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Showing: 181 - 200 of 711 results

  3. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...variability, and in some cases, counter-selection escape can occur: single crossover colonies can acquire... the toxin itself (leading to counter-selection escape). The rhamnose induction system is well-characterized... flexneri, Enterobacter cloacae, and Serratia marcescens and have created dozens of deletions as well ...Matthew J., et al. "Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated...enables simplified chromosomal gene replacement in Escherichia coli." Appl. Environ. Microbiol. 81.15 (2015)...Recombineering) system for genome editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed...

  4. Five Popular Model Organisms

    Type
    Blog Post
    ...difficult and time consuming. GAL4/UAS was first described in 1993 by Norbert Perrimon’s lab and has been...Transparency also allows scientists to easily observe fluorescently labelled proteins and tissues to better assess... into the worm, such as animals that express fluorescent markers (Mello et al., 1991). Genes can also ... genetic modification, such as expression of fluorescent protein or disruption of a gene, can be easily...Hesselson, Daniel, et al. "Distinct populations of quiescent and proliferative pancreatic β-cells identified...Read our quick guide to Drosophila Download the fluorescent proteins 101 eBook Read our plasmids 101 blog...

  5. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...organisms!) Escherichia coli One of the most recognizable and utilized model organisms is Escherichia coli. ...2017). Escherichia coli as a Model Organism and Its Application in Biotechnology. In Escherichia coli -...environments (Idalia & Bernardo, 2017).  Escherichia coli. Image from NIAID. E. coli is often a...and a tool-kit from the Hamdoun Lab to create fluorescent fusions in a multitude of organisms including...

  6. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...antibody worked under the conditions described above, and a short description of how the antibody performed....the submitted application, along with a brief description of the experiment. If that data was included ...ELISA — but in all cases, it contains a detailed description of the experimental conditions.     Figure 4...The submission process is easy, with simple, descriptive fields, drop-down boxes when appropriate, and...

  7. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and fluorescent-protein tagging show high correlation...First described in the 1980s, protein tags are now one of the most useful items in a scientist’s toolbox...displayed good binding efficiency in both immunofluorescence and immunoprecipitation applications, but...Plasmids Tag endogenous proteins with CRISPR Fluorescently Tag Your Protein of Interest ...

  8. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...from the event can be found below along with a description of the program beneath it. You can jump to the...the different time stamps in the description to watch any sections you’re particularly interested in, ...antibodies while FPbase curates information about fluorescent proteins so you can best apply these technologies...JoVe, and Bio-protocol all work to provide more descriptive and interactive protocols that make it much easier...Minisymposium on Reproducibility Booklet - Including descriptions of many organizations promoting reproducibility...Addgene Blog FPbase: A new community-editable fluorescent protein database ReFigure: Save Scientific Figures...

  9. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ... Y, Lacher NA, Ruesch MN. Capillary gel electrophoresis with laser-induced fluorescence of plasmid DNA...confirmations like linear and nicked plasmids, as described in this article.   Long-read sequencing Addgene...; Cook et al., 2020). CGE uses laser-induced fluorescence (CGE-LIF) for detection and can provide data...bacterial colonies using one of the detection methods described above can help ensure the isolation of the monomeric... harboring large inserts are often dimers in Escherichia coli K-12. Plasmid. 1989 Mar;21(2):138-41. doi...

  10. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...experience. Most Addgene PhD level job descriptions clearly describe that the roles do not involve bench ...submission of a job application. 3) Read job descriptions carefully Be open minded and be bold. You don...qualifications to apply for a position.  A job description is usually a wish list and the hiring manager... way to a hiring manager. Learn to confidently describe your strengths in your resume and don't be shy...

  11. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...resistance (Chen et al., 2013), fluorescence (GFP; Tzur et al., 2013), or rescue of mutant phenotypes (unc-... of lethal mutation co-selection. Only animals rescued for the conditional lethal mutation survive the...experiment, five knock-ins were recovered from seven rescued F1 animals (gel image under worm). Image courtesy...on a plate, other than the parental animal, are rescued progeny, which makes screening extremely rapid....available through Addgene. In 2019, Farboud et al. described another similar co-conversion marker (repair of...

