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Showing: 181 - 200 of 706 results

  1. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...quenched fluorescent ssRNA reporter is added to the reaction. Cleavage of the quenchable fluorescent RNA by...Cas13 and Cas12a, are combined with different fluorescent reporters for each enzyme. In addition, Csm6,...Csm6 and a Csm6 specific reporter (in the same fluorescent channel as the Cas13 reporter), they could significantly...papilloma virus (HPV) genome, via a crRNA. An ssDNA-fluorescently quenched (FQ) reporter, which will produce a...rate of ~1,250 cuts per second. A quantifiable fluorescent signal designates the presence of your DNA of...

  2. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...plasmid addition was first described by studies of EcoRI and Bsp6I in Escherichia coli in 1995 when introduction...systems. As with the other toxin-antitoxin systems described above, one part of the pair is more stable than...toxin-antitoxin system discovered. Unlike the systems just described, the hok/sok systems consist of three components...proof of concept, they tested a lux-mediated bioluminescence reporter in vivo in the absence of selective..., Hanna Engelberg-Kulka, and Gad Glaser. "An Escherichia coli chromosomal" addiction module" regulated...

  3. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...antibiotic’, very often we immediately imagine a prescription for a bacterial infection. While this post will...that the term ‘antibiotic’ can also be used to describe anti-parasitic drugs and anti-fungal drugs, because...development of resistance include (Figure 1): Over-prescription of antibiotics (for example, giving antibiotics...not finishing the whole course of antibiotics prescribed to them This may seem counter-intuitive considering...The take-home? Always take your medication as prescribed. How do we know which bacteria are resistant ...infection is still effectively treated. We can prescribe antibiotics for the shortest time period possible...

  4. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...variability, and in some cases, counter-selection escape can occur: single crossover colonies can acquire... the toxin itself (leading to counter-selection escape). The rhamnose induction system is well-characterized... flexneri, Enterobacter cloacae, and Serratia marcescens and have created dozens of deletions as well ...Matthew J., et al. "Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated...enables simplified chromosomal gene replacement in Escherichia coli." Appl. Environ. Microbiol. 81.15 (2015)...Recombineering) system for genome editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed...

  5. Five Popular Model Organisms

    Type
    Blog Post
    ...difficult and time consuming. GAL4/UAS was first described in 1993 by Norbert Perrimon’s lab and has been...Transparency also allows scientists to easily observe fluorescently labelled proteins and tissues to better assess... into the worm, such as animals that express fluorescent markers (Mello et al., 1991). Genes can also ... genetic modification, such as expression of fluorescent protein or disruption of a gene, can be easily...Hesselson, Daniel, et al. "Distinct populations of quiescent and proliferative pancreatic β-cells identified...Read our quick guide to Drosophila Download the fluorescent proteins 101 eBook Read our plasmids 101 blog...

  6. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...organisms!) Escherichia coli One of the most recognizable and utilized model organisms is Escherichia coli. ...2017). Escherichia coli as a Model Organism and Its Application in Biotechnology. In Escherichia coli -...environments (Idalia & Bernardo, 2017).  Escherichia coli. Image from NIAID. E. coli is often a...and a tool-kit from the Hamdoun Lab to create fluorescent fusions in a multitude of organisms including...

  7. The Addgene Antibody Data Hub

    Type
    Blog Post
    ...antibody worked under the conditions described above, and a short description of how the antibody performed....the submitted application, along with a brief description of the experiment. If that data was included ...ELISA — but in all cases, it contains a detailed description of the experimental conditions.     Figure 4...The submission process is easy, with simple, descriptive fields, drop-down boxes when appropriate, and...

  8. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and fluorescent-protein tagging show high correlation...First described in the 1980s, protein tags are now one of the most useful items in a scientist’s toolbox...displayed good binding efficiency in both immunofluorescence and immunoprecipitation applications, but...Plasmids Tag endogenous proteins with CRISPR Fluorescently Tag Your Protein of Interest ...

  9. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...from the event can be found below along with a description of the program beneath it. You can jump to the...the different time stamps in the description to watch any sections you’re particularly interested in, ...antibodies while FPbase curates information about fluorescent proteins so you can best apply these technologies...JoVe, and Bio-protocol all work to provide more descriptive and interactive protocols that make it much easier...Minisymposium on Reproducibility Booklet - Including descriptions of many organizations promoting reproducibility...Addgene Blog FPbase: A new community-editable fluorescent protein database ReFigure: Save Scientific Figures...

