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  1. Fluorescent Protein Guide

    Type
    Collection
    ... Plasmid Collections Fluorescent Protein Guide Fluorescent Proteins: Plasmids and Resources... of fluorescent proteins. Use this guide to learn more about the many applications of fluorescent proteins...Addgene Addgene Blog: Fluorescent Proteins Luciferase Plasmid Collection Fluorescent Protein Kits Allen ...this guide to learn about the applications of fluorescent proteins and to find the plasmids that are available...find plasmids for your next experiment. Browse Fluorescent Protein Plasmids by Category Empty Backbones ...of your protein of interest by fusing it to a fluorescent protein. FRET Find FRET pairs and standards to...intracellular processes with genetically encoded fluorescent biosensors. Optogenetics Guide | Optogenetics...

  2. Fluorescent Proteins: FRET

    Type
    Collection
    ... Plasmid Collections Fluorescent Proteins FRET Fluorescent Proteins: FRET FRET Pairs FRET Calibration...Luciferase can be paired with a fluorescent protein acceptor for Bioluminescence Resonance Energy Transfer ...Calibration Standards More Resources Förster (or Fluorescence) Resonance Energy Transfer (FRET) is a process...binding or conformational changes. Organic dyes, fluorescent proteins, and other molecules have been demonstrated...experiments. FRET efficiency can be measured using fluorescence microscopy or spectroscopy by exciting the donor...emission of the acceptor. Alternatively, the fluorescence lifetime (the time between excitation of the...a photon) of the donor can be measured using fluorescence lifetime imaging microscopy (FLIM) or FLIM-FRET...

  3. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal... AAV Biosensors Fluorescent biosensors are proteins designed so that their fluorescent properties are ...signal of a circularly permuted fluorescent protein (cpFP) or fluorescence resonance energy transfer (FRET... Green fluorescence indicator of K+ for optical imaging (GINKO) Genetically encoded fluorescent indicators...AMP) Yellow fluorescent cAMP indicator (Flamindo2) Genetically-encoded yellow fluorescent cAMP indicator...cyclic AMP) Red fluorescent protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based ...SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing of Fluorescent Reporters for Imaging...

  4. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Plasmid Collections Fluorescent Proteins Michael Davidson Fluorescent Protein Collection Michael Davidson...Empty Backbones Plasmid Description Industry Return to top Blue Plasmid Description Industry Return to top...top Cyan Plasmid Description Industry Return to top Green Plasmid Description Industry Return to top mEmerald... mEmerald Plasmid Description Industry Return to top Yellow Plasmid Description Industry Return to top...top Orange Plasmid Description Industry Return to top Red Plasmid Description Industry Return to top Photoactivatable...Find fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab. Plasmid...Davidson Fluorescent Protein Collection Empty Backbones Blue Cyan Green mEmerald Yellow Orange Red Photoactivatable...

  5. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ... Plasmid Collections Fluorescent Proteins In Vivo Imaging Fluorescent Proteins...photoactivatable fluorescent proteins. Plasmid...organs and whole mammals. Genetically encoded fluorescent proteins most suitable for stable imaging of ...undesirable background signals. Many near-infrared fluorescent proteins for in vivo imaging are derived from... photoreceptors (BphPs). Spectrally distinct fluorescent iRFP variants have been shown to have high brightness...of the original iRFP has resulted in a split fluorescence complementation probe used for detecting protein-protein...

  6. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... Plasmid Collections Fluorescent Proteins Subcellular Localization Fluorescent Proteins: Subcellular ... protein to a red fluorescent protein (find plasmids for cloning in our Fluorescent Proteins Empty Backbones...Collection Michael Davidson Fluorescent Protein Collection Addgene Blog: Fluorescent Proteins Do you have suggestions...Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization...localization. One of the most common is to use fluorescence microscopy to compare the localization of your...trafficking. Addgene has assembled a collection of fluorescent fusion proteins that are targeted to various ... should fuse your protein of interest with a fluorescent protein that has a different emission wavelength...

