...antigen to demonstrate the expected antibody dose response.... an antibody against the target to show a dose response. Sharing speeds science. We believe that sharing...curve . If you don’t see any color change or dose response, your antibody concentration may have been too...reaction is saturated and you don't see any dose response, your antibody concentration may have been too...

...The main property of primers is that they must correspond to sequences on the template molecule (must be...template strand). However, primers do not need to correspond to the template strand completely; it is essential...essential, however, that the 3’ end of the primer corresponds completely to the template DNA strand so elongation...

...replication. 5’LTR 5’ long terminal repeat. RRE Rev response element. A.3 Related Products The following plasmids...Avoid stretches of 4 or more nucleotide repeats, especially repeated Ts because polyT is a termination signal...Optimal Puromycin Concentration Each cell line responds differently to puromycin selection. Addgene strongly...

...UV box (be sure to wear proper UV protection - especially for your eyes!), remove it from any gel tray ...bottom and sides with the razor blade. This is especially important during the DNA purification step, as...

...depicts the sequence as a series of colored peaks corresponding to one of the four nucleotide bases. This is...your sequencing company or core facility The corresponding primer sequence used for each reaction Tips ...

...sequence which must be built into the 5’ end of the respective primers. Below we use pNIC28-Bsa4 as an example... T4 Pol in the presence of dCTP (not dGTP), corresponding to the first base removed by the restriction...

...and ~25 kDa corresponding to the heavy chain (HC) and light chain (LC) proteins, respectively. There should...

...the volume of homogenized cell solution that corresponds to 50 cells: Volume of homogenized cell solution... be sure to look thoroughly within each well, especially in the corner of the well as cells tend to settle...

...Repeat for each sample. Notes: Keep in mind that despite the accuracy of the NanoDrop, if two consecutive...

...anyone else associated with Addgene, can be held responsible for your use of the information contained in ...

...antigen to demonstrate the expected antibody dose response....

...anyone else associated with Addgene, can be held responsible for your use of the information contained in ...

...higher transformation efficiencies with less DNA, especially when using highly competent cells. If you used...

...instructions). Note: For many ligation reactions, especially if using "high concentration" ligase, 5min at...

...stitch” together several oligos. This technique is especially useful for introducing promoters, terminators...

...each QuickSeal tube. The first 3 mL collected corresponds to the 60% phase and can be discarded. The fractions...

... temperature around 70°C and is the molecule responsible for driving the DNA synthesis. Sterile dH 2 O...

... the transgene may have reduced growth rates, especially if that transgene is toxic. Eventually, the rapidly...

...plates with colored marker - particular colors correspond to particular antibiotics. Position the flame...

...look as you expected. When cloning by PCR, it is especially important to run the product on a gel. This allows...