We narrowed to 1,092 results for: addgene
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TypeBlog Post...on the Addgene blog Learn more about plasmid sharing during COVID-19 Resources on Addgene.org Find ...examine a dataset of Liu lab plasmid requests from Addgene, as a proxy for global activity in our particular...over 6 years for 35 papers (data was provided by Addgene on 5/26/20 and contains requests from 5/22/14 to...
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Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post...more. Being that this is the Addgene Blog, I’ll, of course, mention Addgene as a source of sequenced and...event, I gave my own talk on reproducibility at Addgene and here I summarize what I learned both in preparation...31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid repository 49:45 0 1:18:45...viral vectors. When working with a plasmid from Addgene, you can be confident that you’re using the actual...hosted by individuals and by organizations like Addgene (a good example is our series of blog posts on ...Reproducibility at Protocols.io Additional Resources on the Addgene Blog FPbase: A new community-editable fluorescent... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post...1 are available at Addgene. Table 1: List of pCXLE episomal plasmids, their Addgene ID numbers and the...Field Sign Up to Write for the Addgene Blog! Resources on Addgene.org Browse Our Stem Cell Collection... diseases in vitro. Find stem cell plasmids at Addgene Many sources of adult somatic cells have been ...reprogramming factors they encode Episomal Plasmid Addgene ID# Encodes pCXLE-hSK 27078 SOX2 and KLF4 pCXLE-hUL...PMCID: PMC2922531. Additional Resources on the Addgene Blog Stem Cell Models for Disease & Open Science... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...Addgene recently attended and sponsored the Future of Research (FOR) Boston Symposium 2015. I personally...fun and surprisingly exhausting experience. As Addgene believes many of the topics discussed during the...are many companies and organizations (including Addgene), that need people who are data oriented, scientifically...skills beyond those required at the lab bench. At Addgene we try to do our part in boosting awareness of ...problems within the research environment itself. At Addgene we hope that all parties involved in making academic...Publications of Interest Can Be Found Here Resources at Addgene Read David Riglar's FOR Boston 2015 Blog Post... -
Bringing Sustainable Practices to the Lab: Easy Action Items
TypeBlog Post...resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Learn...resources by sharing plasmids Find plasmids at Addgene ...approaches for lab sustainability Resources on Addgene.org Sign up for our quarterly newsletter Help other... -
Grad School Advice Part 2: Building Community
TypeBlog Post...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. Additional...Additional Resources on the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research...Process Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your... -
Grad School Advice Part 1: Picking a Lab and a Project
TypeBlog Post...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast. Additional...Additional Resources on the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research...Process Browse our Career Posts Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your... -
Fueled by Coffee at #SfN14
TypeBlog Post... event – all while keeping the party going! At Addgene, we’ve set-up an exhibitor booth in order to help...all the words of support from scientists using Addgene in their labs. And it has been exciting to learn...McGovern Institute for Brain Research) – and an Addgene depositing scientist – stop by our booth to say...by booth #3431 if you’re at #SfN14 and meet our Addgenie crew and sign-up for a free plasmid! Also check... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...Conjugation, and Transfection Resources on Addgene.org Addgene's Synthetic Biology Collection...Here at Addgene, we often refer to plasmids as lab or experimental tools. They certainly are very handy...deposit the many plasmid tools available in the Addgene repository. To learn more about some other common...other Plasmids 101 posts, and keep an eye on the Addgene blog as scientists continue to explore Mother Nature.../07388550490904288 Additional Resources on the Addgene Blog Plasmids 101: Antibiotic Resistance Genes... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog Post...corresponding portions of the Zika virus genome with Addgene to facilitate rapid sharing of these tools with... community. Find the Zika Sensor Constructs at Addgene As we report, we have embedded this next generation...27160350. Find Plasmids from the publication at Addgene 2. Green, Alexander A., et al. "Toehold switches...e15272. PubMed PMID: 27090089. Resources on the Addgene Blog Read other Synthetic Biology Posts Listen...Your Next Experiment Deposit Your Plasmids with Addgene ...Used to Study Dangerous Viruses Resources on Addgene.org Check out All the Plasmids from the Collin's... -
