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We narrowed to 1,006 results for: RING

Showing: 361 - 400 of 1006 results

  1. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ... what challenges remain in materials sharing, information sharing, and research reproducibility while ...service and support.  Alongside our material offerings, in 2022 we also rolled out an updated AAV Data...around the world can upload AAV validation data, sharing what works and, equally importantly, what doesn...commitment to supporting the research community through sharing science and distributing high-quality materials...

  2. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...relatively simple in C. elegans and bacteria. But CRISPRing typical mouse models, including creating Cre-dependent...Megamer service tested by Quadros et al. When delivering the ssDNA template and CRISPR machinery, Quadros...facilities for 13 projects, with most projects requiring only 40-60 zygotes to generate a correctly edited...conditional alleles by CRISPR/Cas-mediated genome engineering." Cell 154 (2013):1370-9. DOI: 10.1016/j.cell... a robust method for in situ germline genome engineering using CRISPR nucleases. Genome Biol. 19 (2018... Mary Gearing...

  3. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...Westerns are divided into two categories, native and denaturing. In a native western, the protein’s secondary...is separated through a matrix by charge. In a denaturing western, the protein is denatured to its primary... purpose of this blog, we will be focusing on denaturing westerns.  Separating a protein mix by size Sodium...The wet transfer process is very effective in transferring a broad range of protein sizes but takes hours...blocking the membrane  from binding to the antibodies during the stain. The ideal blocking buffer depends on...loading control when assessing protein abundance or comparing samples across lanes.  For a typical loading control...

  4. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    ...social media, you could be sharing your research or finding opportunities to bring science to the community...the same page with the potential PI in terms of mentoring style and expectations. Make sure you will enjoy...mantra and never forgot it. Usually when I see inspiring artists who are thinking about getting into the... the process down into steps and set aside time during the week to tackle each one.  Get the toughest ...

  5. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    ...year, graduate students and post-docs alike are wondering where the time has gone. Are we any closer to ...to graduating, publishing that key paper, or figuring out a career path? Many trainees are developing Individual...accomplishments, from learning new techniques to finally figuring out a tough experiment, sets a positive and encouraging...Good Manager Anyway Developing Transferable Skills During Science Training ... Mary Gearing...

  6. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    .... pombe. The protocol is surprisingly simple, requiring only a PCR product containing the modifying sequence...affinity purification of protein complexes. John Pringle and Jürg Bähler have deposited a large collection...genome modification in yeast developed by Dr. Pringle’s former lab at UNC Chapel Hill. Bähler et al.(2...McKenzie A 3rd, Steever AB, Wach A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):943-51. Longtine MS,... DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):953-61. Note: Marcy...

  7. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...believing, as we do, that sharing speeds science.  This year, Chonnettia will be sharing more of her perspective...what we accomplished in 2023 and thrilled to be sharing our plans for 2024.  In 2023, Addgene distributed... of innovation and impact made possible by the sharing of tools for research: discovered and developed...you a healthy, productive, and thriving 2024, mirroring our own aspirations for Addgene. On behalf of ...

  8. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    ...openness and sharing in science.  We’re excited to support the neuroscience community by sharing not only ...capsid proteins that determine the AAV serotype. During transduction, the viral capsid proteins bind to...enhancing anterograde transport efficiency, and engineering viral capsids that target specific cell types...science, we aim to accelerate the rate by which sharing speeds science.   Check out the precise neuronal...

  9. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...new technique, developed in Thoru Pederson’s lab, brings us one step closer to mapping the 4D nucleome, ...with the previously established physical map. In comparing pairs of targets ~2 Mbp apart, they noticed that...may be imaged under lower illumination settings, lowering photobleaching and phototoxicity concerns and ... of chromosomal translocations or chromosomal shattering (chromothripsis). As this study joins the pantheon... Mary Gearing...

  10. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...DCL) process the dsRNA into specific types of interfering RNAs.   RNAi vs. CRISPR: Complementary techniques...fundamental steps: creation of the RNAi trigger delivering the RNAi trigger characterizing the RNAi phenotype...from a long hpRNA trigger, only a single ~21-nt interfering RNA is produced using shRNA/amiRNA. Although ...evaluated for their gene silencing efficacy, thereby requiring a substantial time investment. Once the target...widely implemented in plants, such as in the model flowering plant Arabidopsis thaliana (Li et al., 2013; Zhang...Gong B-Q, Guo Y, Xu L, Zhang X-N, Li J-F (2018) Engineering Artificial MicroRNAs for Multiplex Gene Silencing...

