... the HA frankenbody? Another large advantage of the HA frankenbody is that it remains bound to HA epitopes...feature provides HA frankenbody the ability to bind nascent HA tagged proteins.  Lastly, the HA frankenbody...the widely used HA epitope in diverse cellular environments. They called this probe the HA frankenbody (... towards a HA epitope from the CDRs.  To visualize proteins of interest tagged with the HA epitope, the...spaghetti monster HA (smHA) which contains a 10xHA tag. These experiments demonstrated that the HA frankenbody...localization of single HA-tagged molecules over time.  The Stasevich lab also proved that HA frankenbody can ...proteins.  Limitations with GFP in imaging  While GFP has been an immensely powerful tool to study proteins...

...indicates that a plasmid has been requested 20+ times   A red flame means a plasmid has been requested 50+ ... Castro of University of Michigan says, “Addgene has transformed the way in which we share plasmids and...making it easier, cost effective and faster; this has helped accelerate scientific discoveries.” Occasionally...+ times   A blue flame means a plasmid has been requested 100+ times Since we so sincerely appreciate ...have at least one plasmid deposited at Addgene that has been distributed more than 100 times. We hope these...sharing because, as UNC’s Bryan Roth says, Addgene has “literally enabled sharing with 100's of investigators...popularity of CRISPR in the plant community, this has saved us hours of work responding to requests from...

...discussed in more detail below. The HA tag can be stained for with an anti-HA antibody and serves as a backup...detected either with a labeled anti- HA or -MYC antibody or with an anti-HA or -MYC primary and a labeled secondary...Gelsolin Spaghetti Monster: HA (H) or Myc (M) versions Labeled anti-HA or -Myc antibody Staining cultured...detectable by two different tags: a reporter and an HA tag. The reporter comes in three varieties: HRP, ...independent of the reporter. The only caveat is that HA staining is weaker than reporter staining. Picking...Spaghetti Monsters are highly antigenic tags that have 10 HA or 10 MYC tags built into a GFP scaffold. Some GFP...recognize this GFP scaffold itself. Fortunately the GFP1 HA Spaghetti Monster RANbody doesn’t recognize the scaffold...

...base editor pCMV-BE3 has been requested nearly 400 times, and newcomer pCMV-ABE7.10 has already earned a ... CRISPR has made it possible to design programmable epigenome editing tools, and the field has certainly...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that...have available by category. Each plasmid page now has a helpful graphic to introduce you to the plasmid... the Transcriptional Regulation category already has five blue flame plasmids covering three different...LwCas13a guide expression backbone with U6 promoter has already earned a blue flame. RNA Editing: Did anyone...

...Addgene has you covered with monoclonal antibodies to Myc for all of these species. HA The HA tag is ... The tag has two negatively charged amino acids for an overall negative charge. This tag has been used...charged and 2 positively charged amino acids. FLAG tag has been used with moderate to high success rates across...from the protooncogene of the same name. The tag has a net negative charge with 4 negatively charged and...antibody can’t be used or that step can be skipped. It has the flexibility of also being used for immunoprecipitation...antibodies or guidance on designing a GFP tag Addgene has you covered!   Potential tag issues Tags have a lot...

...misdiagnosed, meaning that by the time it has caught it has usually started to spread (metastasize) and...and treatments such as these has brought a lot of promise to a field that has historically been full of ...have spread while Stage 4 indicates that the cancer has spread from where it originated to another organ ...However, as the U.S. Environmental Protection Agency has noted, there is no safe level of asbestos exposure...abdominal cavity. This targeted chemotherapy approach has been shown to be successful in killing many (if not...patients: early detection and immunotherapy.  It has long been known that one of the best ways to improve...standard treatments and early detection, mesothelioma has proven itself to be susceptible to up and coming ...

