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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 709 results for: BASE;

Showing: 181 - 200 of 709 results

  1. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...process. Each variant is assigned a functional score based on its library frequency before selection compared...libraries of DNA fragments that contain variable bases (NNN) at any given codon site of interest can be... technique described in Wrenbeck et al is a PCR-based approach for generating a customizable comprehensive...primer set contains three consecutive randomized bases (NNN) at a given codon site and multiple primer ...Keith E J Tyo, and Timothy A. Whitehead. "Plasmid-based one-pot saturation mutagenesis." Nature Methods ...contain random synthesis errors, especially single basepair deletions. Therefore, Gibson assembly is better...codons that can be mutated? 88 codons, or 264 basepairs, was the maximum length tested by Wrenbeck et ...

  2. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...scientific field-based solutions or open source solutions. In comparison to paper-based record keeping,...notebooks and researcher's needs The familiar paper-based lab notebook is still a standard approach to record-keeping... or brainstorm, but we have to admit that paper-based record keeping barely copes with the growing amounts...future? As most payable software solutions have user-based pricing, it is good to know how many of your lab...

  3. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...day, you should be happy to learn that many DNA-based reagents are named for soup ingredients! From pSOUP... intracellular RNA dynamics. Aptamers are 10-100 base nucleic acid oligos that bind with high affinity...that control the expression of coding RNA. Spinach-based riboswitches can be used as stable metabolite sensors...mimic of green fluorescent protein by fluorescence-based selection and directed evolution." Journal of the...PMC4244833. Kellenberger, Colleen A., et al. "RNA-based fluorescent biosensors for live cell imaging of ...

  4. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ... modifications (~1-50 base pairs). Small edits require as little as 30-50 bases for each homology arm,...arm, but keep in mind these numbers may vary based on your locus of interest and experimental system.  Synthesizing...  Synthesizing ssODNs longer than 200 bases can be difficult, thus dsDNA plasmids are generally preferred...Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips to Improve HDR Efficiency ...

  5. CRISPR Activation: A Practical Guide

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    Blog Post
    ...latency (Bialek et al., 2016). Additionally, CRISPR-based activation can be made inducible upon environmental...through all the troubleshooting required for Cas9-based activation. CRISPRa for genome-wide studies One ...larger scale of activation allows for exploratory-based research as well. For example, CRISPRa can be used... interactions. Additionally, large scale CRISPR-based screens can be used to gain insight into protein...date his group has develop several popular CRISPR-based tools for altering DNA sequence or regulating gene...

  6. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post
    ...amino acid linker to use. These decisions were made based on known functional aspects of the protein gleaned...sequence into which it is being inserted: one thousand base pairs on each side, in fact. Later, when we transfect...precautionary measure, it’s also good to make a few single base pair mutations in crRNA target sites found within...endogenous gene tagging systems. Addgene’s CRISPR-based Protein Tagging collection includes systems for ...

  7. To Each HIS Own

    Type
    Blog Post
    ...will focus on one of the most common, purification based on the polyhistidine tag. What is the polyhistidine...tag was developed as a means to purify a protein based on the affinity of the imidazole ring of histidine...Bornhorst 2000).  One downside of polyhistidine tag-based purification, however, is the propensity for nonspecific... tags can also be used as a target for antibody based assays. Antibodies are useful tools for studying...

  9. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...Reporter signals may be electrochemical or light-based, with luminescent and fluorescent reporters being... domain induces changes in a fluorescent signal based on ligand presence or changing cellular conditions...Method Notes Quantification of a process Plate-based assays (plate reader) Detect the average amount...

  10. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...repository for DNA-based materials, including our popular kits and libraries. This DNA-based materials repository...

  13. CRISPR in the Clinic

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    Blog Post
    ...initiating a base editor-based clinical trial for SCD in 2022 and Editas Medicine starting a Cas12-based clinical... today. Below we will review some of the CRISPR-based editing therapies that are currently approved or...

  14. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...called GMAP (Gibson assembly-based modular assembly platform). GMAP is based on the Gibson Assembly method... Cas9-based AID tagging Seven years ago, Masato Kanemaki's lab successfully used the plant-based auxin-inducible...University, we are now providing a powerful CRISPR-based screening source for the Drosophila community as... it is composed of two sub-pools. The first is a base “90k library” that contains ~90,000 gRNAs that target... biosensor A pH-sensitive fluorescent biosensor-based assay system was recently deposited with Addgene...University of Zagreb, constructed a CRISPR-Cas9-based tool for targeting CpG methylation by fusing catalytically...proteins in a target cell. In addition to nucleotide-based linking strategies, post translational protein fusion...

  17. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...polymerase, the construction of specific sequences for base pair overhangs, insert and vector purification, ...specific to the insert with an additional 15-17 bases that are complementary to the overlapping vector...introduced into my inserts. However, with any PCR-based cloning technique there is a chance of introducing...

  18. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...robust protocol it does have limitations. The enzyme-based CUT&RUN method overcomes many of these limitations...interacting. The CUT&RUN method Enter CUT&RUN, an enzyme-based protocol for identifying DNA binding patterns that...sets, of which there are many readily available. Based on the success that our lab and others have had ...

  19. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...School, he developed the first lentiviral microRNA-based system for polymerase II-regulated RNA interference...virology,” he says. He was working in an HIV lab and based on that experience, soon directed himself to finding...months, the lab began a project to develop an AAV-based COVID-19 vaccine.  Vandenberghe’s work with AAVs...

  20. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...from some labs have developed fluorescent-protein based DA sensors (Figure 2). The dLight1 dopamine sensor... dLight1 sensor is a single fluorescent protein-based sensor. This sensor was engineered from a GFP variant...sensor Yulong Li’s lab has created the GRABDA sensor based on a similar strategy: a GFP variant bound to a ...
Showing: 181 - 200 of 709 results