...option. Coupling brighter bioluminescent modules, such as luciferase-fluorescent protein fusions, to more-sensitive... routes. Image courtesy of Ute Hochgeschwender . Table 3. Descriptions of LMOs and their activity in neurons... alternative DREADD ligands. Compound 21, Deschloroclozapine (DCZ), Perlapine, and Olanzapine have all...bidirectional control of neuronal activity. Table 1. Descriptions of DREADDs and their activity in neurons DREADD...their effect, and outcome in neurons. Table 2. Descriptions of PSAMs and their activity in neurons PSAM ... PSAM plasmids ! LMOs Luminopsins (LMOs), or luminescent opsins, are opto-chemogenetic fusion proteins...Barter JW, Rossi MA, Augustine GJ, Yin HH, Hochgeschwender U (2016). Luminopsins integrate opto- and chemogenetics...

... is described in more detail in the Multiplex Assays section below. The following table describes the ...performs a chemiluminescent reaction in the presence of several different substrates. The luminescence produced...wells) by fluorescence activated cell sorting (FACS). Figure 7: Flow cytometry uses fluorescently-labeled...of B cells isolated from animals immunized as described above are used to create hybridomas, which produce...each antibody has two binding sites. Isotype Description Configuration and Valency Subclasses IgA Prevalent... to visualize protein location. Includes: Immunofluorescence (IF) Immunohistochemistry (IHC) Immunocytochemistry...primary antibody for your protein of interest, as described in the IP section above. Reverse the crosslink...

...recognizes the different fluorescent labels. The machine detects which fluorescently labeled nucleotide is...some common plasmid elements: Plasmid Element Description Origin of Replication ( ori ) DNA sequence which...the control of a non-bacterial promoter) or a fluorescent protein (that can be used to select or sort the...commercial lab strains of E. coli used today are descended from two individual isolates, the K-12 strain ...relies on the random incorporation of modified, fluorescently tagged bases onto the growing DNA strand in ...process occurs when the polymerase incorporates a fluorescently tagged nucleotide. Because these special bases...nucleotide, the reaction is halted once the fluorescently tagged base is incorporated. Sanger sequencing...

...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable... sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to...have been created by directed evolution of the Escherichia coli TadA, a tRNA adenosine deaminase. Like cytosine...customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like MS2 or PP7, can also be fused...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging...dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal...imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent CRISPR systems have been used for dynamic tracking...

...cell membrane. Microbial opsins, such as those described below, can be targeted and expressed in specific... Microbial Opsin Variants Opsin Type Variant Description Peak Response Spectra (nm) Channelrhodopsins:...dimerization. In addition to the applications described above, both LOV and Dronpa can control activity...plasmids for your next experiment. For a full description of different domains used in these plasmids, ...Glossary of Optical Switches Category Variant Description Peak Response Spectra (nm) BLUF domains bPAC ...activator FixJ in the absence of blue light. 470 Fluorescent Protein domains Dronpa based Light-inducible ...engineered from a green-to-red photoconvertible fluorescent protein 400 UVR8 domains UVR8 plant photoreceptor...

..., reverse primer pBluescriptKS TCGAGGTCGACGGTATC For pBluescript vector pBluescriptSK TCTAGAACTAGTGGATC...TCTAGAACTAGTGGATC For pBluescript vector pBMN 5' GCTTGGATACACGCCGC MMLV sequence, for inserts in pBMN retroviral vector...

...Overlay Select Fluorescent Proteins 101 August 2017 | First Edition About Fluorescent Proteins 101 ... this eBook, you’ll find tips for choosing a fluorescent protein; information on FRET, biosensors, and...

...proximal promoter, and distal promoter. Below describes the specifics of these regions in eukaryotic cells...Promoters Used in Research Promoter Expression Description CMV Constitutive Strong mammalian promoter from...Promoters used in Research Promoter Expression Description T7 Constitutive but requires T7 RNA polymerase...

...include: Plasmids 101 CRISPR 101 Viral Vectors 101 Fluorescent Proteins 101 Science Career Guide Videos Watch...techniques. Topics include: Genome Engineering Fluorescent Proteins CRISPR Viral Vectors Plasmid Technology...

...lentiviral packaging methods. The three components described above (envelope, packaging, and transfer) are ...nomenclature of the plasmid backbone is meant to be descriptive of the components found within the backbone. ...

...plasmids include: Tet- or Cre-based regulation and fluorescent fusions or reporters. Browse lentivirus plasmids...3rd generation lentiviral systems? For a full description of 2nd and 3rd generation lentiviruses, please...

...Collection of guides that describe how popular scientific techniques work...

...been made with different promoters, tags and fluorescent proteins. Thus, you can clone your gene of interest...