...transduction of Cas9-expressing (Rosa26-Cas9) hematopoietic stem cells with a lentiviral vector containing...LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in...G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential ...

...without permanently modifying the genome dCas9-activator (such as dCas9-VP64) gRNA(s) targeting promoter...promoter elements of target gene Many different activators exist, including the multi-plasmid SAM system. Select...activate or repress a target gene using dCas9-activators or dCas9-repressors, gRNAs should be targeted...

... interpret signals from these reporters. Lack anatomical context/spatial localization: In vivo imaging...years to clinically treat enteric infection and inflammatory bowel conditions. In these experiments, arg1...abdomen. arg1 signal, however, can be overlaid onto anatomical ultrasound imaging to show where the bacteria...

...department.” Caitlin does this by acting as a facilitator between her team, the sales department, and operations...business.” Caitlin’s advice for budding science communicators: “My recommendation would be to take any opportunity...education tools? Check out this Addgene podcast with educator and science rap mastermind Tom McFadden Get tips...

...scientists. Over the course of the class, the moderator discussed individual sections of a paper, the ...quizzes and discussion on that week’s topic by the moderators. The assignments, which consisted of reading ...course was breaking up into focus groups with a facilitator. In the focus groups, we were required to critique...

...passion, and dedication to sharing epitomized by the creators of these plasmids with the first annual Blue Flame...has “literally enabled sharing with 100's of investigators worldwide. Now everything from every paper is...Flame awardee Sophien Kamoun of the Sainsbury Laboratory in Norwich, UK: “We’re very pleased to distribute...

...plasmid. Parts shown in grey are optional cis-regulatory elements.   Key elements of an AAV plasmid...plasmid harbors non-coding sequences. Other cis-regulatory elements Next let’s talk about some of the elements...Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) forms a tertiary structure when...

...Willoughby   Using RUBY as a selection marker The creators of RUBY highlight its use in crop plants under... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...promoter with self-cleaving 2A site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al....

...Neurobiology has been working on genetically encoded indicators of calcium and other small molecules since the...imaging for beginners Griesbeck says his calcium indicators can be used in mouse, Drosophila, C. elegans,...his team is now applying it to improve upon an indicator they developed and published in 2010 for visualizing...

...to create gene deletions of important unknown regulatory regions in Pseudomonas and Klebsiella spp. The...Ibadan, Nigeriaunder supervision of Dr. Akindele Oluwatosin Adeyi, University of Ibadan, Nigeria Gloria Otito...Candidate working under the supervision of Akindele Oluwatosin Adeyi at the University of Ibadan. She works ...

...15-30 minute break. The method is named for the tomato or “Pomodoro” kitchen timer that the technique’...student.   The Pomodoro timer is literally a tomato! Image from Gerlos.   Tools for checking grammar...with others which makes it easier to work with collaborators. Zotero is a desktop based program that’s compatible...

...culture and include a counter, lab timer, dilution calculator, and a couple of other nice features. This app...sequence for your enzyme of interest. Lastly, the Tm calculator helps you set up an optimal PCR depending on ...apps: Findings Lab Notebook Cell Culture Split Calculator by DRDP Restriction Enzyme Tool by Promega Material...

...histidine kinase that phosphorylates a response regulator (RR). TCS-induced responses are varied; one outcome...conditions, the chromophore activates the response regulator via phosphorylation, promoting transcription of...spread across three plasmids, with the response regulator encoded in the genome. To make the system less...

...of stakeholders from industrial and commercial operators, government agencies, active researchers, as well...understanding of the societal ramifications of laboratory science, and how it may best be leveraged for...re always delighted to engage with potential collaborators and interested audiences. Please visit our team...

...sponsored the capital construction of our new BL2+ laboratory and the infrastructure capabilities to produce...our generous, sharing depositors. Over 3,000 laboratories are sharing plasmids (with 16 labs currently...helping thousands of sharing scientists from laboratories all over the world. With this reach and exposure...

...identified other advantages, including Cas13d-specific regulatory proteins and high targeting efficiency, both ...WYL domain-containing proteins may be broader regulators of Cas13d activity. This property makes WYL proteins...REPAIR Get tips for using Cas9 Transcriptional Activators Learn about Cas13a (C2c2) Characterization Resources...

...effectively contribute as part of a team.” As a seasoned educator, Kuldell also knew that the best days in the classroom... or small, receive the BioBuilder textbook, a laboratory kit, and support during the academic year from...state-of-the-art lab embedded in LabCentral’s community of innovators that opened in August 2017.  This flexible teaching...

...in Huntsville explains it, a major goal in his laboratory is to understand the function of the non-coding...effort to track down every last one of those gene regulatory proteins and the places in the genome where they... that, but the system is so flexible. Other investigators can easily adapt these vectors for use with ...

...containing the RNA pol III promoter, gRNA and pol III terminator. These relatively large cassettes (considering...Size is also a factor; the pol lll promoter and terminator need be encoded only once, and each <200 bp tRNA-gRNA...deletions of other regions, such as noncoding RNAs or regulatory elements, to examine their function. Bacteria...

...reporting and genetically encoded fluorescent voltage indicators (GEVI) are ideal tools to track bioelectric states...channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization in continuous...Use of genetically encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (...