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We narrowed to 64 results for: FLU;

Showing: 1 - 20 of 64 results

  1. Fluorescent Protein Guide

    Type
    Collection
    ... Plasmid Collections Fluorescent Protein Guide Fluorescent Proteins: Plasmids and Resources... of fluorescent proteins. Use this guide to learn more about the many applications of fluorescent proteins...Addgene Addgene Blog: Fluorescent Proteins Luciferase Plasmid Collection Fluorescent Protein Kits Allen ...this guide to learn about the applications of fluorescent proteins and to find the plasmids that are available...find plasmids for your next experiment. Browse Fluorescent Protein Plasmids by Category Empty Backbones ...of your protein of interest by fusing it to a fluorescent protein. FRET Find FRET pairs and standards to...intracellular processes with genetically encoded fluorescent biosensors. Optogenetics Guide | Optogenetics...

  2. Fluorescent Proteins: FRET

    Type
    Collection
    ... Plasmid Collections Fluorescent Proteins FRET Fluorescent Proteins: FRET FRET Pairs FRET Calibration...Alternatively, the fluorescence lifetime (the time between excitation of the fluorophore and emission of ...position and orientation of the fluorophores through linkers, fluorescent protein expression level and maturation...Calibration Standards More Resources Förster (or Fluorescence) Resonance Energy Transfer (FRET) is a process...non-radiatively transferred from an excited donor fluorophore to an acceptor. Because this process is extremely...extremely sensitive to the distance between the fluorophores at the single-nanometer scale, scientists can...binding or conformational changes. Organic dyes, fluorescent proteins, and other molecules have been demonstrated...

  3. Fluorescent Protein Guide: Biosensors

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    Collection
    ... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal...levels and flux Nanosensor detection of an immunoregulatory tryptophan influx/kynurenine efflux cycle. PLoS...Measure autophagic flux by pH-sensitive fluorescence (pMRX-IP-GFP-LC3-RFP) An Autophagic Flux Probe that Releases...Reporters and Resources Fluorescent biosensors are proteins designed so that their fluorescent properties are ...signal of a circularly permuted fluorescent protein (cpFP) or fluorescence resonance energy transfer (FRET...orange-emitting fluorescent proteins. Nat Commun. 2017 Sep 5;8(1):431. Wolf Frommer Calcium Orange fluorescent calcium... Green fluorescence indicator of K+ for optical imaging (GINKO) Genetically encoded fluorescent indicators...

  4. Michael Davidson Fluorescent Protein Collection

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    Collection
    ...Plasmid Collections Fluorescent Proteins Michael Davidson Fluorescent Protein Collection Michael Davidson...Find fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab. Plasmid...Davidson Fluorescent Protein Collection Empty Backbones Blue Cyan Green mEmerald Yellow Orange Red Photoactivatable... collection of ORFs tagged with a variety of fluorescent proteins. The majority of the ORFs are of mammalian... corresponding empty backbone with different fluorescent tags for you to create fusion proteins with your...

  5. Fluorescent Protein Guide: In Vivo Imaging

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    Collection
    ... Plasmid Collections Fluorescent Proteins In Vivo Imaging Fluorescent Proteins...photoactivatable fluorescent proteins. Plasmid...organs and whole mammals. Genetically encoded fluorescent proteins most suitable for stable imaging of ...undesirable background signals. Many near-infrared fluorescent proteins for in vivo imaging are derived from... photoreceptors (BphPs). Spectrally distinct fluorescent iRFP variants have been shown to have high brightness...of the original iRFP has resulted in a split fluorescence complementation probe used for detecting protein-protein...

  6. Fluorescent Protein Guide: Subcellular Localization

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    Collection
    ... Plasmid Collections Fluorescent Proteins Subcellular Localization Fluorescent Proteins: Subcellular ... protein to a red fluorescent protein (find plasmids for cloning in our Fluorescent Proteins Empty Backbones...Collection Michael Davidson Fluorescent Protein Collection Addgene Blog: Fluorescent Proteins Do you have suggestions...Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization...localization. One of the most common is to use fluorescence microscopy to compare the localization of your...trafficking. Addgene has assembled a collection of fluorescent fusion proteins that are targeted to various ... should fuse your protein of interest with a fluorescent protein that has a different emission wavelength...