  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Return to top Fluorescent Timers Fluorescent protein variants that change their fluorescence from the blue... Plasmid Collections Fluorescent Proteins Empty Backbones Fluorescent Proteins: Empty Backbones ...Maturation is the time for fluorescence to reach half its maximal value. Structure describes the oligomeric state...Collection Michael Davidson Fluorescent Protein Collection Botman-Teusink Yeast Fluorescent Protein Collection ...Addgene Blog: Which Fluorescent Protein Should I Use? Addgene Blog: Choosing Your Fluorescent Proteins for Multi-Color...Explore empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene...Resources The following tables list many popular fluorescent proteins and empty backbones for expression and...

  8. Bacterial Expression Systems

    Type
    Collection
    ...ratio Fluorescence (GFP) Escherichia coli Gary Yellen 187836 pVoPo-01 Promoter activity Fluorescence (mCherry...activity Fluorescence (mCherry) Escherichia coli Mattheos Koffas 173481 HC-M ATP Fluorescence (GFPmut2...activity Fluorescence (mRFP1) Escherichia coli Mary Dunlop 124605 pSCM001 Cytoplasmic pH Fluorescence (mCherry... Right Connection Photoactivatable Fluorescent Proteins Fluorescent Protein Timers Return to top Protein...Anhydrotetracycline (aTc) Escherichia coli Stanley Qi 11518 pDest-527 T7-lacO Lactose/IPTG Escherichia coli Dominic...pBAD Arabinose Escherichia coli Christian Raetz 26098 pCW-LIC 3xPtac Lactose/IPTG Escherichia coli Cheryl...Lactose/IPTG Escherichia coli Cheryl Arrowsmith 43795 pDusk pR_FixK2 Blue light (470 nm) Escherichia coli Andreas...

  9. Brain Initiative Collection

    Type
    Collection
    ... with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes ready-to-use...Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter Viviana Gradinaru...An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter Viviana Gradinaru...

  10. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...phosphosite, mCherry fluorescence is restored, so interactions can be discovered by fluorescence-activated cell... (V70) (Plasmid #188537) . These reagents are described in detail in Mohler et al. (2023) and help deliver...second expression vector encoding a split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent...Phosphorylated Isomers (iSPI) The iSPI resource described in Galloway et al. (2022) is adapted from the ...Iterative Synthetically Phosphorylated Isomers (iSPI) described in: Gassaway, B. M., Li, J., Rad, R., Mintseris...in a new window) rEcoli XpS and a new pSerOTS described in: Mohler, K., Moen, J. M., Rogulina, S., & Rinehart..., 551 KB) Human Serine Phosphopeptide Library described in: Barber, K. W., Muir, P., Szeligowski, R. V...

  11. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase plasmids for gene expression assays and bioluminescent reporters....Constructs Other Highlights You may also like... Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies...Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent Imaging with Nano-lanterns Promega Plasmid Collection...Collection Luciferase is the enzyme responsible for bioluminescence found in a variety of organisms, ranging from...effects on gene expression can be measured by the luminescence output. The most popular types of luciferase...must be delivered intracellularly to measure luminescence. Gaussia luciferase is secreted and more stable...mammalian systems. ID Plasmid Luciferase Type(s) Description PI 64784 pGL3-Basic-IRES Firefly Insertion of...

  12. Plant Plasmids and Resources

    Type
    Collection
    ...choice. ID Plasmid Description Promoter Cloning Method PI Return to top Fluorescent Proteins The table...below highlights plasmids that feature fluorescent or luminescent proteins for expression in plants. You... such as the promoter or fluorescent protein of choice. Visit our Fluorescent Proteins Guide for more....Cloning Transcription Factors RNAi Empty Backbones Fluorescent Proteins Other Resources Background Addgene’s...from genome engineering and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids...Marillonnet and Johannes Stuttmann. Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly...multigene expression, gene silencing, and silencing rescue in plants - Martin Parniske. GreenGate Cloning ...

