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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12 results for: ESP

Showing: 1 - 12 of 12 results

  1. Adenovirus Guide

    Type
    Guide
    ...disease. Innate immunity Non-specific immune response that responds to general signals of infection or damage... line are in forward or reverse orientation, respectively. Created with BioRender.com. Adenoviruses are...diseases in humans and other animals, most commonly respiratory infections, such as the common cold. The immune...elements rapidly and can mount a strong immune response quickly, which has limited their use in therapeutic... rAdV vectors were shown to elicit less immune response when administered in vivo, however deletion of...Addgene #16400) is suitable for most purposes. For especially long transgenes, the use of pAdEasy-2 (Addgene.... Due to this, and to the strong host’s immune response against the transduced cells, transgene expression...

  2. Chemogenetics Guide

    Type
    Guide
    ...chemogenetic tools are unresponsive to native ligands and are engineered to respond to small molecules that...PSAM that responded to a clinically approved drug, the PSAM domain was engineered to respond specifically... development of mutated receptors that while unresponsive to endogenous ligands, could be activated by...Gs-DREADD, rM3D, that was created by replacing the corresponding intracellular region of a turkey erythrocyte...Muscarinic-receptor based DREADDs were engineered to respond to nM concentrations of clozapine N ‐oxide (CNO... shown to be an agonist against hM4Di, and is especially attractive for use in translational studies because...of a suite of muscarinic-based DREADDs that all respond to the same ligands was that bidirectional experiments...

  3. Antibody Guide

    Type
    Guide
    ...animal, which then mounts an immune response to it. Once the immune response has developed, the antibodies ...Antibodies, produced as part of the immune system’s response to foreign bodies, are the most commonly used ...Figure 1. The two arms of the Y structure are responsible for binding the antigen, and are called the antigen...bind to a specific antigen, triggering an immune response. The stem of the Y structure and the lower part...isotype of the antibody. B cells, the immune cell responsible for producing antibodies, can change the constant...binding site. In B cells, the genetic region responsible for the variable region goes through a randomized...Different isotypes play different roles in the immune response, and can be used to increase flexibility or specificity...

  4. Optogenetics Guide

    Type
    Guide
    ... activation by light, these channels and pumps respond by opening or closing, which conducts the flow ...variety of naturally occurring microbial opsins that respond to different wavelengths of light, like blue or...opsins also initiate different electrochemical responses, such as nonspecific cation influx vs. proton ... used to trigger and study certain behavioral responses in model organisms like mice, zebrafish, and Drosophila...Opsin Variants Opsin Type Variant Description Peak Response Spectra (nm) Channelrhodopsins: cation channels...you’d pick an excitatory or inhibitory opsin, respectively. Color of activation light. There are a variety...expression of the opsin is reached. This approach is especially powerful when combined with site specific recombinase...

  5. Lentiviral Vector Guide

    Type
    Guide
    ...tethering gene that helps facilitate integration. Despite knowing the general preference for integration,...envelope genes, and/or with site-specific injections. Despite the possibility of nonspecific transduction, lentiviral...entry. Biosafety should always be considered with respect to the precise nature of experiments being performed...region where Tat binds. TAR in cis Trans-activating response element; found in the R region and acts as the...transgenes via increased nuclear export. RRE in cis Rev Response Element; he sequence to which the Rev protein ...integrase components. rev in trans Binds to the Rev Response Element (RRE) within unspliced and partially spliced...of a molecule or substance to induce an immune response in the body. in cis In the context of viral vector...

  6. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...non-pathogenic and generally do not trigger a strong immune response. These features have made AAVs the preferred choice...during production, as it carries the Cap region responsible for the production of capsid proteins. AAV2 is...to a wide range of tissues with minimal immune response, and the low risk of insertional mutagenesis. ...molecular signals of cells (commonly neurons) in response to light. The short coding sequences of channelrhodopsins...challenging due to its limited packaging capacity, especially if any additional regulatory elements are needed...entry. Biosafety should always be considered with respect to the precise nature of experiments being performed...of a molecule or substance to induce an immune response in the body. in cis In the context of viral vector...

  7. Promoters

    Type
    Guide
    ...strand that encodes codons and whose sequence corresponds to the mRNA transcript produced. The antisense...transcribed by the RNA polymerase. DNA sequences called response elements are located within promoter regions, ...phospholycerate kinase gene TRE Inducible Tetracycline response element promoter U6 Constitutive Human U6 nuclear...transcribes messenger RNA (mRNA) which is the RNA responsible for providing a stable template for the translation... RNAs (tRNA), the adaptor molecules that are responsible for bringing amino acids to the ribosome when...

  8. Addgene's eBook Collection

    Type
    Guide
    ...CRISPR 101 en español January 2024 | First Edition, trans. About CRISPR 101 en español January 2024... First Edition, trans. About Una traducción al español de nuestro libro electrónico, CRISPR 101. Close...

  9. Plan Your Experiment

    Type
    Guide
    ...manipulations used in mammalian cells and their corresponding Cas enzymes. In the next section, we will cover...transgene expression with AAVs can be long-lived, despite recombinant AAVs not integrating into the host ...necessary to optimize your delivery conditions, especially if you are using a difficult cell type. For more...

  10. CRISPR Guide

    Type
    Guide
    ...Overview Bacteria have an interesting adaptive immune response that detects and eliminates foreign DNA, which...fidelity enzymes, and their mechanisms, include: eSpCas9(1.1) - weaken interactions between the HNH/RuvC...sequence The sequence within the gRNA that is responsible for Cas9 binding, it does not include the 20 ...

  11. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...entry. Biosafety should always be considered with respect to the precise nature of experiments being performed...of a molecule or substance to induce an immune response in the body. in cis In the context of viral vector...

  12. Molecular Biology Reference

    Type
    Guide
    ..., and guanine (abbreviated to A, T, C, and G respectively) that are organized into a double stranded helix... selective pressure to retain the plasmid DNA, despite the added replication burden, as bacteria without...
Showing: 1 - 12 of 12 results