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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22 results for: ESP

Showing: 1 - 20 of 22 results

  1. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...antigen to demonstrate the expected antibody dose response.... an antibody against the target to show a dose response. Sharing speeds science. We believe that sharing...curve . If you don’t see any color change or dose response, your antibody concentration may have been too...reaction is saturated and you don't see any dose response, your antibody concentration may have been too...

  2. Protocol - How to Design Primers

    Type
    Protocol
    ...The main property of primers is that they must correspond to sequences on the template molecule (must be...template strand). However, primers do not need to correspond to the template strand completely; it is essential...essential, however, that the 3’ end of the primer corresponds completely to the template DNA strand so elongation...

  3. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...replication. 5’LTR 5’ long terminal repeat. RRE Rev response element. A.3 Related Products The following plasmids...Avoid stretches of 4 or more nucleotide repeats, especially repeated Ts because polyT is a termination signal...Optimal Puromycin Concentration Each cell line responds differently to puromycin selection. Addgene strongly...

  4. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...UV box (be sure to wear proper UV protection - especially for your eyes!), remove it from any gel tray ...bottom and sides with the razor blade. This is especially important during the DNA purification step, as...

  5. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...depicts the sequence as a series of colored peaks corresponding to one of the four nucleotide bases. This is...your sequencing company or core facility The corresponding primer sequence used for each reaction Tips ...

  6. Ligation Independent Cloning

    Type
    Protocol
    ...sequence which must be built into the 5’ end of the respective primers. Below we use pNIC28-Bsa4 as an example... T4 Pol in the presence of dCTP (not dGTP), corresponding to the first base removed by the restriction...

  8. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...the volume of homogenized cell solution that corresponds to 50 cells: Volume of homogenized cell solution... be sure to look thoroughly within each well, especially in the corner of the well as cells tend to settle...

  9. DNA Quantification

    Type
    Protocol
    ...Repeat for each sample. Notes: Keep in mind that despite the accuracy of the NanoDrop, if two consecutive...

  12. Gibson Assembly Protocol

    Type
    Protocol
    ...stitch” together several oligos. This technique is especially useful for introducing promoters, terminators...

  19. Pouring LB Agar Plates

    Type
    Protocol
    ...plates with colored marker - particular colors correspond to particular antibiotics. Position the flame...
Showing: 1 - 20 of 22 results