Fluorescent Protein Guide: Biosensors
TypeCollection...localize to cellular compartments or organelles. Addgene’s database includes many fluorescent biosensors ... the Promega Plasmid Collection More Resources Addgene Blog Topic: Biosensors Optogenetics Plasmids Viral...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!... -
Viral Vectors 101: Transductions
TypeBlog Post...Viral Services Resources on the Addgene blog Viral Production at Addgene Viral Vectors 101: What is a Virus...Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral... Is there an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...and tips Resources on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols...interest. While the VGS process isn’t new to Addgene, we are excited to share the procedures and Python....ymthe.2017.04.001 Additional resources on the Addgene blog Read all of our viral vectors related blog... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post...out full plasmid sequencing at Addgene Additional Resources on Addgene.org Example plasmid analysis powered...annotation gaps in the plasmids available through the Addgene website. In addition, two initiatives are in the...University of Chicago. He was an early depositor at Addgene, and conceived SnapGene because he needed it for...post-launch iterations. Additional Resources on the Addgene Blog Read about our SnapGene powered plasmid maps...feature database. Powering the plasmid maps on Addgene.org with SnapGene Server is useful because it helps... -
Cultivating Community Science at BosLab
TypeBlog Post... at Addgene.org Order Plasmids from Addgene! Find Free and Open Educational Resources at Addgene! ...microbes. We’ve ordered a few yeast plasmids from Addgene and are eager to do our first yeast transformations... @ZebraElement Additional Resources on the Addgene Blog Experimenting in an Open Source Lab: from... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post... al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova et al., Methods, 2019. PubMed PMID:...difficult. Find SpCas9 and Target-AID-NG plasmids at Addgene! Nishimasu H et al., Science, 2018. PubMed PMID...research and innovation. Find MerMAIDs plasmids at Addgene Opperman J et al., Nature Communications, 2019....plasmids at Addgene Kim et al., Nat Methods. 2019, PubMed PMID: 31235883. ... Various Addgenies... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog Post...plasmids from this publication at Addgene. Additional Resources on the Addgene Blog An “elegans” Approach...PubMed. Find plasmids from this publication at Addgene. Other strategies for CRISPR/Cas9 editing in ...PubMed. Find plasmids from this publication at Addgene. Scalable and versatile genome editing using ... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog Post...Twitch calcium sensors, which are available at Addgene, can be used for ratiometric in vivo imaging. Griesbeck...the first of its kind. “I hope by depositing at Addgene, people will start using them,” Merkx said. “For...(PubMed) Interested in More Hot Plasmids from Addgene? Check out our Hot Plasmid page, where we highlight...some of the popular & newly available plasmids at Addgene. If you're interested in plasmids for in vivo imaging... -
Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service
TypeBlog Post...experiment? What if after receiving the plasmid from Addgene you could directly start your digest, PCR, or transformation...Suggestion Form. Additional resources on the Addgene blog Browse blog posts from our Plasmids 101 blog...the various ways to quantify DNA Resources on Addgene.org Learn about our other beta projects Read our... -
CRISPR Guide Update
TypeBlog Post...Addgene is proud to present our updated CRISPR Guide! The CRISPR Guide helps scientists understand how...offer. Happy CRISPR-ing! More resources on the Addgene blog PRIME Editing CRISPR 101: Cas9 vs the Other... -
What's New in CRISPR - May 2019
TypeBlog Post... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by... -
What's New in CRISPR - Spring 2019
TypeBlog Post... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...please let us know! Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by... -
Science Communication Snapshot: DayCon 2015
TypeBlog Post... Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate...terms of cost and content level. Sponsorship from Addgene and Harvard Integrated Life Sciences (HILS) made... do you share your science with the community? Addgene would love to hear about your efforts to engage... -