... an essential aspect of biological research that has defined our understanding of biological processes...model organisms is Escherichia coli. The bacterium has been used as a model organism for over 60 years with...cloning and metabolic engineering.  The E. coli genome has an extensive research history and characterization...studies. For example the E. coli biotin ligase, BirA, has been used to identify and study protein-protein interactions...APEX (Rhee et al., 2013). Research using E. coli has the potential to address many areas of microbial ...research with an archive of information and data that has yet to be produced with mice (Iannaccone et al., ..., 2009). While the rat as a model organism has its perks, it is far from perfect. One of the most limiting...

...Doench has been working on developing CRISPR pooled libraries for use in genetic screens and has contributed...Perturbation Platform at the Broad Institute and has worked with many Addgenies to help improve the understanding... time here, this is an incredible place.” Doench has spent most of his research career working on miRNAs...Platforms with a capital ‘P’ focused on technology ... has been critical to the Broad’s success over the last...with David Root in the then RNAi Platform, which has since evolved into the Genetic Perturbation Platform...of CRISPR, the hype was completely justified… it has been just an incredibly useful technology.” He goes...known of Addgene basically since the beginning and [has] been using Addgene ever since” and that he’s “very...

...the target. CRISPR has revolutionized the generation of knockout cell lines which has made this control...In this blog we will review how CRISPR technology has brought drug and genetic screens together and expedited...candidate genes! Disease model generation CRISPR has made the generation of genome edits accessible, fast... have the precise disease genetics. Since CRISPR has been adapted for use in primary cells, many tissue...drug is equally toxic to both lines, it probably has either high toxicity or high off target effects, ...  CRISPR and drugs in the future While CRISPR has already done a lot for drug discovery, there is more...engineered to have many unique capabilities which has advanced what type of edits can be made in the contexts...

...the software. The Inkscape website has many tutorials and Lynda has a tutorial as well, which you may ...to draw figures. It’s part of Google Drive so it has a similar interface as Google Docs or other products...create graphics for the Addgene blog. However, it has a limited number of drawing tools, so it’s harder...web and a desktop version of this software. Vectr has layers, which let you lock and hide individual vectors..., Mac OS X, and Linux. Inkscape is versatile and has a wide selection of tools for drawing and editing... team & I have poured our hearts into for 2 yrs) has 200K+ figures by 95K+ scientists 👨🏽‍🔬👩‍🔬! We...May 22, 2019 Smart Servier Medical Art This site has 3,000 free medical images organized into four main...

...Michael Davidson’s lab at Florida State University has been the go-to place. In 2012, his National High ...backbones alone,” said Addgene’s Lianna Swanson, who has been working with members of Davidson's lab to coordinate...coordinate the impressively big deposit. “He has every fluorescent protein under the sun, from the standard... easy proposition, explained Michelle Baird, who has created plasmids as a technician in the Davidson ...Connection.  mEmerald plasmids and many more! Baird has a helpful tip for anyone looking to peruse the collection...from the nucleus to cell membrane, this collection has fluorescently tagged versions of many proteins known...his life’s work and the stage IV lung cancer that has now prompted him to deposit the tools he and his ...

...page. You’ve called. You’ve emailed. And Addgene has been there to answer your plasmid questions. But ...Addgene’s request statistics Since 2004, Addgene has shipped more than 75,000 plasmids to more than 1,...alone, the number of plasmids distributed to Europe has doubled going from 9,500 in 2010 to more than 20,...London, Emma Markham. Emma was born in England but has since studied and worked around the world, carrying...California, specialising in Molecular Biology. She has worked in a range of labs - cancer research, microbiology...Imperial College London in Genetics. Since then she has worked in the the UK, California, Africa, and Australia... exchange research materials in an easy way. She has a passion for science, loving nothing more than discussing...

...As we mentioned a few months ago, Addgene has been working with Seeding Labs to provide plasmid grants...their research. Louis Bengyella Dr. Bengyella’s work has focused on elucidating the molecular mechanisms behind...potatoes and their microbial pathogens. This work has given Dr. Bengwella expertise in using bioinformatic...Kwabena O. Duedu Dr. Duedu’s molecular biology work has focused on using various cloning methods and tools...Future awardees Now that this first round of awards has successfully concluded, Addgene and Seeding Labs ...