  11. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...enables researchers to silence or incite neuronal firing and study subsequent effects on behavior. The system...activation of light sensing proteins. Furthermore, during the first 2 weeks of life, larvae already exhibit...the cell membrane when exposed to light. Neuronal firing depends on these charged particles and whether ...said to be hyperpolarized. The chloride pump NpHR brings in negative chloride ions which hyperpolarize the...functions as a neuron “off switch.” Because of the differing peak absorption wavelengths and light intensity...

  12. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...thought leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters...organization what do you think of? Maybe the term brings to mind a social service organization like the ...believes there is strength in diversity and in empowering team members to take initiative and give their...our mission to accelerate research with easier sharing and collaboration. Joanne and all the Addgenies...

  13. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    ... project. Dissemination of the knowledge gained during this period occurs infrequently and rarely makes... wondered how we could make the insights gained during this time more visible and thereby have a positive...reviewers of your expertise and insight. We recommend sharing ReFigures on your Linkedin and ResearchGate profile...Please support ReFigure by creating ReFigures and answering this 2 minute survey! You can also find ReFigure...science at We Share Science Learn more about Data Sharing Resources at Addgene.org Deposit Your Plasmids...

  14. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ... the importance of leaving room for serendipity during your PhD studies and offer two simple steps to ...you to discussions beyond your immediate field, fostering a diverse range of perspectives and sparking innovative...capture fleeting thoughts and connections that emerge during moments of inspiration. Additionally, journaling...enrich the depth and creativity of your work, while offering mental health benefits by encouraging activities...How to Lead a Great Meeting Form Your Own Peer Mentoring Group...

  15. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type
    Blog Post
    ...used to spend my days (and often my nights :D) engineering E. coli to produce biofuels in Pamela Silver’...enterprise or that there weren’t some pretty hefty bumps during my graduate training. I particularly think that...and interesting than that will simply get people ordering plasmids. The need to keep content coming also...that I thought about careers outside of academia during my graduate training. PhDs have many roles to play...different fields. If you start thinking about preparing yourself for a career while still in graduate ...

  16. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...my recommendations on some ways to get started sharing your science with the community. Some of them you... up your own twitter feed or talking about and sharing some science on the one you already have. Read ...University. At Addgene we're committed to scientific sharing – both your plasmids and your research stories....  sharing your science... scientific sharing...

  17. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...on this topic), for this second entry in the “Mentoring for Scientists Series”, let’s focus on how to ...School researcher Connie Cepko to learn about her mentoring style. How can I find out if an advisor is a good...have good observations and be less inhibited in sharing concerns. Ask alumni of the lab. Most labs have...Depace's Talk on "Precision and Plasticity in Mentoring" Don’t ignore warning signs If lab members, neighbors...a Good Mentor?" and 6 More FAQs About Science Mentoring ...

  18. RUBY-Red Siliques

    Type
    Blog Post
    ...gene expression have similar drawbacks, often requiring the destruction of plants or tissue for GUS staining...show the effects of the mutation. But ¼ of the offspring from these plants will be green, containing the...removed to prevent further editing, then green offspring can be selected instead of red ones. Other innovative..., Sun, H. et al. A reporter for noninvasively monitoring gene expression and plant transformation. Hortic...Additional resources on the Addgene blog  CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis...

  19. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    ...peer-review is a touchstone of the scientific method, ensuring that published work meets the accepted standards...or omission from the literature. I think that delivering constructive critique can help your colleagues...following morning - as usually deadlines are pressing. During this second read, I pay more attention to details...passionate about open science, BIG data analysis and sharing whatever she knows with whoever cares to listen...the Addgene blog Find blog posts on scientific sharing Get tips on writing a review article Resources...

  20. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...lamenting the viral production process while still ordering viral vectors. Virus production can be a frustrating...experiments they’re interested in faster by producing, titering, and testing viral preps for them. At the same... service: "I'm excited that Addgene is now offering virion preparations to the scientific community...involving CRISPR and so we also have lentivirus for delivering control shRNAs, GFP, and rtTA from the Tet-on...continue to develop it. More communication and sharing will only help research advance. Remember: productivity...