Save Time and Money by Making Your Own Competent Cells
TypeBlog Post... perform over 100 transformations each week at Addgene, so we need to be cost and time efficient. We do...Preparation of electrocompetent cells (protocol) At Addgene, we use the Mix & Go! E. coli Transformation Kit...large number of transformations being performed at Addgene, this adds up to a significant time savings. You... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog Post...time on Addgene’s role in the rapid dissemination of these technologies, proposing that “Addgene, with ...has been a key driver of the technology.” As an Addgene vet of 12 years, I’m very familiar with the role...I discussed The State of Science with the five Addgene colleagues who read it with me, the consistent ...about many different things (part of the reason Addgene has been such a great fit for me!), the book was...Acknowledgements: I would like to thank fellow Addgenies Joanne Kamens, Marcy Patrick, Aliyah Weinstein... -
A Guide to Starting Your Own Journal Club
TypeBlog Post...want to pick a topic and have no one show up. At Addgene, we wanted to think more closely about the wealth...: Discussing next-generation sequencing at the Addgene bioinformatics journal club! What happens during...messaging but we as scientists often prefer data. At Addgene we rely on polls and surveys. A mix of closed (...most likely the primary drivers of frequency. At Addgene, we opted to start with one per month. When to ...own journal club! Additional resources on the Addgene blog Read our blog posts centered around scientific... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...suited for genome editing. Find Cpf1 Plasmids at Addgene Image attribution: Zetsche, Bernd, et ...27272384 Find plasmids from this publication at Addgene. 2. Kleinstiver, Benjamin P., et al. "Genome-wide...27347757. Find plasmids from this publication at Addgene. 3. Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker...26422227 Find plasmids from this publication at Addgene. 4. Makarova KS, Wolf YI, Alkhnbashi OS, Costa...nrmicro3569. PubMed PMID: 26411297 Resources on the Addgene Blog Catch Up On Your Cpf1 Background Read About...piggyBac Vectors in Genome Editing Resources on Addgene.org Check Out Our CRISPR Guide Pages Check Out MpAgo... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post...making it easier to construct targeting vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed ...systems The ready-made PITCh plasmids available from Addgene are perfect for expressing GFP from a given promoter...26678082. Find the plasmids from this publication at Addgene. 2. Dalvai, Mathieu, et al. (2015). “A Scalable...26456817. Find plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei Wei, and ...57. PMID: 26701308. Additional Resources on the Addgene Blog Brush up on Homology Directed Repair and ...for Your Experiment Additional Resources on Addgene.org Learn All about CRISPR on Our CRISPR Guide Pages... -
Biosensor AAV Preps
TypeCollection...Ready-to-use AAV available from Addgene's viral service encoding biosensor tools, including calcium sensors... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post... on Addgene.org Ready-to-use recombinant antibodies at Addgene Addgene's Antibody Guide Addgene's Antibody... combines a marker with a signal. For clarity, Addgene uses 'labeling' to refer to antibodies and 'staining...Find streptavidin plasmids and biotin plasmids at Addgene! Figure 1: Indirect detection of target...Antibody Plasmid Collection Resources on the Addgene blog Antibodies 101: Reading a Flow Plot Antibodies... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...Screens Additional Resources onf Addgene.org Check out Addgene’s newly updated CRISPR Guide! Find CRISPR...Activation Mediators (SAM) system available from Addgene. This system uses the dCas9-VP64 fusion commonly...others and accelerate the pace of research. We at Addgene hope to see the problem of in vivo CRISPR delivery... Find SAM plasmids used in this publication at Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli...packaging plasmids used in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S...PubMed PMID: 25716921. Additional Resources on the Addgene Blog Treating Muscular Dystrophy with CRISPR ... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post...about pseudotyping Resources on Addgene.org Find plasmids in Addgene's COVID-19 collection Find SARS-COV...Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing COVID-19 research. “I hope...j.cell.2021.03.013 Additional resources on the Addgene blog Browse all COVID-19 related blog posts Learn...-COV-2 pseudotype virus plasmids from Addgene ... -
