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  1. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...transmitting and sensing electrical potential. Thankfully, there is a tool to measure these electrical ...specific types of cells, such as neuron subtypes, thanks to viral serotype specificity during delivery (...

  2. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... spent organizing, an hour is earned (Benjamin Franklin)! It’s good practice to label your plates and/...autoclaved before being disposed Happy culturing! Many thanks to our guest blogger, Sana Khan Khilji! Sana Khan...

  3. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...depositors to provide plasmid maps whenever possible. GenBank files can also be uploaded onto our plasmid pages... resources provided by the depositor including genbank files and protocols here. Articles Citing this ...

  4. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...green fluorescent protein (GFP) (6).   The Dodt and Frank Bradke laboratories later developed the 3DISCO (... system under normal and diseased conditions.  Thank you to our guest bloggers, Jae Lee and Pantelis ...

  6. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...capsid, as the engineered, recombinant genome is (thankfully) gutted of many essential viral genes. How it...blog was written by Addgenie Brian O'Neill, with thanks to Addgenie Rebecca Hutcheson for her assistance...

  8. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...know how it goes! Acknowledgements: I’d like to thank the PTA of Butler Elementary for trusting me with...with Math & Science Night, and a special thanks to Joanne Marks, Jonathan Marks, and Vanessa Bullard of...

  9. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...transfer plasmid encoded the desired transgene flanked by HIV-1 long terminal repeats (LTRs) that facilitate...Bernaudin F, Girot R, Dorazio R, Mulder GJ, Polack A, Bank A, Soulier J, Larghero J, Kabbara N, Dalle B, Gourmel...

  10. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...These primers are designed to anneal to sites that flank the insert site. A positive clone will produce a...this type of primer pair anneals to a sequence flanking the insert and one primer anneals to the insert...

  12. Plasmids 101: Protein tags

    Type
    Blog Post
    ...in-vivo biotinylation signaling peptide (Bio), flanked by hexahistidine motifs (6xHis) MBP Large Protein...Expression Vectors from the Berkeley QB3 MacroLab Eric thanks his wife, Annette Sievers, Ph.D., for her tips ...

  13. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many...018-1591-1 Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing...

  16. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...possible facing the committee and not the whiteboard. Thank everyone for the opportunity to share your research...the opportunity to realize your vision. Many thanks to our guest blogger, Erik Snapp! Erik Lee Snapp...

  17. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ..., One Drive, and G Drive. ‘Big’ data like image banks and sequencing files is likely stored in institutional...reproducibility as not all ELNs are created equal. Many thanks to our guest blogger, Rory Macneil!  Rory is founder...

  18. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct repeats which can be used for marker...through a second recombination event between the two flanking repeats. In cells where recombination has not ...

  19. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...GKPIPNPLLGLDST) has been used as a tag since 1992 (Hanke et al., 1992), and has proven itself a versatile...Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski, P., & Randall, R. E. (1992). Construction...
Showing: 181 - 200 of 497 results