...with fluorescent derivatives of its substrate trimpethoprim (TMP-tag) (Miller et al., 2005). Although this...Benzylguanine (BG) Many Easy eDHFR 159 Trimpethoprim  (TMP-tag) Few Easy HaloTag 295 ... V. W. (2005). In vivo protein labeling with trimethoprim conjugates: A flexible chemical tag. Nature ...

...This post was contributed by guest blogger Greg Lohman, a biochemistry researcher at New England Biolabs...joining for recombinant plasmid construction (restriction cloning), adaptor ligation for next generation... can be achieved through a combination of the intrinsic selectivity of the ligase active site and careful...

...systems are less popular in the lab. This can be attributed to the multi-Cas effector, Cascade. Developing...Developing tools using a Cascade complex is tricky, as you need to ensure every unit of the complex expresses... Tolstoy, I., Johnson, D. B., Koonin, E. V., & Quatrini, R. (2021). Evolution of Type IV CRISPR-Cas Systems...

...This post was contributed by guest blogger Stephanie Hays, a researcher at the University of California...age live in a state with at least two abortion restrictions that conflict with scientific evidence. We need...promote harmful policies that benefit polluting industries.In March, three climate scientists and an economist...

...fluoresce, and then passing the cells through an electric field to deflect them into appropriate containers...understanding the key roles of immune cells in triggering an immune response. New fluorescent proteins ... PubMed PMCID: PMC5024581. 15. Celli, Susanna, Béatrice Breart, and Philippe Bousso. "Intravital two-photon...

... This post was contributed by guest blogger Fredrik Wermeling, leader of a research group at the Centrum...to ensure that each gRNA has a good chance to contribute to the screen. In the end, this results in an...could majorly accelerate progression into clinical trials. Secondary screens  If you have generated a list...

...audiencia internacional, incluido trabajando con distribuidores de todo el mundo para ayudar a aumentar el ...international audience, including working with distributors across the world to help increase global access...

... Addgene will then apply for the license for distribution to the requester’s organization. A non-refundable...for rabies materials may be required for some countries and may not be required for others. You will be...

...of all our depositors here at Addgene — your contributions make the repository what it is. So we’re thrilled...crystal structures demonstrating “the structure is strikingly similar to the design model at atomic resolution...

... to develop resources for your community! To contribute plasmids made in your lab, see www.addgene.org... a colleague who would benefit from our free distribution services for their popular constructs, why not...

...will vote on the entries, select up to three winners, and reveal the winning entries on Twitter and Instagram...

...tips you give to every new lab mate? Do you have a trick to get the highest yield out of your minipreps? ...love to have you write for us! Click Here to Contribute to the Addgene Blog Possible guest topics Some...

...help Addgene advance - plants for trimmed down protocols, distribution of new materials (check out our ...

...AAV9 Biosensors Patriarchi New viral service pAAV_hSyn1_nLightR AAV9 Biosensors Patriarchi New viral service...

... of the data, including total requests, total countries represented by requestors, year of first reagent... when analyzing and sharing your own depositor metrics!  About the Author Joachim Goedhart is an assistant...

...Heijmans There were many science-themed submissions: string lights hung up in the shape of RNA, light-up neurons...ladiesinlabcoats used a pipette tip box and microcentrifuge tubes to make Eppi the red nosed reindeer. ...

...’ve enjoyed seeing scientists sharing their contributions on social media as well the excitement amongst...community. For example, we started a new service distributing ready-to-use viral preps in 2016 and we just...

...people you should talk to if ruminating the trials and tribulations of the modern PhD program. Don’t be fooled...

...Executive Director, Joanne Kamens. As a regular contributor of careers blog articles, Joanne had plenty of...prizes were given out during the lunchtime pizza and trivia rounds, Senior Scientist Joel McDade’s presentation...

...increasingly moved online, surely times have changed. Altmetrics.org calls to expand our view of what impact looks...like to measure yours? More Reading Fenner M. "Altmetrics and Other Novel Measures for Scientific Impact...

... be tested carefully to ensure it retains the attributes of the native protein. Since this process takes...In contrast, Flag and HA tags had especially detrimental effects on cell size and growth rate, indicating...

...and listens in roughly equal measure, keeping contribution short and sweet. One key to this is ensuring...flow so everyone knows what is going on and can contribute. Members periodically break, go exploring outside...

...the COVID-19 plasmids first became available: distribution was limited to academic scientists. Expanding...and archiving system allowed us to handle and distribute such a large deposit. Once the project is complete...

...scientific sharing as the scientific community strives to understand COVID-19. While the specific motivations... community The team behind Crowdfight COVID-19 strives to match up scientists across fields with different...