New and Upcoming Viral Vectors - May 2020
TypeBlog Post...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...you pick the best AAV for your experiment. The Addgene viral service has now expanded to offer many of...These vectors listed below should be released at Addgene in the next 6 months, pending quality control. ...pAAV-mDlx-GCaMP6f-Fishell-2 Additional resources on the Addgene blog Read all of our viral vectors blog posts ...item is available for order please email help@addgene.org with the vector name and serotype you’d like ... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...coronavirus or COVID-19 research, please email Addgene at help@addgene.org with your order number and so it can .... Sign up for email alerts on the Krogan lab's Addgene page to get notified!) Attacking coronavirus, one...reasons, the QCRG is delighted to be partnering with Addgene, who will be taking over the distribution to expand... coronavirus related plasmids and resources at Addgene* Many thanks to our guest bloggers Manon Eckhardt... -
Plasmids 101: Blue-white Screening
TypeBlog Post... on the Addgene Website Choose the Best Molecular Cloning Technique for You Browse Addgene's curated ... Vectors for Blue White Screening Available at Addgene Are: pUC18 and pUC19 Let’s begin at the beginning...which rescue a lethal phenotype. Resources on the Addgene Blog Read Our Post on Common Lab E. coli Strains... -
What's New in CRISPR - December 2019
TypeBlog Post... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...more information. Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids by... -
Microbes: Look, Listen, and Tell
TypeBlog Post...way to visualize them. You can also “hear” them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented...PubMed Central PMCID: PMC5920530. Resources on the Addgene Blog Read Our Post on CRISPR Methods for Bacterial...bacteria Deposit Your Microbiology Plasmids with Addgene ...Science Communication using Twitter Resources on Addgene.org Check out Our Microbiology Resource Page CRISPR... -
Viral Vectors 101: Producing Your rAAV
TypeBlog Post... excellent quality, high titer rAAV (including Addgene!), but you can also generate rAAVs in your own ...details on the production process, check out the Addgene AAV Production protocol. Titering Once you’ve produced...details on the qPCR titering process, refer to the Addgene AAV Titration by qPCR Protocol. If you’re interested...interested in the ddPCR titering process, which is what Addgene uses, check out our ddPCR for AAV quantitation ...10.1089/1043034041361262 More resources on the Addgene blog Parts of the AAV Packaging PlasmidParts of...PlasmidAAV Variables That Matter AAV protocols on addgene.org AAV Titration by qPCR Using SYBR Green TechnologyAAV... -
Plasmids 101: Gateway Cloning
TypeBlog Post... attL-containing entry vector. Pro Tip: Addgene also has ready-made entry clones available for ...expression system in just one recombination step. Addgene’s ready-made entry clones can be used with a large...of the Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple Lentiviral Expression...deposited their own Gateway-compatible plasmids with Addgene. These can be used to express genes in a variety..., MA: Cell Press). Additional Resources on the Addgene Blog Try Out FastCloning Learn About Methylation... to Quickly Verify Your Clones Resources on Addgene.org Find Plasmid Cloning Protocols Watch Our Diagnostic... -
Droplet Digital PCR for AAV Quantitation
TypeBlog Post...Since Addgene began producing AAV vector preparations, we have used quantitative PCR to evaluate the ... qPCR can be challenging to get right. Here at Addgene, we use a linearized plasmid standard. However,...virus before adding your sample to a mastermix. At Addgene, we typically load 3 dilutions onto our ddPCR plate...PMCID: PMC5167268. Additional resources on the Addgene blog Read more about polymerase chain reaction... vector related protocols Learn more about the Addgene viral service ...of our viral vectors blog posts Resources on Addgene.org Learn our protocol for AAV titration by qPCR... -
Lentiviral Vector Uses and Overview
