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We narrowed to 476 results for: FLU;

Showing: 181 - 200 of 476 results

  1. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ...element (tyrosine sulfation consensus sequence, fluorophore, or APEX2), allowing for quantitative analysis... Since the anti-GFP nanobody is able to bind fluorescent proteins derived from A. victoria the functionalized...fused with GFP, YFP, mCerulean, or other related fluorophores. Find all of the nanobody toolkit plasmids here...technologies to quantify proteins in tissues, cells, and biofluids. Recently, Sam Myers, a Scientist in the group...

  2. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...recent contribution to the field is a series of fluorescence resonance energy transfer (FRET)-based calcium...with a solution: they devised self-associating fluorescent domains whose association is disrupted in the...Robust red FRET sensors using self-associating fluorescent domains. ACS Chem Biol. 2013 Oct 18;8(10):2133...et al. MagFRET: The First Genetically Encoded Fluorescent Mg(2+) Sensor. PLoS One. 2013 Dec 2;8(12):e82009...

  3. Hot Plasmids - January 2023

    Type
    Blog Post
    ...speed of light over the last decade, but the fluorescent proteins (FPs) necessary for many of these applications...light and electron microscopy and observed high fluorescence resilience to these preparations. After crystalizing...    Fig. 4: hfYFP retains folding and fluorescence in high molarity GdnHCl, at high temperatures...!   Campbell, B.C., et al. Chemically stable fluorescent proteins for advanced microscopy. Nat Methods...

  4. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ...Genetically encoded voltage indicators (GEVIs) change fluorescence in real time when neurons or muscle cells transmit...However, technical difficulties such as small fluorescence changes or a requirement for very high excitation...sensors can detect action potentials in worms with fluorescence changes of up to 128% per 100 mV in the pharynx.... elegans membranes, which might beneficially influence the rhodopsin properties. Find these voltage biosensors...

  5. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...Choose the Brightest Fluorescent Protein Resources on Addgene.org Find Fluorescent Protein Plasmids Check...mRNA this displacement leads to a change in the fluorescent signal: untranslated RNAs appear yellow, and ..., which contains the tethering sites for the fluorescent proteins and is driven by an inducible promoter...

  6. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...cytometry controls we’ll discuss are single color, fluorescence minus one (FMO), and isotype. You should always...should have a single color control for every fluorophore you intend to use in your panel. Using single...(C) with APC-Cy7.   FMOs FMO stands for fluorescence minus one. It is used as a negative control ...have the same Ig subclass, and be in the same fluorophore (e.g., if you are staining for anti-mouse Ly6C...

  7. Deep Dive: qPCR

    Type
    Blog Post
    ...Agarose gel  LUX fluorogenic primers Low Single self-quenching fluorescent molecule connected to...normalize for that. Passive reference dyes are inert fluorescent dyes that are not dependent on amplification ... It is possible to multiplex qPCR when using fluorescent probe-based chemistry - theoretically, up to ... Tip🔥 The ABI machines automatically record fluorescent data on all channels - helpful if you forget ...

  8. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...probes with different functionalities, such as fluorescence or triggering new cellular behavior like degradation...Tetracycline (Tet) transactivator to increase fluorescent protein expression C234-E25: attB2r-SNAP-attB3...HaloTag Ligands HaloTag Reversible binding of fluorescent ligands to HaloTag PCV-Cas9, Cas9-PCV HUH-tag... fused to other effectors from nanobodies to fluorescent proteins. So, I hope you will take full advantage...

  9. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...found at the same site, is 2’-fluoro (2’-F), which substitutes a fluorine for the hydroxyl group at the...simple LED light. Lighting up your gRNA with fluorescent dyes In a scientific world where it often seems...seems like everything glows, why not spread the fluorescence to your gRNAs? Dye labeling the RNA in your ...

  10. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ... antigen-retrieval, and permeabilization will influence how the antibody interacts with its target and...conjugated to a detection substrate, such as a fluorophore, or a conjugated secondary antibody that will...of primary antibodies conjugated to different fluorophores or a panel of primaries that was raised in different...isotype or species, then you can choose different fluorophore-conjugated secondary antibodies specific to each...

  11. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...are now available for fluorescent reporting and genetically encoded fluorescent voltage indicators (GEVI...anion-conducting channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization...sensor and a mutated version of the pHLuorin fluorescent protein. This GEVI features increased brightness...

  12. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...agglomeration of fluorescent proteins into one particle within the cell, the lab fused the red fluorescent protein...different bee species. BTK can be used to introduce fluorescent markers to visualize localization and distribution...division counting (DCDC), relies on the assembly of fluorescent proteins into one bright particle that segregates...

  13. Worm Expression Resources

    Type
    Collection
    ... which makes them attractive for imaging and fluorescence microscopy experiments. The C. elegans genome...This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large ...and GFP fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available with and without...Approach to Better CRISPR/Cas9 Editing Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even...

  14. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... Science has deposited plasmids to generate fluorescently tagged proteins of key cellular structures in... Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization Plasmids... collection (Link opens in a new window) of fluorescently-tagged human induced pluripotent stem cell (...plasmids researchers will be able to generate fluorescently tagged proteins of key cellular structures in...

  15. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...chemiluminescent or fluorescent detection, like horseradish peroxidase (HRP) or Alexa Fluor dyes, respectively...want to set up your blot with a polyvinylidene difluoride (PVDF) membrane, which is sturdier and retains...fresh chemiluminescent substrate or imaging your fluorescent probe. Then, if you don’t see any signal, you...

  16. Lentiviral Prep Service

    Type
    Collection
    ...expresses EGFP for easy visualization via direct fluorescence. Morris 115644 Morris CellTag Barcode Library...expresses EGFP for easy visualization via direct fluorescence. Morris 115645 Morris CellTag Barcode Library...expresses EGFP for easy visualization via direct fluorescence. Morris Control Lentivirus Browse our list of...based on plasmids in our repository that express fluorescent proteins. ID Name Insert Antibiotic Resistance...

  17. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...photostable than fluorescent proteins, making them ideal for single-molecule fluorescence techniques. After...deliver ASAP5 to the cortex and imaged voltage fluctuations in awake behaving mice through two-photon microscopy...used to measure signal along the dendrite. D) Fluorescence signal as a function of the distance between...

  18. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...Green or Taqman for signaling. Sybr Green is a fluorescent dye that works by binding to all double-stranded...while Taqman uses an intercalating dye, with a fluorescent probe and quencher to quantify specific amplicons...materials, and is not particularly sensitive. The fluorescent signal can be affected by AT-rich DNA and the... Sybr Green chemistry. Blue arrows represent fluorescent signaling in the presence of dsDNA. Image courtesy...

  19. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ..., if your protein is fused with a fluorescent protein), fluorescence microscopy can theoretically be used... We screen the wells in groups as they become confluent, taking both early- and late-arising clones, and...Addgene.org Check Out Our Protocols Pages Browse Fluorescent Protein Plasmids Find Mammalian Expression Vectors...
Showing: 181 - 200 of 476 results