...approaches to control transgene expression by restricting expression to genetically defined cell types....can then be targeted with optical stimuli. The restricted light exposure to a specific area in combination...

... muscular atrophy (SMA)." European Journal of Paediatric Neurology 19 (2015): S64-S65. Additional Resources...Donating Plasmids to Researchers in Developing Countries with Seeding Labs Resources on Addgene.org Find...

...she started things off, “We are all capable of contributing to change.”  Check out Joanne's Reddit AMA ...topics were Training, Structure of the Workforce, Metrics and Incentives and Funding.  I can’t wait to see...

...    sdAbs in research These advantages have contributed to sdAbs becoming valuable tools for diverse ... approved in 2018, with many others in clinical trials (Jin, et al. 2023). In addition to therapeutics...

...able to understand what metrics would be useful and how to organize the metrics in a digestible manner....

... functions they may induce?  Fc receptors are intriguing from a therapeutic standpoint. Engineered monoclonal.... (2008). The immunoglobulin constant region contributes to affinity and specificity. Trends in Immunology...

...channelrhodopsins (KCRs), from a fungus-like protist Hyphochytrium catenoides (Govorunova et al. 2022). The authors...not just because of the title!) The Myc-tag is a string of 10 amino acids, EQKLISEEDL, that can be incorporated...

...Addgene has distributed over 100,000 CRISPR plasmids. But that’s not our only job - we strive to also give...

... tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate...PMID: 4403305. PubMed Central PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging ...

...Addgenie Mary Gearing contributed to the content of this article. Biosensors (‘biological sensors’) are...separated by CaM and M13. When calcium binds, it triggers CaM/M13 interaction that increases FRET transfer...

... experiments to the PIs. Remote meetings can be tricky, and it takes extra effort to make sure everyone...everyone in the meeting has an equal opportunity to contribute. Using video conferencing tools like Zoom or ...

...This post was contributed by the Open Repository of CRISPR Screens. Imagine you’ve just discovered that...open access and available without any licensing restrictions.    As CRISPR technology continues to develop...

...for sale, and photos of the Agar Art competition entries were displayed in a gallery on the second floor... to find out more about our free deposit and distribution service! Did you see a must-have plasmid during...

...RFP, no FP). The vectors each contain two SfiI restriction sites with different overhangs depending upon...Cancer Center presented results from a clinical trial using the Sleeping Beauty transposase as a nonviral...

...entire meeting, Monday's talks alone were worth the trip. Dr. Dana Carroll's excellent keynote address was...genome engineering tools – both deposited and distributed – have changed so dramatically in the past 4 ...

...one step involves boiling the glass powder in 50% nitric acid, but I never let that stop me. I only had ...glass slide using only surface tension.  It's a bit tricky to pour the right amount so the agarose doesn’t...

...to the antibiotic that remains intact. Tips and tricks from the bench Use fresh stocks. Most antibiotics... Marcy Patrick...

...mL Amp instead of 100 μg/mL Amp), or using more nutrient rich media can improve growth and DNA yield. Test...determine if your sample has recombined. Choose a restriction enzyme that will give you different band patterns...

...fragments that recognize the endogenous IgGs (Lu and Partridge, 1998). After washing away unbound fragments, ...https://doi.org/10.1369/0022155413511620  Lu QL, Partridge TA (1998). A New Blocking Method for Application...

...HEKs have an unusual ploidy and are considered hypotriploid: most chromosomes are represented by two to ...cells may be particularly useful due to their intrinsic properties. HEK-293 cells are tumorigenic, ...

...This post was contribued by guest bloggers Aline and Benjamin Glick from SnapGene. SnapGene was created...don’t use SnapGene yet, you can download a free trial. If you notice that a feature is missing from our...

... This post was contributed by guest blogger Jack Leeming Editor of Naturejobs, part of Nature magazine...dialogue specifically about the project and contributions, rather than getting defensive or getting into...

...This post was contributed by Richi Sakaguchi from Kyoto University, and Marcus N. Leiwe and Takeshi Imai...copies per cell per color, following a Poisson distribution. To overcome the limited expression levels of...

... dark matter and so the lab organized a sampling trip to Yellowstone National Park (YNP). Extremophiles... another Quake Lab member joined for half of the trip. Preparations ahead of time included contacting ...

...fantastic fodder for science communication. The trick is to find one or two roadblocks that sparked creativity...makes them want to know more. This could be by outright stating the big-picture problem, by asking your...

...suggest topics you’d like to see on the blog or to contribute ideas for your own guest posts by e-mailing us...generate knockouts, but how does it work? Guest contributors David Wyatt and Dale Ramsden from UNC Chapel...