Addgene

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Bringing Sustainable Practices to the Lab: Innovations

Type

Blog Post

...approach to lab waste is something we all need to do. Remember, it was only 50 years ago that we routinely dumped...
CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

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Blog Post

...This post was contributed by guest blogger, member of the Addgene Advisory Board, and Associate Director...
Seeing Red: Simple GFP Photoconversion

Type

Blog Post

...has a few more secrets - during a collaboration, members of Maureen Hanson’s and Rima Menassa's labs made...
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

Type

Blog Post

...more impactful by reaching more scientists. Many members of the scientific community support open sharing...
2023 and 2024: Reflections and Looking Ahead

Type

Blog Post

...integrins, will be released early this year. In September, we released the fourth edition of our Plasmids...
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

Type

Blog Post

...can be found in the following video: Please remember that the accuracy and reliability of our search...
A Better Way to Get Customer Support: The Help Center

Type

Blog Post

...Center Find Plasmids for Your Research Become a Member of the Addgene Community ...
Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

Type

Blog Post

...systems described in companion papers in Science in December were developed as an answer to that question. ...
New CRISPR Web Resources and #12DaysofCRISPR Recap

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Blog Post

...(plus bonus podcast with Addgene Advisory Board member Alex Chavez) Day 11: Pooled libraries: Genome-Wide...
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

Type

Blog Post

...offered by eScience Labs. “We work with faculty members to make sure the courses encompass what they want...
SunTag and Fluorescent Imaging

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Blog Post

...for single molecule imaging, finding that plasma membrane-targeted CAAX-SunTag was 18-fold brighter than...
Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

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Blog Post

...that allow charged particles to cross the cell membrane when exposed to light. Neuronal firing depends...
Hot Plasmids and Viral Preps - July 2021

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Blog Post

...function in these systems. As a part of this effort, members of the Rawlins Lab have compiled a new toolkit ...
Hot Plasmids - October 2022

Type

Blog Post

...photoactivation of HcKCR1 in mouse neurons. D) Membrane voltage traces of action potentials in a HcKCR1...
Understanding GPCR Signaling Using TRUPATH

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Blog Post

...They first assessed the activation of different members of the transducerome for both well-studied and ...
Oh, The Places You Can Go: Careers in Science Communication - Product Development

Type

Blog Post

...coordinator. With the guidance of one of the faculty members, I helped grad students who were teaching for the...
Addgene at the ASM Microbe 2016 Conference

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Blog Post

...communication through art. Addgene Advisory Board member Siouxsie Wiles gave us a fascinating and fun look...
Management for Scientists: What Makes a Good Manager Anyway?

Type

Blog Post

...does best to identify the work style of each team member) Need challenging work, opportunities to pursue...
Choosing Your Perfect Empty Backbone

Type

Blog Post

...expression system and has its advantages; however, remember that you will need accessory packaging vectors...
10 Ways to Share Your Science!

Type

Blog Post

...right track if they start asking you questions. Remember that people like a good story and science involves...
Plasmids 101: Antibiotic Resistance Genes

Type

Blog Post

... EtOH Polymyxin B polypeptide Alters outer membrane permeability Bactericidal 10-100 ug/mL Tetracycline...
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

Type

Blog Post

...and is used by scientists around the globe. As a member of the Brassicaceae family, Arabidopsis is related...
CRISPR Challenges: Standardization and Homology Directed Repair

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Blog Post

...Enhanced CRISPR Genome Editing Chris Richardson and members of Jacob Corn’s lab describe how a better understanding...
The Strength of Story Telling

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Blog Post

...of Nature magazine. Humans are built to enjoy, remember, and listen to narratives — take advantage of ...
Using Addgene's New Viral Service for Your Research

Type

Blog Post

...communication and sharing will only help research advance. Remember: productivity is infectious! Additional Resources...
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

Type

Blog Post

...successfully track nuclei in a developing zebrafish embryo over an 80 minute time course. By stitching together...
Single-cell tracking of lineage and identity with CellTag

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Blog Post

...CellTag to track the direct reprogramming of mouse embryonic fibroblasts (MEFs) into induced endoderm progenitors...
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

Type

Blog Post

...sequence was “optimized” so that it more closely resembles that of the host organism, it may express protein...
Summer SciComm Series: Master of SciComm

Type

Blog Post

...communication is an equally fruitful endeavor for those embarking on the route towards academia. Many of those entering...
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

Type

Blog Post

...my song about our paper recently published in @nchembio Title: Bye bye L-arabinose drive. Dedicated to...
Antibodies 101: Chimeric Antibodies

Type

Blog Post

...reactive to a certain isotype will bind to all members of that group. If you use two antibodies that are...
Viral Vectors 101: Transductions

Type

Blog Post

...specific viruses available to choose from. And remember, we have a selection of ready-made viruses as ...
Summer SciComm: Science Storytelling

Type

Blog Post

...” and that’s a very valid critique! But do you remember earlier when I said that even when we know the...
Donations from Addgene to Yield Answers for Rare Disease Researchers

Type

Blog Post

...play a role in many cell processes involving cell membrane dynamics. When they learned there was a disease...
Don’t Wait To Launch Your Health Science Startup

Type

Blog Post

... are everything and you want to leave people remembering your potential as an entrepreneur, not your credentials...
Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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Blog Post

...of interest into the FP-SEC vector using Gibson assembly. This construct will serve as the repair template...
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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Blog Post

...J. et al. Designing and using RNA scaffolds to assemble proteins in vivo. Nature protocols. 7(10) 1797...
Plasmids 101: NGS Quality Control for Pooled Libraries

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Blog Post

...libraries. Here are the key points you’ll need to remember when undertaking library amplification and sequencing...
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

Type

Blog Post

...polling feature or the chat box. One key thing to remember regardless of the type of session you’re presenting...
Scientists Map the SARS-CoV-2-Human Interaction Network

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Blog Post

...shipped out of the Krogan lab at UCSF. Our team members (shout out to Margaret Soucheray, Jeff Guo, Paddy...
Summer SciComm Series: A PhD in Science Communication

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Blog Post

...actually he drives better after a few drinks. A family member approached me for advice on her baby’s health but...
DAP arrays for multiplex gene editing

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Blog Post

...Typically, a single array encoding the tandemly assembled tRNA-gRNAs is placed downstream of a lengthy promoter...
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

Type

Blog Post

...is a science writer, former scientist, and proud member of Crowdfight. He is currently based in Spain and...
The MTA at Addgene

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Blog Post

...transferring! Many thanks to our Tech Transfer team members Maura Coughlin, Darcy Morse, and Matt Takvorian...
Viral Vectors 101: Inverted Terminal Repeats

Type

Blog Post

...ITRs in the plasmid backbone and do not properly assemble, but these sequences can sometimes be removed ...
Trading Spaces - Visiting Scientist Edition

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Blog Post

...trainee in your new lab, so make a good impression! Remember that your host is going out of their way to help...
K. phaffii: Rising to the Occasion

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Blog Post

...proteins. Peroxisome studies Peroxisomes are small, membrane-bound organelles essential for sequestering certain...
Networking Follow-up: I Met a Person. Now What?

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Blog Post

...Kamen's LinkedIn network! The connections between members of her network give rise to a starfish shape. ...
What Good Citizenship Can Do for Reproducibility in Science

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Blog Post

...including Cell Metabolism, Cell Reports and Joule. In September 2017 she moved away from direct editorial work...
Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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Blog Post

...investigating DNA repair pathways. Additonal Corn Lab Members who worked on this publication: References...

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