  10. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ... Y, Lacher NA, Ruesch MN. Capillary gel electrophoresis with laser-induced fluorescence of plasmid DNA...confirmations like linear and nicked plasmids, as described in this article.   Long-read sequencing Addgene...; Cook et al., 2020). CGE uses laser-induced fluorescence (CGE-LIF) for detection and can provide data...bacterial colonies using one of the detection methods described above can help ensure the isolation of the monomeric... harboring large inserts are often dimers in Escherichia coli K-12. Plasmid. 1989 Mar;21(2):138-41. doi...

  11. AAV ddPCR Titration

    Type
    Protocol
    ... Biology Reference Introduction This protocol describes how to use droplet digital PCR (ddPCR) to titer...

  12. Kit Free RNA Extraction

    Type
    Protocol
    ... Biology Reference Introduction This protocol describes how to extract RNA from tissues or cells without...

  13. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...experience. Most Addgene PhD level job descriptions clearly describe that the roles do not involve bench ...submission of a job application. 3) Read job descriptions carefully Be open minded and be bold. You don...qualifications to apply for a position.  A job description is usually a wish list and the hiring manager... way to a hiring manager. Learn to confidently describe your strengths in your resume and don't be shy...

  14. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...resistance (Chen et al., 2013), fluorescence (GFP; Tzur et al., 2013), or rescue of mutant phenotypes (unc-... of lethal mutation co-selection. Only animals rescued for the conditional lethal mutation survive the...experiment, five knock-ins were recovered from seven rescued F1 animals (gel image under worm). Image courtesy...on a plate, other than the parental animal, are rescued progeny, which makes screening extremely rapid....available through Addgene. In 2019, Farboud et al. described another similar co-conversion marker (repair of...

  15. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    ...Runne-Janczy, a Product Development Manager at eScience Labs, an educational company that creates hands-on...scicomm and part two focusing on what her job at eScience Labs is like. Find all the posts in this series...don’t really know where I came from.” Working at eScience Labs, an educational company that creates hands-on...editing and searching for a job and I stumbled upon eScience by luck, by chance, by complete happenstance. ...d had at Iowa really opened the door for me at eScience.” Stay tuned for part two of Caitlin’s interview...

  16. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...transduced using only protein. Read on for a description of the lab’s iTOP protein-only transduction method...transduction by osmocytosis and propanebetaine) to describe this method of protein transduction. Later studies...Cre- or Cas9-mediated recombination. Both primary hESCs and immortalized cells treated with iTOP efficiently...populations. After iTOP treatment, both primary (hESCs) and cultured cells showed up to 70% resistance ... frequency of biallelic mutations. Importantly, hESCs retained their stemness, indicating that iTOP may...

  17. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...for CRISPRi are described here: http://www.addgene.org/crispr/qi/ Plasmids described in  Zalatan et ...the overexpression of various metabolites in Escherichia coli or Saccharomyces cerevisiae with few techniques...www.addgene.org/browse/article/9593/ Plasmids described in Qi et al. Cell  (2013) can be found here: https...Aptamer base: a collaborative knowledge base to describe aptamers and SELEX experiments. Database, bas006...

  18. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...co-localization immunofluorescence, cell-type specificity. Viruses that only express the fluorescent proteins...Thankfully the Deisseroth constructs coexpress a fluorescent protein, which allows for easy identification...general rule of thumb, if you can detect a strong fluorescent signal (without antibody manipulation), then ... vectors for anterograde axonal tracing with fluorescent proteins in nontransgenic and cre driver mice...optogenetics plasmids! Find AAV resources! Find fluorescent protein resources!    ...

  19. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...separate DNA or transforming cells with green fluorescent protein, these activities are limited to those...Typically, if you want to make a protein – say green fluorescent protein – you first need the DNA sequence that... instructions it needs to produce that green fluorescent protein through transcription and translation...students can easily sense, whether that’s bright fluorescent proteins that they can see, enzymes that can ...Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent synthetic biology education kit." Science Advances...

  20. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...various structured surfaces. Plasmids expressing fluorescent proteins will be used to tag individual strains...within the biofilms will then be monitored using fluorescence microscopy and FACS. In an additional approach...this condition is crucial. Plasmids expressing fluorescent proteins and a new two-photon laser scanning ...plasmids containing promoter regions that activate fluorescent protein transcription in response to river contaminants...water samples at specific locations, measure fluorescent signals from the sensor, and send a signal including...
Showing: 181 - 200 of 706 results