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We narrowed to 636 results for: ESP

Showing: 181 - 200 of 636 results

  1. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...there’s a PAM near your insertion site, the corresponding gRNA might be undesirable due to potential off-target...than SpCas9, in this case, NNGRRT and NNNNGATT, respectively. Researchers also engineered SpCas9 variants..., resulting in lower overall genome frequency. Despite the progress we’ve made, there is still a need ...

  2. Transferable Skills: Negotiation

    Type
    Blog Post
    ... on which lab jobs needed to be done (Who is responsible for making the running buffer this week?). Scientists...what is most important to each person. It can especially be difficult to think creatively when you are...create a win-win situation? Learning about and respecting cultural differences is a crucial part of understanding...

  3. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...expensive to gain access to routinely, and it can be especially challenging when trying to develop antibodies... a defined set of different trinucleotides (corresponding to different amino acids) in the regions that...further characterization and analysis. In a rapid response to the COVID-19 pandemic, the Seeger lab demonstrated...

  4. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...fluorescent proteins, it’s important to note that despite their distinction by name, chromoproteins are actually...pTOX6 express the YhaV, MqsR, and Tse2 toxins, respectively, and all encode the amilCP chromoprotein. Vectors...pTOX9 express the YhaV, MqsR, and Tse2 toxins, respectively, and encode the tsPurple chromoprotein.  Find...

  5. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...are also an important target for gene delivery, especially for the study of pain. While many new capsids...and AAV.PHP.S, that target the CNS or the PNS, respectively.  Read on to learn more about these two new ...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature biotechnology...

  6. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...without triggering a destructive inflammatory immune response. At the time, adenoviral vectors were the most...disease-causing genes, including the RPE65 gene responsible for an autosomal recessive, severe, childhood-onset...follow in the footsteps of Luxturna™. However, despite promising results in preclinical studies some potential...

  7. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...individual molecules. Poor photon budgets are a widespread issue with fluorescent proteins—even the thriftiest...student searching couch cushions for spare change, desperate to extract a few more photons from a sample. Chemical...have excellent properties inside living cells, especially for advanced imaging experiments. We continue...

  8. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...and it would have been very difficult for us to respond to such a large number of requests by ourselves...with each other, which is critically important, especially for young researchers. It was a really nice incubator...Identification of Proteins Associated with an IFNgamma-Responsive Promoter by a Retroviral Expression System for...

  9. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...Quiñones-Valles, C., Espíndola-Serna, L., Martínez-Antonio, A., Quiñones-Valles, C., Espíndola-Serna, L., & Martínez-Antonio...Solar, G., Giraldo, R., Ruiz-Echevarría, M. J., Espinosa, M., & Díaz-Orejas, R. (1998). Replication and...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...facilitate binding and cleavage of target DNA respectively. LshCas13a, on the other hand, uses only a short...(RPA) or Reverse-transcription RPA (RT-RPA), respectively. Amplified nucleotides are combined with Cas...expression. Although the collateral RNA cleavage response is well described in bacterial cells, LwaCas13a...

  11. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...Antibiotic resistance is defined as microbial survival despite exposure to antibiotics designed to kill them or...health of the patient as quickly as possible, especially if the infection is serious or life-threatening...around the disk is measured in millimeters, and corresponds to the level of susceptibility you might expect...

  12. How to Be an Excellent Trainee

    Type
    Blog Post
    ...training It’s hard to recall a lot of details, especially when you are in a new place, learning something...normal and very helpful; don’t be afraid to ask! Especially for those new to the bench, remember to let your... expect them to work around your schedule, be respectful of theirs! Show up prepared and take training...

  14. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...when bacterial cells regulate gene expression in response to changes in cell density. At the mixer, they...reviewing and commenting. Binning Singletons is especially grateful to its mentors and supporters during...was that kid who used recycled notebook paper, despite the fact that it was gray. He has worked in molecular...

  15. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...exhibit slow head growth, develop seizures, cardio-respiratory dysfunction, distinctive hand movements, and ...MECP2 duplication syndrome or Rett Syndrome, respectively. One of the main challenges facing Rett researchers...Gray’s work, are each expected to advance their respective gene therapies to clinical trials in 2021. ...

  16. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ..., but they did so without a substantial immune response or toxicity. More AAV-based CRISPR systems Smaller...with AAV, at just 1,108 and 986 amino acids, respectively, the size of both is within AAV’s packaging ...A multifunctional AAV–CRISPR–Cas9 and its host response. Nat Methods 13:868–874 . https://doi.org/10.1038...

  17. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... suggestions based on what I have found to be especially beneficial during my own graduate career – and...Attend smaller talks within the bigger conference, especially ones that are more career-focused. After these... important to realize that you have to take responsibility for your own professional development, not ...

  18. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...through video     Video abstracts about research, especially published research, have grown in popularity ...turning video views into online article downloads – especially if they are published in open access journals...studies on reducing antibiotic prescriptions for respiratory tract infections. The video abstracts provide...

  19. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...technical roles. Therefore, institutions are responsible for ensuring that either all affected scientists...is the only part of the Harvard group that has responded to the FLSA at the time of writing, and is raising... decision in FLSA, and at the time of writing responses to requests for the legal justification of this...

  20. The Challenges of Cell Culture

    Type
    Blog Post
    ...culture in the present day Cell culture is a widespread tool used in the fields of oncology, virology...of reputation that can impede collaborations, especially with industry. Today we also have the appropriate...convince people of some of the basic tenets of responsible cell culture outlined in this article. Now many...
Showing: 181 - 200 of 636 results