  21. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...dopamine affinity, with 113050 offering high apparent affinity, and 113049 offering medium apparent affinity...target photostimulation of axons and dendrites on neighboring neurons. We have also packaged hChR2(H134R) plasmids...norepinephrine Neuroscience research relies on accurate monitoring of neurotransmitters and we are excited to offer...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  22. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...our eyes open for any new technologies that will bring about the next revolution in biological research...solution. 5. Cpf1: A New Tool for CRISPR Genome Engineering While the vast majority of CRISPR plasmids in...DNA, Addgene Science Communications Intern Mary Gearing explains how Cas9 homolog, Cpf1, may make it easier...difficult to use with CRISPR as the cargo. Mary Gearing explains some of the clever ways to successfully...validation process for CRISPR mediated genome engineering. 10. Plasmids 101: Control Plasmids Need help...

  23. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...requires an immense amount of self-knowledge and an unwavering devotion to seeing your ideas become something...CEO of miLab, a Boston-based startup focused on empowering early-stage scientists. You can think of miLab...generation companies.  We launched in the early spring of 2015 and since that time have received about..., including one led by a talented biomedical engineering team from MIT.   I would like to share my own... are everything and you want to leave people remembering your potential as an entrepreneur, not your credentials...

  24. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...advancements include work exploring far-red-emitting dyes in bacterial imaging, offering solutions to overcome...Photostability, the ability to retain fluorescent intensity during exposure to light, is another factor to consider...wavelengths, as this is limited by the filter cubes and filtering settings of the equipment. Systematic evaluation...facilitate clear differentiation between multiple dyes during analysis. Image resolution is impacted by several...Bhattacharya, K., Pyrgiotakis, G., Zhang, Z., Purington, E., Gramlich, W., Grondin, Y., Rogers, R., Bousfield...

  25. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... 2009), measuring intracellular calcium (Miyawaki, Griesbeck, Heim, & Tsien, 1999), monitoring kinase ... visualizing structural changes in the ribosome during protein translation (Ermolenko et al., 2007). All...acceptor molecules are targeted nearby (such as a neighboring house on the same street as the donor), then ...amber i.e. UAG) engineered into a desired site. During translation, the novel tRNA/unnatural amino acid...Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby enabling calibrated FRET distance...

  26. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ... when scientists bring biases to research and don't collaborate with others to bring diverse perspectives... rules all, the books center around a cast of endearing characters who decide to take matters into their...dueling magicians in the 1800s in England who help bring back actual magic (which had been lost for an age...a book club would force me to read some books exploring new genres, new authors, and new themes. A book...

  27. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    ...roles in fluorescent protein development by openly sharing their resources with the research community. To...protein abundance, possibly due to misfolding. But measuring variants one at a time is time consuming and unscalable...use the sensor to answer the question of whether firing of serotonergic neurons (measured using a red calcium...sensor in sleep-related studies and in assays for measuring human serotonin transporter activity. Unger also...dynamics with the TIMER growth rate bioreporter. During protein maturation, the bioreporter spontaneously...

  28. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...a producer cell line like the widely used 293T. During vector production, assembled AAV vector particles...released from the producer cells into the supernatant during the production phase. However, this has not been...at the AAV vectors that are released from cells during production (Maguire et al., 2012). They saw that...: PMC4104587. György, Bence, et al. "Rescue of hearing by gene delivery to inner-ear hair cells using ...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  29. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...recombination-mediated genetic engineering (find more information on bacterial engineering using CRISPR on our blog..., their interactions and information processing during this condition is crucial. Plasmids expressing ...interactions. Combining synthetic biology with engineering: glowing bacteria and a fish robot to detect ...interesting combinations: biomedicine and astrobiology. During the last 11 years he has studied various aspects...

  30. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ... HeLa cells?), genome engineering and stem cells, and the difficulty of sharing banked iPSCs across international...years, I've been looking forward to seeing how the sharing of his OSKM factors have affected the stem cell...paper as "life changing". Dr. Yamanaka himself, during his keynote address, appeared almost incredulous...into a pig. In stark contrast to recent genome engineering technologies, stem cell techniques are neither...