  12. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    ...Runne-Janczy, a Product Development Manager at eScience Labs, an educational company that creates hands-on...scicomm and part two focusing on what her job at eScience Labs is like. Find all the posts in this series...don’t really know where I came from.” Working at eScience Labs, an educational company that creates hands-on...editing and searching for a job and I stumbled upon eScience by luck, by chance, by complete happenstance. ...d had at Iowa really opened the door for me at eScience.” Stay tuned for part two of Caitlin’s interview...

  13. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...transduced using only protein. Read on for a description of the lab’s iTOP protein-only transduction method...transduction by osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies...Cre- or Cas9-mediated recombination. Both primary hESCs and immortalized cells treated with iTOP efficiently...populations. After iTOP treatment, both primary (hESCs) and cultured cells showed up to 70% resistance ... frequency of biallelic mutations. Importantly, hESCs retained their stemness, indicating that iTOP may...

  14. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...for CRISPRi are described here: http://www.addgene.org/crispr/qi/ Plasmids described in  Zalatan et ...the overexpression of various metabolites in Escherichia coli or Saccharomyces cerevisiae with few techniques...www.addgene.org/browse/article/9593/ Plasmids described in Qi et al. Cell  (2013) can be found here: https...Aptamer base: a collaborative knowledge base to describe aptamers and SELEX experiments. Database, bas006...

  15. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...co-localization immunofluorescence, cell-type specificity. Viruses that only express the fluorescent proteins...Thankfully the Deisseroth constructs coexpress a fluorescent protein, which allows for easy identification...general rule of thumb, if you can detect a strong fluorescent signal (without antibody manipulation), then ... vectors for anterograde axonal tracing with fluorescent proteins in nontransgenic and cre driver mice...optogenetics plasmids! Find AAV resources! Find fluorescent protein resources!    ...

  16. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    ... - MG-2I in this case). The fluorogen is not fluorescent until it binds its protein partner thus forming...which suggests that it could be used with these fluorescent proteins. He et al also compared MG-2I-dL5** ...head-to-head with KillerRed, a genetically encoded red fluorescent photosensitizer protein that generates ROS when...Optogenetics Blog Posts Sign Up to Receive the Fluorescent Protein eBook Learn about Small Molecule Fluorophores...Fluorophores Resources on Addgene.org Visit Our Fluorescent Protein Guide Page Check out MiniSOG2 and SuperNova...

  17. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...separate DNA or transforming cells with green fluorescent protein, these activities are limited to those...Typically, if you want to make a protein – say green fluorescent protein – you first need the DNA sequence that... instructions it needs to produce that green fluorescent protein through transcription and translation...students can easily sense, whether that’s bright fluorescent proteins that they can see, enzymes that can ...Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent synthetic biology education kit." Science Advances...

  18. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...various structured surfaces. Plasmids expressing fluorescent proteins will be used to tag individual strains...within the biofilms will then be monitored using fluorescence microscopy and FACS. In an additional approach...this condition is crucial. Plasmids expressing fluorescent proteins and a new two-photon laser scanning ...plasmids containing promoter regions that activate fluorescent protein transcription in response to river contaminants...water samples at specific locations, measure fluorescent signals from the sensor, and send a signal including...

  19. Clear and Accessible Writing

    Type
    Blog Post
    ... to our website through the use of alt text to describe images in online spaces. Alt text is used by screen...example. Remember, our goal is to use words to describe the image in a way that helps visually impaired... your alt text, use all of this information to describe the image helpfully. You and your readers are ...learned a lot from listening to others' alt text descriptions, and we’d love to hear yours as well.  More ...write alt text for any image you’re struggling to describe in the results. And many journals accept alt text...

  20. AAV ddPCR Titration

    Type
    Protocol
    ... Biology Reference Introduction This protocol describes how to use droplet digital PCR (ddPCR) to titer...
Showing: 181 - 200 of 711 results