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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 472 results for: FLU;

Showing: 181 - 200 of 472 results

  1. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ...element (tyrosine sulfation consensus sequence, fluorophore, or APEX2), allowing for quantitative analysis... Since the anti-GFP nanobody is able to bind fluorescent proteins derived from A. victoria the functionalized...fused with GFP, YFP, mCerulean, or other related fluorophores. Find all of the nanobody toolkit plasmids here...technologies to quantify proteins in tissues, cells, and biofluids. Recently, Sam Myers, a Scientist in the group...

  2. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...mCherry fluorescent protein followed by a stop codon and the coding sequence for a green fluorescent protein...representation of cell-free system, capable of producing fluorescent proteins and enzymes. Image from Moore et al....validated through monitoring the expression of fluorescent proteins and oxytetracycline enzymes. Next, this...

  3. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...recent contribution to the field is a series of fluorescence resonance energy transfer (FRET)-based calcium...with a solution: they devised self-associating fluorescent domains whose association is disrupted in the...Robust red FRET sensors using self-associating fluorescent domains. ACS Chem Biol. 2013 Oct 18;8(10):2133...et al. MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor. PLoS One. 2013 Dec 2;8(12):e82009...

  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...with Rosella, a pH-sensitive fluorescent biosensor A pH-sensitive fluorescent biosensor-based assay system...expression constructs fused with epitopes or fluorophores, generate expression vectors with various promoters...DsRed is relatively pH-insensitive, while SEP fluoresces at cellular pH (7.0) but not at vacuolar pH (...environments that inactivate SEP and leave only DsRed to fluoresce (1). With variants that can be targeted to specific...tagging event. Commonly used sequences, including fluorescent tags, a floxed Cbr-unc-119 selectable marker,...the mAID vectors come with mCherry2 or mClover fluorescent proteins allowing you to screen for fusions. ...

  5. Hot Plasmids - January 2023

    Type
    Blog Post
    ...speed of light over the last decade, but the fluorescent proteins (FPs) necessary for many of these applications...light and electron microscopy and observed high fluorescence resilience to these preparations. After crystalizing...    Fig. 4: hfYFP retains folding and fluorescence in high molarity GdnHCl, at high temperatures...!   Campbell, B.C., et al. Chemically stable fluorescent proteins for advanced microscopy. Nat Methods...

  6. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ...Genetically encoded voltage indicators (GEVIs) change fluorescence in real time when neurons or muscle cells transmit...However, technical difficulties such as small fluorescence changes or a requirement for very high excitation...sensors can detect action potentials in worms with fluorescence changes of up to 128% per 100 mV in the pharynx.... elegans membranes, which might beneficially influence the rhodopsin properties. Find these voltage biosensors...

  7. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...Choose the Brightest Fluorescent Protein Resources on Addgene.org Find Fluorescent Protein Plasmids Check...mRNA this displacement leads to a change in the fluorescent signal: untranslated RNAs appear yellow, and ..., which contains the tethering sites for the fluorescent proteins and is driven by an inducible promoter...

  8. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...cytometry controls we’ll discuss are single color, fluorescence minus one (FMO), and isotype. You should always...should have a single color control for every fluorophore you intend to use in your panel. Using single...(C) with APC-Cy7.   FMOs FMO stands for fluorescence minus one. It is used as a negative control ...have the same Ig subclass, and be in the same fluorophore (e.g., if you are staining for anti-mouse Ly6C...

  9. Deep Dive: qPCR

    Type
    Blog Post
    ...Agarose gel  LUX fluorogenic primers Low Single self-quenching fluorescent molecule connected to...normalize for that. Passive reference dyes are inert fluorescent dyes that are not dependent on amplification ... It is possible to multiplex qPCR when using fluorescent probe-based chemistry - theoretically, up to ... Tip🔥 The ABI machines automatically record fluorescent data on all channels - helpful if you forget ...

  10. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...are now available for fluorescent reporting and genetically encoded fluorescent voltage indicators (GEVI...anion-conducting channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization...sensor and a mutated version of the pHLuorin fluorescent protein. This GEVI features increased brightness...

  11. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...agglomeration of fluorescent proteins into one particle within the cell, the lab fused the red fluorescent protein...different bee species. BTK can be used to introduce fluorescent markers to visualize localization and distribution...division counting (DCDC), relies on the assembly of fluorescent proteins into one bright particle that segregates...

  12. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...found at the same site, is 2’-fluoro (2’-F), which substitutes a fluorine for the hydroxyl group at the...simple LED light. Lighting up your gRNA with fluorescent dyes In a scientific world where it often seems...seems like everything glows, why not spread the fluorescence to your gRNAs? Dye labeling the RNA in your ...

  13. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ... antigen-retrieval, and permeabilization will influence how the antibody interacts with its target and...conjugated to a detection substrate, such as a fluorophore, or a conjugated secondary antibody that will...of primary antibodies conjugated to different fluorophores or a panel of primaries that was raised in different...isotype or species, then you can choose different fluorophore-conjugated secondary antibodies specific to each...

  14. Optogenetics AAV Preps

    Type
    Collection
    ...regulatory element Fluorophore GFP Red-wavelength fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength...Blue-wavelength fluorescent protein Activity Cre dependent Flp dependent Cre and Flp dependent Constitutive...

  15. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...chemiluminescent or fluorescent detection, like horseradish peroxidase (HRP) or Alexa Fluor dyes, respectively...want to set up your blot with a polyvinylidene difluoride (PVDF) membrane, which is sturdier and retains...fresh chemiluminescent substrate or imaging your fluorescent probe. Then, if you don’t see any signal, you...

  16. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...photostable than fluorescent proteins, making them ideal for single-molecule fluorescence techniques. After...deliver ASAP5 to the cortex and imaged voltage fluctuations in awake behaving mice through two-photon microscopy...used to measure signal along the dendrite. D) Fluorescence signal as a function of the distance between...

  17. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...Green or Taqman for signaling. Sybr Green is a fluorescent dye that works by binding to all double-stranded...while Taqman uses an intercalating dye, with a fluorescent probe and quencher to quantify specific amplicons...materials, and is not particularly sensitive. The fluorescent signal can be affected by AT-rich DNA and the... Sybr Green chemistry. Blue arrows represent fluorescent signaling in the presence of dsDNA. Image courtesy...

  18. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ..., if your protein is fused with a fluorescent protein), fluorescence microscopy can theoretically be used... We screen the wells in groups as they become confluent, taking both early- and late-arising clones, and...Addgene.org Check Out Our Protocols Pages Browse Fluorescent Protein Plasmids Find Mammalian Expression Vectors...

  20. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...mammalian expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6...pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3...pBad-EBFP2 - Bacterial expression of EBFP2 blue fluorescent protein 35S-eGFP-nosT - Transient expression ... with multiple cloning sites Browse our full Fluorescent Protein (FP) Guide and the Michael...
Showing: 181 - 200 of 472 results