  31. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...for various scientific or engineering applications.   References Refactoring and optimization of light-switchable...photobiology: why use light? In synthetic biology and bioengineering, scientists seek to directly probe and build... publication at Addgene. Synthetic biology: engineering Escherichia coli to see light. Levskaya A, Chevalier... Mary Gearing...

  32. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...to the interview here! Doench majored in history during his undergraduate work at Hamilton College. When...that was open ended... it was actually something bordering on research, rather than 'here’s the protocol,... He first heard about RNAi at his MIT interview during a lecture given by Phil Sharp. After a few rotations... easier for people everywhere…. the nonprofit, sharing ethic that Addgene has behind it is something that... sharing...

  33. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...job. How can you incorporate the skills developed during graduate school into your resume? How can you stand...Here are a few things to consider when you're preparing your resume for a non-academic position: What ... current job as a Communications Coordinator. Wondering how you can develop more transferable skills? ...? See Developing Transferable Skills During Science Training and So You Think You have Skills.   Get Science...services, but some of the general knowledge about preparing a good resume, writing a concise cover letter,...

  34. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    ...interest in sharing, there will always be an interest in protecting intellectual property. Figuring out that...of Katz and Paratore onto the Board of Directors brings complementary experience and insight to the now... to the scientific community through Addgene’s sharing platform. Expanding further beyond the US and increasing...Internal Audit function and is responsible for bringing a systematic and disciplined approach to evaluating...

  35. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...components are still there in real life.  Certainly referring to any DNA within the context of a nucleus as ...methylation status at a double-strand break that needs repairing. They may be looking at chromatin at the level...! And if you study chromatin and want to start sharing your plasmids through Addgene, don’t hesitate to...Robert Kingston Thoru Pederson Phil Sharp Julie Ahringer Alexander Stark Bas van Steensel Richard Young...chromatin and the epigenome. If you have been considering some new techniques, the posts below may steer...

  36. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...” has me considering the microbiome more often than not). If you’re someone who’s considering getting ...Even if you're currently a synthetic biologist engineering E. coli metabolism, maybe you’ll be able to apply...because they provide a highly versatile means of delivering genes to many different cell types with minimal...Optogenetics, Neuroscience, and the Future of Neuroengineering Primer on Optogenetics Resources at Addgene.org...

  37. Clear and Accessible Writing

    Type
    Blog Post
    ...accessibility is removing barriers when we can and lowering them when we can’t. We know barriers exist in ...layers are blurred after centrifugation and the syringe needle is inserted bevel-up at the interface between...the 40% layer. To collect purified AAV, insert a syringe at the interface of the 40% layer containing the... iodixanol layer, with the bevel pointed up. A syringe at the top of the tube allows air to enter the ...the column so the virus will flow out the bottom syringe."Feel free to share your alt text in the comments...

  38. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...off-target cleavage should be an important consideration during any experiment. Sensitivity Cas9 sensitivity is... the Cas9 nuclease. This can be determined by measuring the indel frequency at a target site. A specificity...not be accessible by wild-type Cas9, due to its stringent PAM specificity. This can be especially important...PMCID: PMC6368452. Strecker, Jonathan, et al. "Engineering of CRISPR-Cas12b for human genome editing." Nature...Repurposing CRISPR-Cas12b for mammalian genome engineering." Cell discovery 4.1 (2018): 63. PubMed PMID:...

  39. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...Before I started writing for the Addgene blog, sharing Chemistry Cat memes was how I used social media...professionally, and 2) I needed to start using Twitter. Sharing Chemistry Cat memes use to be how I used social...conference, whether you are attending in person or monitoring that conference via social media. The podcast...Twitter is a tool worth using, but you might be wondering how to get started. This blog post outlines 10...unfollow those who do not capture your interest during that week.” You can also find Dr. Knoepfler on ...

  40. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...genome replication, repairing mismatched basepairs or small indels that occur during DNA synthesis, and...restriction enzymes are one half of naturally occurring restriction modification systems that prokaryotes...DpnI is often used for site directed mutagenesis. During this process, incorporation of a desired mutation...methylation Finally, you can control methylation by altering your choice of bacteria. For example, if you must...
Showing: 361 - 400 of 1006 results