  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Return to top Fluorescent Timers Fluorescent protein variants that change their fluorescence from the blue... Plasmid Collections Fluorescent Proteins Empty Backbones Fluorescent Proteins: Empty Backbones ...Collection Michael Davidson Fluorescent Protein Collection Botman-Teusink Yeast Fluorescent Protein Collection ...Addgene Blog: Which Fluorescent Protein Should I Use? Addgene Blog: Choosing Your Fluorescent Proteins for Multi-Color...Explore empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene...Resources The following tables list many popular fluorescent proteins and empty backbones for expression and...experiment. Tables are organized by color of the fluorescent protein. The content for this page was originally...

  8. Bacterial Expression Systems

    Type
    Collection
    ... Right Connection Photoactivatable Fluorescent Proteins Fluorescent Protein Timers Return to top Protein...interactions using fluorescence microscopy. FRET relies on the principle that when a donor fluorophore is excited...will be able to detect fluorescence both from the donor and the acceptor fluorophore by only exciting the...ratio Fluorescence (GFP) Escherichia coli Gary Yellen 187836 pVoPo-01 Promoter activity Fluorescence (mCherry...activity Fluorescence (mCherry) Escherichia coli Mattheos Koffas 173481 HC-M ATP Fluorescence (GFPmut2...activity Fluorescence (mRFP1) Escherichia coli Mary Dunlop 124605 pSCM001 Cytoplasmic pH Fluorescence (mCherry...Promoter activity Fluorescence (turbo-365) Mycobacterium sp. Tanya Parish 26156 pMV306hsp+FFluc Promoter activity...

  9. Antibody Guide

    Type
    Collection
    ... curve with known amounts of protein. Fluorophores Fluorophores are light-sensitive molecules which emit...cell or tissue sample. Figure 6: Immunofluorescence, in which fluorophore-conjugated primary antibodies ...label cells in a sample. Immunofluorescence (IF) This method uses fluorophore-conjugated antibodies to...IF uses fluorophore color to differentiate between the targets of interest, if your fluorophore is bound...wells) by fluorescence activated cell sorting (FACS). Figure 7: Flow cytometry uses fluorescently-labeled...the fluorophores used is important. It is best to inversely pair the brightness of the fluorophore with...If a bright fluorophore is on an abundant target, it obscures fainter signals. Fluorophores can emit signals...

  10. Brain Initiative Collection

    Type
    Collection
    ...genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter Viviana Gradinaru...An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter Viviana Gradinaru...Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal... AAV production plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward...

  11. Genetic Code Expansion

    Type
    Collection
    ... Mehl 197576 pAcBac1-Mb-Fluoro-Phe B5 Pyl Fluoro-Phe B5 M. barkeri fluorinated phenylalanine derivatives... Mehl 197577 pAcBac1-Mb-Fluoro-Phe D6 Pyl Fluoro-Phe D6 M. barkeri fluorinated phenylalanine derivatives...5FTrp)RS Methanogenic archaeon 4-Fluoro-L-Tryptophan (4FTrp), 5-Fluoro-L-Tryptophan (5FTrp) Bacterial TAG...7FTrp)RS Methanogenic archaeon 6-Fluoro-L-Tryptophan (6FTrp), 7-Fluoro-L-Tryptophan (7FTrp) Bacterial TAG...TAG Chang Liu 85484 pDule-tfmF A65V S158A tri-fluoromethyl-phenylalanine synthetase M. jannaschii family...Wenshe Liu 137908 PylRS-AS PylRS M. mazei fluorophenylalanine derivatives Bacterial TAG Wenshe Liu 137976...163916 pXPR-SF61 SF5Phe tRNA synthetase para-Pentafluorosulfanyl-Phenylalanine Bacterial TAG Thomas Huber ...

  12. Luciferase Plasmid Collection

    Type
    Collection
    ...donor and HaloTag labeled with a fluorophore as the acceptor. LumiFluors: EGFP-NanoLuc ( GpNLuc ) and LSSmOrange-NanoLuc...Nanoluc®. CalFlux VTN : An intracellular calcium sensor created by fusing NanoLuc® and Venus fluorophore to ...Constructs Other Highlights You may also like... Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies...Addgene's collection include: Firefly luciferase (Fluc) Renilla luciferase (Rluc) Gaussia luciferase (Gluc...luciferase expression Stephen Tapscott 83281 pAAV-CAG-FLuc Firefly CAG AAV expression of firefly luciferase...luciferase Mark Kay 105532 pAAV.CMV.ffLuciferase.SV40 Firefly CMV AAV expression of firefly luciferase James Wilson... Wilson 101156 T7-CMVtrans-FFLuc-polyA Firefly T7 Expression of firefly luciferase from a cell-free mammalian...

