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We narrowed to 494 results for: Ank

Showing: 181 - 200 of 494 results
  1. Plasmids 101: Protein tags

    Type
    Blog Post
    ...in-vivo biotinylation signaling peptide (Bio), flanked by hexahistidine motifs (6xHis) MBP Large Protein...Expression Vectors from the Berkeley QB3 MacroLab Eric thanks his wife, Annette Sievers, Ph.D., for her tips ...
  2. Bioinformatics at Addgene

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    Blog Post
    ...large formats, such as 96-well microplates, and thanks to our dedicated product and development teams,... shared resource of pipelines for genomic data. Thanks to the flexibility inherent in Nextflow’s design...
  3. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    Blog Post
    ...Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many...018-1591-1 Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing...
  4. Hot Plasmids: Fall 2024

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    Blog Post
    ...GKPIPNPLLGLDST) has been used as a tag since 1992 (Hanke et al., 1992), and has proven itself a versatile...Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski, P., & Randall, R. E. (1992). Construction...
  5. Designing Your Chalk Talk for the Academic Job Interview

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    Blog Post
    ...possible facing the committee and not the whiteboard. Thank everyone for the opportunity to share your research...the opportunity to realize your vision. Many thanks to our guest blogger, Erik Snapp! Erik Lee Snapp...
  6. Supporting Reproducibility with a Connected ELN

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    Blog Post
    ..., One Drive, and G Drive. ‘Big’ data like image banks and sequencing files is likely stored in institutional...reproducibility as not all ELNs are created equal. Many thanks to our guest blogger, Rory Macneil!  Rory is founder...
  7. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post
    ...resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct repeats which can be used for marker...through a second recombination event between the two flanking repeats. In cells where recombination has not ...
  8. Changing Labor Laws Bring Increased Postdoc Wages

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    Blog Post
    ...postdocs in the final ruling was, for a lot of us, frankly quite surprising. Consortia of higher education... changes if we wish to minimize the hurt. Many thanks to our guest blogger Gary McDowell! Gary S. McDowell...
  9. 5 Reasons to Use Reddit for Science Communication

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    Blog Post
    ...support and to monitor user replies, but I can’t thank Joanne enough for all the work she put into this.... Although I didn’t start answering until 5pm, frankly, I was typing far before five. Fortunately I have...
  10. The Challenges of Cell Culture

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    Blog Post
    ...or virology without acquiring materials from this bank. I am often asked, “What is the difference between...their identity. For example, perhaps a working cell bank has been made by a research institution (an excellent...
  11. CRISPR 101: Validating Your Genome Edit

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    Blog Post
    ... there is at least ~200 base pairs of sequence flanking the editing site on either side of the amplicon...screening by PCR may be the way to go. Design a PCR to flank your desired edit site and run the PCR product on...
  12. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...transfer plasmid encodes your insert of interest, flanked by long terminal repeat (LTR) sequences, which ...Mansour, M. R., Schwarzwaelder, K., Bartholomae, C., Hubank, M., Kempski, H., Brugman, M. H., Pike-Overzet,...
  13. March for Science

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    Blog Post
    ...staff. Many thanks to our guest blogger, Stephanie Hays. Stephanie would like to additionaly thank Isaac Plant...
  14. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post
    ...the addition of gene-specific 1 kb homology arms flanking a FLOXed neoR gene. pAAV-SEPT-Acceptor, the preferred... to deposit any new AAV tools you create. Many thanks to our guest blogger, Todd Waldman!  Todd Waldman...
  15. Site Directed Mutagenesis by PCR

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    Blog Post
    ...Also shown are the “B” restriction sites, which flank the targeted region. Upon PCR amplification, the...minor modifications to a plasmid template. Many thanks to our Guest Blogger Kristian Laursen! Kristian...
  16. Evolution of Lab Techniques

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    Blog Post
    ...Technique" in the comments section below! Many thanks to our guest blogger Krissy Lyon! Krissy Lyon is...32. PubMed PMID: 6176866. 3. Hoebeeck, Jasmien, Frank Speleman, and Jo Vandesompele. "Real-time quantitative...
Showing: 181 - 200 of 494 results