  13. Antibody Guide

    Type
    Collection
    ... is described in more detail in the Multiplex Assays section below. The following table describes the ...performs a chemiluminescent reaction in the presence of several different substrates. The luminescence produced...wells) by fluorescence activated cell sorting (FACS). Figure 7: Flow cytometry uses fluorescently-labeled...of B cells isolated from animals immunized as described above are used to create hybridomas, which produce...each antibody has two binding sites. Isotype Description Configuration and Valency Subclasses IgA Prevalent... to visualize protein location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry...primary antibody for your protein of interest, as described in the IP section above. Reverse the crosslink...

  14. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...light”): Cre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Return to top Flp Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Return to top Dre Recombinase Plasmids ID Plasmid Description Vector Type Promoter PI Additional Addgene resources...Site-Specific Recombinase Plasmids ID Plasmid Description Gene/Insert Vector Type Promoter PI Return to...target site (lox, FRT, rox, etc.) ID Plasmid Description Vector Type PI Additional Addgene resources Use...-Dependent Reporter Plasmids Incorporating a fluorescent protein into your expression vector allows you...clones, and making transgenic mice. ID Plasmid Description Gene/Insert Vector Type PI Pooled Libraries Pooled...

  15. Worm Expression Resources

    Type
    Collection
    ...backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are described below... which makes them attractive for imaging and fluorescence microscopy experiments. The C. elegans genome...search Addgene's entire collection. ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes...strains. CRISPR plasmids from John Calarco's lab as described in Heritable genome editing in C. elegans via ...genome-editing events. Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing...also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery of Custom Genetic...Approach to Better CRISPR/Cas9 Editing Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even...

  16. Brain Armamentarium

    Type
    Collection
    ...below. Plasmids ID Plasmid Description PI Viral Preps ID Viral Prep Description PI (Cargo) PI (Capsid) 230681... (Alias: CN4496) Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in... (Alias: CN2610) Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny... (Alias: CN2408) Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl... (Alias: CN2787) Expression of SYFP2 (yellow fluorescent protein) in striatal Pvalb+ neurons. Note strong... (Alias: CN3038) Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic...BGHpA (Alias: CN2700) Expression of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium ...

  17. Zebrafish Plasmid Collection

    Type
    Collection
    ... Large collections or kits are described below . ID Plasmid Description Gene/Insert PI Return to top Highlights...plasmids for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression...markers, somatic mutations, or labeling with fluorescent proteins to visualize and track clones of cells... Albert Pan and Alex Schier Labs. Multicolor fluorescent labeling in zebrafish Whole organism lineage ...to study lipid dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch ...Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes at endogenous genomic...zebrafish for studying neurodegenerative disorders. Fluorescent Protein Fusion Construct Set - Amro Hamdoun Lab...

  18. Caltech Systemic Capsids

    Type
    Collection
    ...at the bottom of this page. For more in depth descriptions of each serotype, check out Addgene's blog on...Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306 pAAV-FLEX-tdTomato... Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-FLEX-tdTomato CAG tdTomato...Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP CAG EYFP...Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control...

  19. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...overexpress various fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast...comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows...proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link...Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources External Resources...Teusink, B. (2019). In vivo characterisation of fluorescent proteins in budding yeast. Sci Rep, 9 (1):2234...n K. S. (2013) Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS...Thorn, K. S. (2004). Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. ...

  20. COVID-19 Resources

    Type
    Collection
    ...scientists. Antibodies ID Recombinant Antibody Description PI 211756 Anti-SARS-CoV-2 Nucleocapsid Protein...Plasmid Distribution to Industry page. ID Plasmid Description Industry PI Additional Addgene resources SARS-CoV... virus. It also lists several luciferase and fluorescent reporter plasmids that have been used for measuring... SARS-CoV, MERS, BAT-CoV Plasmids ID Plasmid Description Industry PI Due to export control regulations...plasmids related to these genes below. ID Plasmid Description Industry PI Additional Addgene resources ACE2...Neutralizing nanobody plasmids And more! ID Plasmid Description Industry PI Addgene blog Finding nucleic acids...Pooled Libraries Pooled Library Name Type PI Description SARS-CoV-2 Spike (S) Ectodomain and RBD Libraries...
Showing: 1 - 20 of 109 results