  13. Botman-Teusink Yeast FP Collection

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    Collection
    ...overexpress various fluorescent markers and tag your proteins of interest with fluorescent proteins in yeast...comprehensive collection of yeast codon-optimized fluorescent proteins (FPs) in a variety of plasmids allows...proteins of interest with yeast codon-optimized fluorescent proteins. These vectors are based on the pFA6a-link...Teusink Lab Materials Kurt Thorn Lab Materials Fluorescent Protein Plasmids & Resources External Resources...Teusink, B. (2019). In vivo characterisation of fluorescent proteins in budding yeast. Sci Rep, 9 (1):2234...n K. S. (2013) Improved blue, green, and red fluorescent protein tagging vectors for S. cerevisiae. PLoS...Thorn, K. S. (2004). Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. ...

  14. Zebrafish Plasmid Collection

    Type
    Collection
    ...plasmids for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression...markers, somatic mutations, or labeling with fluorescent proteins to visualize and track clones of cells... Albert Pan and Alex Schier Labs. Multicolor fluorescent labeling in zebrafish Whole organism lineage ...to study lipid dynamics in vivo. Bimolecular fluorescence complementation (BiFC) tools - Marco Morsch ...Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes at endogenous genomic...consisting of plasmids with four promoters, six fluorophores, and empty vectors designed to streamline the...zebrafish for studying neurodegenerative disorders. Fluorescent Protein Fusion Construct Set - Amro Hamdoun Lab...

  15. Control AAV Preps

    Type
    Collection
    ... 5, 8 James M. Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase Constitutive 8 James M. Wilson...Browse our control AAV collection, including fluorescent proteins, spaghetti monster, and more. Viral...Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These AAV can...EF1a/nEF GFAP and variants Synapsin TBG Other Fluorophore/Tag Green Red None Other Cre-dependent color ...non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG...pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 CAG GFPsm_myc (does not fluoresce) Cre dependent 1 Loren Looger 98928 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40...pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 CAG Ruby2sm-Flag (does not fluoresce) Cre dependent 1, 5 Loren Looger 45185 AAV-EF1a-BbTagBY...

  16. Microbiology Resources

    Type
    Collection
    ...bacterial expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection...Kits for Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology... Enterococcus sp. Geobacillus sp. Haemophilus influenzae Helicobacter pylori Listeria monocytogenes Mycobacterium...Plasmids for Viruses Species Dengue Hepatitis Influenza Mumps virus Rubeola (measles) West Nile virus ...systems) and therefore supplied as one item. Fluorescent Proteins FRET-based biosensors : E. coli expression...expression - Pertz Lab Bacterial Fluorescence Labeling : Broad-Range Rainbow Vectors - Barbier Lab Cloning...

  17. Brain Armamentarium

    Type
    Collection
    ... (Alias: CN4496) Expression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in... (Alias: CN2610) Expression of SYFP2 (yellow fluorescent protein) in striatal direct pathway medium spiny... (Alias: CN2408) Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl... (Alias: CN2787) Expression of SYFP2 (yellow fluorescent protein) in striatal Pvalb+ neurons. Note strong... (Alias: CN3038) Expression of SYFP2 (yellow fluorescent protein) in striatal and lateral septum cholinergic...BGHpA (Alias: CN2700) Expression of mTFP1 (cyan fluorescent protein) in striatal indirect pathway medium ...BGHpA (Alias: CN2237) Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium ...

  18. Plant Plasmids and Resources

    Type
    Collection
    ...Return to top Fluorescent Proteins The table below highlights plasmids that feature fluorescent or luminescent... such as the promoter or fluorescent protein of choice. Visit our Fluorescent Proteins Guide for more....Cloning Transcription Factors RNAi Empty Backbones Fluorescent Proteins Other Resources Background Addgene’s...from genome engineering and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids...Marillonnet and Johannes Stuttmann. Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly...

  19. CRISPR Guide

    Type
    Collection
    ...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable... sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to...: Epigenetics Visualizing Genomic Loci Using Fluorophores CRISPR imaging has numerous advantages, including...customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like MS2 or PP7, can also be fused...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging...dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal...imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent CRISPR systems have been used for dynamic tracking...

  20. CRISPR Plasmids for Genomic Visualization

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    Collection
    ...catalytically inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9...into a customizable DNA labeler compatible with fluorescence microscopy in living cells. Alternatively, gRNAs...specific RNA-binding proteins (RBPs) tagged with fluorescent proteins to visualize targeted genomic loci. ...live cell imaging. Compared to techniques like fluorescence in situ hybridization (FISH), CRISPR imaging... the chromatin dynamics in living cells. The fluorescent CRISPR system has been used for dynamic tracking...
Showing: 1 - 20 of 64 results