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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 671 results for: FIE

Showing: 181 - 200 of 671 results

  1. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...after landslide, only adding more stones to the field. Fortunately, our bodies have been fighting cancer...tumor; chemokine-aided activation of APCs produces modified dendritic cells (DCs) and macrophages (M1 and ...still many important questions in this exciting field of research. One of the most important: How will...successful, cancer immunotherapy must aspire to be a field of healthcare as dynamic as cancer itself. Many...

  2. Ligation Independent Cloning

    Type
    Protocol
    ...restriction enzymes (e.g. BsaI), which cut at a specified distance from their recognition sequence. This... end will now look like this: Note: Elute your purified digestion and PCR products with sterile water ...purification . Step 5: Create Insert Overhangs Treat your purified PCR product with T4 Pol in the presence of dCTP...

  3. CRISPR Cheat Sheet

    Type
    Blog Post
    ... crisp new CRISPR material for you!  The CRISPR field moves fast. That’s why we’re bringing you a brand...functional change in the DNA sequence. Base Editors: Modified versions of CRISPR nucleases that make single ...convert adenosine to inosine. Activate/Repress: Modified versions of CRISPR nucleases that can’t cut DNA...

  4. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ... 75,000 pathogenic genetic variants have been identified in humans and cataloged in the ClinVar database...engineered prime editing guide RNA (pegRNA) that both specifies the target site and contains the desired edit ...of RNA stability. Using a CRISPRi screen, they identified a protein that enhances prime editing in mammalian...forward to following new advances in this evolving field! Find prime editing plasmids! Find plasmids from...

  5. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous organisms (E. coli, ...and later C. elegans) for the first time. After Chalfie’s work, scientists really started to take notice...Nobel Prize in chemistry was awarded to Shimomura, Chalfie, and Tsien for their work on GFP. This great honor...

  6. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...The field of induced pluripotent stem cells (iPSCs) has been around for 10 years. In that time, scientists...Cesar A., and Gustavo Mostoslavsky. "The evolving field of induced pluripotency: Recent progress and future...Kotton Four Factors that Differentiate the Stem Cell Field Additional Resources on Addgene.org Visit our ... Check out the Yamanaka paper that started the field of iPSCs Find gene-specific plasmids (including ...

  7. Deep Dive: qPCR

    Type
    Blog Post
    ... Most general-use protocols, unless otherwise specified, were likely developed on the ABI 7000 series....fairly equal, an assumption that is not always verified before use. If you’re using the ΔΔCT method and... analysis methods that pair well with slightly modified CT analysis, so pick one and use it! Tempting ...and Miller KS. 2003. Accurate and statistically verified quantification of relative mRNA abundances using...

  8. Reflections and Looking Ahead

    Type
    Blog Post
    ...Request, a service that dramatically expands and simplifies your viral vector options. Almost any AAV transfer..., V., Fotouhi, M., Alqazzaz, M., Southern, K., Alshafie, W., Baker, M. R., Ball, A. R., Callahan, D., ...K. J., Houston, D. W., Kumaran, R., Rego, M., Schofield, C., … Laflamme, C. (2024). A consensus platform...

  10. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...better research. CRISPR is an incredibly fast-moving field, and we want to make it easy for you to keep up ...design programmable epigenome editing tools, and the field has certainly taken notice! Our newest page in the...In mammalian systems, Cas13a degrades only the specified RNA target. Bonus: It displays similar on-target...

  11. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...expressed from a separate plasmid. This system amplifies the intensity of the fluorescent signal and enables...scaffold. To increase expression, Tanenbaum et al. modified the GCN4 sequence to increase its alpha-helical...chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule tracking Caveats ...

  12. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ...it is essential to delve deeply into your chosen field, don't shy away from looking beyond its confines...exposes you to discussions beyond your immediate field, fostering a diverse range of perspectives and sparking...approaches Engaging in mindful meditation, taking field trips, and spending time outdoors in nature or visiting...

  13. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...fluorescent biosensor. Image from Chesterfield et al., 2020     Chesterfield et al., ACS Synthetic Biology...plants.  FnCas12a, unlike other Cas12a nucleases identified, functions at temperatures up to 43 °C and can...

  14. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...Imperial College London for students in this emerging field. It has gained over 170 members in its first year...Synthetic Biology and exciting new technologies in the field. One of the main aims of the society is to bridge...hopes to help young scientists interested in this field excel and develop their career skills for positions...

  15. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...by three of the biggest names in the CRISPR-Cas9 field – Jennifer Doudna, Feng Zhang, and Keith Joung. ...community has clearly made a huge impact on this field. Advances in genome engineering & CRISPR technology... cutting edge science in increasingly important field.  Interested in Addgene's Keystone Poster?  We'll...

  16. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...brought out in a Kavli Foundation Round Table. In any field, good communication skills help when it comes to...everyone – not just to others working in your own field. Alternative careers are now the norm for those ...you are ready, tell your friends or others in the field where to find it. A good way to reach others in ...

  17. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...purification. Electrophoresis uses an electrical field to move the negatively charged DNA through an agarose...temperature for 20-30 mins, until it has completely solidified. Pro-Tip If you are in a hurry, the gel will ...well, instead of diffusing in the buffer. Once solidified, place the agarose gel into the gel box (electrophoresis...

  18. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...adeno-associated viral vector (AAV) samples and created a simplified process, termed viral genome sequencing (VGS)...viral genome sequencing workflow. Using this simplified approach, we sequenced over 100 AAV vector preparations...routinely producing AAV. We believe that this simplified sequencing and analysis approach can be easily...

  19. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...strategy to determine the binding kinetics of a purified protein involves immobilization on a solid support...One possibility is covalent modification of the purified target protein with the vitamin biotin. The interaction...apparent affinity for the ligand, especially if the modified amines are located near the ligand binding site...

  20. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ... For an estimate on availability date or to be notified when a particular catalog item is available for... the vector name and serotype you’d like to be notified about. Controls Plasmid Serotype Name 114472...pAAV-hSyn-DIO-mCherry 59462  AAV2  pAAV-CAG-tdTomato (codon diversified) 37825  AAV8*, AAV9*  pAAV-CAG-GFP 50465 ...
Showing: 181 - 200 of 671 results