain has been created to fate map Langerhans cells and follicular...

... a lot quicker to pick up new languages!) Jargon has a place in science communication, but the more you...length of your average PhD to achieve!) Each field has its own framework: a particular way of organizing... amount of knowledge and expertise your audience has is a major factor in how quickly they can understand...

...antibody is biotin-conjugated, streptavidin (which has a high affinity for binding biotin), is conjugated...detecting one protein from the sample. A direct ELISA has many fewer steps than a sandwich or competitive ELISA...linear portion or the OD of your experimental samples has reached 4.0, you can resolve this issue when you ...

...all four parts of a STAR answer once the interview has started. Instead, I preferred to prepare stories ...stories for an interview, since one story usually has more than one theme and can be used to answer multiple...new idea to the company’s area of expertise. This has potential to make your audience really sit up and...

...VSV-G that generally mediates broad cell tropism and has a high percentage of neuronal targeting after stereotaxic... damage to the brain tissue. The injection speed has to be slow to allow the viral particles to diffuse...order to minimize the pressure into the tissue, but has to be large enough to target larger areas, typically...

...the Fire-Cas9 Podcast Segment Jerry Crabtree’s lab has developed a new CRISPR/Cas9-based system to study.... While catalytically inactive cas9 (e.g. dCas9) has been fused to a variety of protein modules to repress...synthetic transcription factors, the El-Samad lab has deposited plasmids encoding fluorescent reporters...

...called the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent...visualize genomic loci in vivo The flexibility of CRISPR has improved our ability to target almost every genomic... system and induce genome editing. Such a system has been previously described for transcriptional activation...

...CombiGEM-CRISPR: The marriage of two simple platforms CRISPR has revolutionized how we decode the genome by making...guide RNAs (gRNAs) for genome editing in large-scale has led to the rapid generation of genome-wide gRNA libraries...the users’ specific research questions. CombiGEM has been successfully applied to functionally characterize...

...auditory neuronal circuitry in vivo. A variant of RABV has been developed to only label a neuronal connection...example only dopaminergic neurons, neuronal tracing has the added benefit of only labelling the subset of... regions used. Evidence for retrograde transport has been found for AAV1, -2, -5, -7, -8 (Taymans et al...AAV5 (Aschauer et al., 2013). Anterograde transport has been observed for AAV1, -5, -8 (McFarland et al.,...variability compared to chemical tracers. rAAV2-retro also has lower affinity for heparin, which possibly explains...vectors cover some of these attributes and their use has allowed for the visualization of single connection...intracerebral application, which is more invasive and has a much higher risk of complications. References...

...IgG2a  Rat   Targeting integrins in the lab The Ha and Springer labs recently showed RGD-binding integrins... interpret the roles of individual integrins. The Ha lab — in collaboration with the Springer lab, the...Leucine-Valine-Aspartate-Proline (LVDP) motif. The Ha and Springer teams employed IPI synthetic antigen-binding... J., Yan, J., Waterman, C. M., Springer, T. A., & Ha, T. (2022). Single-molecule characterization of subtype-specific...

...in the same field that I do: prostate cancer. She has guided me in my next steps. For example, how to get...to travel abroad, how to write cover letters, and has suggested important things to emphasize in order ...insecurities… all shrink when shared with a mentor who has experienced them before and who can serve as a role...universities and future generations of professionals has made possible a significant qualitative and quantitative...

...lentiviral service is going strong, the AAV service has shown incredible growth this year. pAAV-hSyn-DIO-...pmSyn1-EBFP-Cre from Hongkui Zeng’s lab. This AAV has had over 150 orders since coming online! pmSyn1-EBFP-Cre...

...10 min and then recheck the pH to ensure that it has not drifted. Filter the solution through a 0.22 μm...

...mL) x dilution factor e.g., If the 1:100,000 well has 75 colonies, then there are 75 colonies /1.5 mL x...

...a look at the secondary antibodies that your lab has on hand and choose a primary antibody that will work...secondary antibody pairing such that each target has a unique species or isotype that can be probed with...polyclonal primary antibodies.  I hope this post has explained when and why antibody isotypes matter and...

...the idea that DNA methylation is linked to disease has now become much like the 1980s song “Don’t Stop Believin...none existed. Recently, however, a particular tool has gained widespread popularity – and notoriety – as...may later be excised by Cre recombinase. Addgene has several great options: pLV hUbC-dCas9-T2A-GFP for...

...Cas12a. This is in part due to the fact that it only has a single active site. The RuvC active site in Casɸ...rely on multiple active sites. In addition, Casɸ has a minimal PAM requirement. For instance, for Casɸ...library here A new SaCas9 ABE variant (microABEI744) has improved on-target editing efficiency and reduced...

...version of PubMed.  Key features This new version has an improved search algorithm which was trained on...your search terms. Key features Semantic Scholar has several ways to focus your search results. A bar ...paper’s entry, the number of citations that paper has is displayed at the top of the page, as well as the...

...community has always emphasized the need for open science and collaboration. The field already has comprehensive...first virtual organism in a computer.” This project has produced various resources for the worm community...

... a raise in salary, particularly given that this has precipitated increases to the NIH recommended pay...both of these changes share a common thread that has the potential to profoundly shift the academic science...rather than as trainees in a special category, as has been the ‘labor-of-love’ philosophy of academic science...

...Epigenetics has recently been hitting the headlines, with sotires like the potential devastation of the...them. Global hypomethylation, on the other hand, has also been implicated in the development and progression...difficult to overcome until now. Alice Ting’s lab has developed a new, plasmid-based biosensor to visualise...

...    In the video above, Channelrhodopsin 2 (Chr2) has been expressed in the motor cortex of a mouse. Upon...appropriate wavelength to stimulate your opsin. Each opsin has an excitation spectrum (use the resources discussed...controlled. As discussed above, each opsin variant has slightly different activation/inactivation kinetics...

...your supervisor’s expectations Every supervisor has their own management style. A hands-on supervisor...agree with all of your ideas, but he or she probably has reasons for it. Do not take criticism personally ... of Finish Your Thesis, an online community that has supported thousands of graduate students since 2009...

... Our Editor's Choice Blog Posts The Addgene Blog has had quite the up-tick in views over the month of ...recommend taking a look at all of our posts as each one has something great to offer, I just need to get back...

...developed for use in mammalian cells, this system has also been implemented in a variety of other organisms...Drosophila cells. Work by Wei et al. from Kang Shen’s lab has also shown that the Q system is also functional in...elegans. Subsequent work by the Luo and Potter labs has refined the Q system and introduced the second generation...multiple genes in synthetic systems. Recent work has even used all 3 in a single modular vector for ease...

...top of the resolving gel. A stacking gel typically has a different ionic strength and lower pH and acrylamide...Choosing your primary antibody Once the membrane has been blocked, it is stained with a primary antibody...the vendor’s website and choose an antibody that has been validated for immunoblotting. Different immunoassays..., cytoplasm and nucleus.  We hope that this blog has provided you with a good overview of the western ...

...facilities, tissue culture labs and every cell culturist has to deal with this problem. It is estimated that mycoplasma...this induces a noticeable color change. This method has high sensitivity but low specificity (Degeling, 2012...affect the cell growth and viability. If mycoplasma has made permanent damage to the cell culture it is irreparable...Institute of Biosciences and Technology in India. Jadhav has a Master's degree in Molecular Biology (University...