TypeBlog Post...design a viral vector system that could do both, Addgene depositor Didier Trono and collaborators turned...lentiviral packaging and envelope plasmids are two of Addgene’s most requested plasmids! Lentiviral particles ...plasmids are designed for lentiviral use, including Addgene’s top requested plasmid in 2015: lentiCRISPR v2,... terms of research impact and safety. Many of Addgene’s most requested plasmids are used with lentiviral...PubMed PMID: 19847206. Additional Resources on the Addgene Blog Your Lentiviral Plasmid FAQs Answered Learn...Screening Using CRISPR/Cas9 Additional Resources on Addgene.org Check out Our Lentivirus Guide Learn about Virus... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog Post...resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the Addgene blog Find...resources by sharing plasmids Find plasmids at Addgene ...Find lab tips for your research Resources on Addgene.org Sign up for our quarterly newsletter Help other... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post... More resources on addgene.org Addgene Protocols: General Transfection The Addgene Retrovirus Guide ...transduction. Most transfer plasmids available from Addgene were derived from MoMLV (Moloney Murine Leukemia...im1017cs31. PMID: 18432682. More resources on the Addgene blog Viral Vector Elements The Retroviral Life ...This post was written and updated by multiple Addgenies. It was originally published in 2018, in the Viral... -
Viral Vectors 101: Viral Applications
TypeBlog Post...have already been built and are available on the Addgene catalog – so check there before building one from...therapy—viruses could further your efforts. And Addgene is here to help! We offer a variety of viral vectors... Click Here to Find Viral Vector Resources at Addgene! References and Resources References Travieso...s41392-021-00487-6 Additional Resources on the Addgene Blog AAV: A Versatile Viral tool for Gene Expression...Editing to Primary Cells Additional Resources on Addgene.org Browse Our Viral Vector Pages Learn About Viral... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post...specifically how dry ice can affect virus from Addgene. Bottom line: there’s good news and there’s bad... results. Order Ready to Use Viral Preps from Addgene Measuring the pH of our samples: What we did To... recommend that once you receive your AAV from Addgene, you store the AAV at -80°C for 96 hours prior ... PMCID: PMC4420252 Additional Resources on the Addgene Blog Learn How You Can Use the Viral Service Tips...Your Lentivirus Preps Additional Resources on Addgene.org Check out the Viral Service Find Virus Protocols... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post...Immunotherapies Resources on Addgene.org Fine More Kits at Addgene Browse Plasmids from the Broad ... available to the scientific community through Addgene. These include 634 sequence confirmed mutant cDNA...and we’re proud to have worked closely with our Addgene colleagues to make these available to the scientific...Twitter @boehmjesse. Additional Resources on the Addgene Blog Learn About our Cancer Colleciton Pages Use... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...accelerate their work. After all, as we like to sayat Addgene, productivity is infectious. Curious which viruses...please)... Top Lentivirus: lentiCas9-Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools,...if you received lentiCas9-blast lentivirus from Addgene, you could use it in conjunction with gRNA lentiviruses...PMCID: PMC4486245. Additional Resources on the Addgene Blog Learn More About Lentivirus Learn more About...the Scoop on Our Viral Service Resources on Addgene.org Find Ready-to-Use Lentivirus Find Ready-to-Use... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...split base editors. Find the split base editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020...the Ace-mScarlet voltage indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed PMID: 31685893...Vasudevan et al., 2018. Find the CyanoGate kit at Addgene. Vasudevan et al., Plant Physiol 2018. PubMed PMID...Find CreLite at Addgene. Yen et al., bioRxiv 2019. DOI: 10.1101/823971. ... Various Addgenies... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog Post...scientific sharing will continue to grow, and we at Addgene appreciate our depositors for making their tools...29551272 Plasmids from this paper are available at Addgene. Yan, Winston X., et al. “Cas13d Is a Compact ...29551514 Plasmids from this paper are available at Addgene. Learn more about research at Arbor Biotechnologies...Biotechnologies. Additional Resources on the Addgene Blog: Learn about CRISPR RNA Editing with REPAIR Get tips...Cas13a (C2c2) Characterization Resources on Addgene.org: Find RNA Knockdown Plasmids Check out our CRISPR... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog Post...and thus transformation efficiency. Check out Addgene’s blog to learn about making your own competent ... as well as ready-to-use viral preparations at Addgene. For more information on viral vectors, including...starting any molecular biology experiment check out Addgene’s protocol page, which has both protocols and videos...PubMed PMID: 16357262. Additional resources on the Addgene blog Read all of our Plasmids 101 blog posts Check... blog posts on plasmid cloning Resources on Addgene.org Read our protocols for plasmid cloning Find ...