...This competition has gained so much public interest for its stunning images that is has been featured in...Over the past decade there has been a shift in how science is perceived - where once science was only...Queensland, whose outstanding marine photography has attracted much attention over the past few years,... went on to discuss his work: “Macro photography has been my passion for a while - a tool to expand perception...non-scientist alike. “The other thing photography has shown to me - it requires an awful amount of skill...

...Professor of Chemistry at Connecticut College who has been teaching and communicating science for over ... steadily increasing distrust the general public has in science and scientists (as discussed in Chapter...biology also falls within this section. The GFP story has its own Franklin in Doug Prasher, who originally ...advances, and posits the way science is being done has changed significantly over the last decade. He describes... distribution of the newest CRISPR-Cas plasmids, has been a key driver of the technology.” As an Addgene..., I’m very familiar with the role our repository has played in these fields, and seeing names like Feng...have not changed in “COVID times,” but the pandemic has certainly exacerbated them and forced scientists ...

...Repair has been viewed 219,289 times, while Validating Your Genome Edit has 76,011 views.  Has all of ...libraries Addgene has distributed (1C), from 2012 to 2023. Addgene’s collection of CRISPR tools has grown steadily...2023, our CRISPR guide has been viewed 1,904,462 times, and the CRISPR 101 eBook has been downloaded over...globe. Our first story focuses on a technology that has changed virtually all of biology…CRISPR.  Since ...own to share. Over the years, Addgene’s repository has grown to include many CRISPR tools.  Distributing...

...enzymes on the block? The Cas family of proteins has many useful genome engineering enzymes, each with...to generate a blunt DNA double strand break. Cas9 has been studied for genome engineering purposes far ...far more than any other Cas family protein and has thus benefitted from countless rounds of optimization...effective at wiping out large regions of DNA and has anti-viral application potential as a result. Fast... Cas enzymes outlined below as well. Cas12a also has significant therapeutic promise due to the fact that...to package for therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently...for efficient, multiplexable RNA knockdown. Cas13 has also stirred up interest for its potential as a viral...

...some of the great biological societies that Addgene has had the opportunity to work with at Freshers Fairs...Society, England Since 1996, Cambridge Biosoc’s goal has been to promote interest in the biological sciences...students with their applications. The society currently has 1,283 members, who comprise undergraduates, graduate...who want to study chemistry. The society currently has 352 members, ranging from undergrad all the way up...Society based at the University of Oxford currently has around 700 members and hosts a range of scientific...College London for students in this emerging field. It has gained over 170 members in its first year. The society...

...immunoglobulin intron that has been commonly used in constitutive splicing studies and has been optimized for ...light-activated protein domains to the appropriate locus has been cumbersome. To overcome many of the limitations...effectors or Zinc Finger Nucleases, the Gersbach lab has modified the RNA-guided CRISPR-Cas9 system to create...co-localize the VP64 transactivator with a dCas9 that has been targeted to a very specific site on the genome...intracellular pH regulation is important as pH regulation has roles in many cellular functions, from endosomal ..., this peptide is caged by the asLOV2 domain and has reduced affinity for its binding target, SspB. In...activating endogenous genes Moritoshi Sato's Lab has engineered CRISPR-Cas9 to create a photoactivatable...

...protein–protein interactions. The Roux lab has deposited HA tagged BioID2 for N-terminal fusions and Myc... activation of these receptors. Bryan Roth's lab has a newly available PRESTO-TANGO kit which allows researchers...the NCI RAS Initiative, led by Dominic Esposito, has recently released a collection of 360 Ras pathway...24395366 Oxford Drosophila CRISPR library Addgene has had the privilege of distributing several human and...this 3-day goal is is now attainable. The Jacks lab has recently introduced a new assembly cloning platform...Addgene to help expand the collection! The Jacks lab has made several GMAP-compatible plasmids available to...laboratory of Jason Moffat at the University of Toronto has created the Toronto KnockOut (TKO) CRISPR Library...