We narrowed to 535 results for: try;

1. The Power Behind NGS Plasmid Validation: seqWell

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   ...range of input concentrations (via proprietary chemistry). This turns out to be critically important for...

2. Trading Spaces - Visiting Scientist Edition

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   ...while experiencing a new city or maybe even a new country can also be exciting! Identifying a host institute...

3. Revamp Your Lab Meetings With Creative Virtual Collaboration

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   ...PhDs: Swimming upstream a career from academia to industry”. To date, aside from doing science, he has assisted...

4. Networking Follow-up: I Met a Person. Now What?

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   ...Follow your contact’s company on LinkedIn (or via industry newsletters such as FiercePharma), or sign up ...

5. Communicating Your Science With Help From ComSciCon

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   ...works as a data scientist in the entertainment industry.       Additional resources on the Addgene Blog...

6. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   ...87% mCherry protein knockdown measured by flow cytometry, respectively. Cas13d CRISPR array processing ...

7. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   ...Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike Proteins. In:...

8. Building and Validating Recombinant Antibodies: A Case Study

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   ...functional validation by immunofluorescence-immunocytochemistry (IF-ICC.) This method was chosen because ...

9. Antibodies 101: Monoclonal Antibodies

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   ...Ligand-induced Epitope Masking. Journal of Biological Chemistry 291:20993–21007. https://doi.org/10.1074/jbc.m116.736942...

10. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    ...Pathway Reference Set. These Gateway-compatible entry clones are the product of many years of work and...

11. Viral Vectors 101: The AAV Retrograde Serotype

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    ...reagents, AAV particles require receptor-mediated entry into the cell. This means that not all neurons and...

12. CRISPR 101: Non-Homologous End Joining

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    ...Genetics and Molecular Biology, the Dept. of Biochemistry and Biophysics and the Lineberger Comprehensive...

13. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    ...is. While communication styles in academia and industry vary drastically, he reassures that one can learn...

14. Plasmids 101: Yeast Vectors

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    ... multiple copies are required. yes   TRP1 L-tryptophan yes - Grow with 5-FAA. S. cerevisiae no  no TRP1...

15. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    ...potluck dinner. Invite guests from a lab, career or industry of interest – learn about their career paths and...

16. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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    ... Stefan Hell, who won the 2014 Nobel Prize in Chemistry for his contributions to super-resolution light...

17. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    ...that it is driven by academics, with support of industry partners, not the other way around. Sometimes,...

18. Celebrating 15 Years of Scientific Sharing

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    ...request from us but also by high school students and industry scientists as a universal resource for information...

19. Antibodies 101: Affinity Reagents

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    ...Antigen Binding Format. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.M407888200 Kwon, ...

20. Viral Vectors 101: An Introduction to AAV

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    ...the both ends and mutated ITR at the center of symmetry. After uncoating in the target cell nucleus, this...

21. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    ...and suggestions, and even to submit a guest blog entry of your own! References 1. Bernard, P. “Positive...

22. Developing Lab Management Software for Biology

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    ...If it’s barcoded, it should have a corresponding entry in your database. Enjoy your happy lab staff. ...

23. Plasmids 101: Cre-lox

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    ...-bp long asymmetric core spacer sequence. The asymmetry in the core sequence gives the loxP site directionality...

24. Working with Nuclear Receptors

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    ...adipogenesis and macrophage activation. Our mass spectrometry-affinity purification proteomic profiling of...

25. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...from the Frew lab consists of 91 building block Entry vectors and 5 lentiviral Destination vectors. These...

26. Adenoviral Vector Production and Troubleshooting

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    ...looking at absorbance at 260nm using UV/Vis spectrophotometry. An absorbance of at 260nm corresponds to...

27. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    ...Doug Warner on the right) has taught courses in biochemistry, molecular cell biology and genetics at Boston...

28. Hot Plasmids - October 2020

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    ...greater number of red cells as measured by flow cytometry. Cells grown in high IL-3 showed mostly blue fluorescence...

29. Corralling and Cornering the Goldilocks Antibody

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    ...compatible with the desired applications (such as immunohistochemistry (IHC) or western blot), and cost-effective...

30. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ... a patient’s blood), scientists can use mass spectrometry to determine the amino acid sequences of the...

31. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...   Confirming the gene/insert by aligning to NCBI entry or using BLAST.   Confirming tags and fusion proteins...

32. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    ...Regulation by Long Noncoding RNAs. Annual Review of Biochemistry. 2012;81(Volume 81, 2012):145-166. doi:10.1146...

33. Illuminating Epigenetics with A FRET Based Biosensor

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    ...like the potential devastation of the palm oil industry through epigenetic effects on the Cover of Nature...

34. Polymerase Chain Reaction Overview and Applications

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    ...Kary B. Mullis who later shared a Nobel Prize in Chemistry for his work. Since then PCR has become a standard...

35. Hot Plasmids and Viral Preps - May 2021

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    ...2020. Preger et al., Journal of Biological Chemistry 2020. https://doi.org/10.1074/jbc.RA120.01289 ...

36. Cloning Mammalian Cells with the Agarose Method

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    ...line, and its transfection efficiency. We often trypsinize the transfected cells on the day after transfection...

37. Fluorescent Proteins 101: Fluorescent Protein Timers

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    ...fiber bundle formation." Journal of Biological Chemistry 276.32 (2001): 29621-29624. PubMed PMID: 11408473...

38. PhD Applications After COVID

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    ...application cycle, two schools on opposite sides of the country had scheduled visitation days during my midterm...

39. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    ...AnemoneCnidopus Japonicus. Journal of Biological Chemistry 281:37813–37819 . https://doi.org/10.1074/jbc....

40. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    ...Jaenisch, R. and R. Young, Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming. Cell...

41. Anatomy of a Plasmid Page at Addgene

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    ..., so some of our older plasmids may not have an entry in this field. If you’ve deposited a plasmid without...

42. Plasmids 101: Secondary Nanobody Toolbox

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    ... single-domain antibodies.” Annual review of biochemistry 82 (2013): 775-97. PubMed PMID: 23495938. Additional...

43. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...those of their endogenous counterparts (by immunocytochemistry, for example), as this can also be perturbed...

44. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    ...s a very big question. Zimmer, a Professor of Chemistry at Connecticut College who has been teaching and...

45. Tips for arabidopsis transformation

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    ...Analysis of Plants’, Bioscience, Biotechnology, and Biochemistry, 71(8), pp. 2095–2100. doi: 10.1271/bbb.70216...

46. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    ...proteins,” ChIP-seq from Table 1. Because of DNA’s 3D geometry and the chemical properties of modified histones...

47. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    ...lab team takes over and prepares the sample for entry into Addgene’s repository. For long-term storage...

48. Your Lentiviral Plasmid FAQs Answered

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    ...such reagents as outlined by your institution or country. Have any other questions? Feel free to post them...

49. Neuronal labeling with Spaghetti Monster

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    ...protein of interest (POI), that facilitate immunohistochemistry (IHC) experiments with tag-specific antibodies...

50. Deciphering the Mysteries of Behavior with Viral Vectors

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    ...the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (2007): 1079. PubMed PMID: 17700574. Hare...