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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14 results for: FLU;

Showing: 1 - 14 of 14 results

  1. Antibody Guide

    Type
    Guide
    ... curve with known amounts of protein. Fluorophores Fluorophores are light-sensitive molecules which emit...cell or tissue sample. Figure 6: Immunofluorescence, in which fluorophore-conjugated primary antibodies ...label cells in a sample. Immunofluorescence (IF) This method uses fluorophore-conjugated antibodies to...IF uses fluorophore color to differentiate between the targets of interest, if your fluorophore is bound...wells) by fluorescence activated cell sorting (FACS). Figure 7: Flow cytometry uses fluorescently-labeled...the fluorophores used is important. It is best to inversely pair the brightness of the fluorophore with...If a bright fluorophore is on an abundant target, it obscures fainter signals. Fluorophores can emit signals...

  2. Chemogenetics Guide

    Type
    Guide
    ... , K + influx Neuronal activation Magnus et al., 2019 PSAM Q79G,Q139G Gly PSEM 22S Cl - influx Neuronal... , K + influx Neuronal activation Magnus et al., 2011 PSAM L141F,Y115F Gly PSEM 89S Cl - influx Neuronal...K + influx Neuronal activation Björefeldt et al., 2024 LMO11 GeNL_SS ChRmine CFz Na + , K + influx Neuronal...IPD. Binding of the cognate PSEM allows for the influx of Cl - ions, and inhibits neuronal activity. PSAM...potassium-selective IPD. Binding of the cognate PSEM allows for influx of Na + and/or K + ions, and activates neuronal...PSAM4 Gly Varenicline, uPSEM 792, uPSEM 817 Cl - influx Neuronal inhibition Magnus et al., 2019 PSAM4 5HT3... 2011 PSAM L141F,Y115F 5HT3 PSEM 89S Na + , K + influx Neuronal activation Magnus et al., 2011 LMOs Luminopsins...

  3. CRISPR Guide

    Type
    Guide
    ...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable... sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to...: Epigenetics Visualizing Genomic Loci Using Fluorophores CRISPR imaging has numerous advantages, including...customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like MS2 or PP7, can also be fused...fused to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging...dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal...imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent CRISPR systems have been used for dynamic tracking...

  4. Molecular Biology Reference

    Type
    Guide
    ...the control of a non-bacterial promoter or a fluorescent protein to select or sort the cells by visualization...level of transcription driven by that promoter. Fluorescent Protein Plasmids , Luciferase Plasmids Viral ...relies on the random incorporation of modified, fluorescently-tagged bases during in vitro DNA replication...standard bases (dNTPs) are tagged with a different fluorophore so they can be distinguished from one another... DNA because the ultimate goal is to have a fluorescently-tagged nucleotide at each position in the DNA...DNA strands are separated by size, read using fluorescence detection, and the sequence outputted in the...Figure 4). Like Sanger, NGS utilizes modified, fluorescently-tagged nucleotides. During Illumina NGS, a long...

  5. Modular Cloning Guide

    Type
    Guide
    ...PhytoBrick parts. MoBiFC - Modular Bimolecular Fluorescence Complementation Toolkit Plant Expression Benjamin...Field 50 plasmids for assembling bimolecular fluorescence complementation experiments to analyze protein-protein...Volker Sieber Plasmids with control elements and fluorescent reporters to be used in combination with the ...synthetic terminators, protein purification tags and fluorescence proteins for use in E. coli for a variety of...Christopher Dupont 95 plasmids including backbones, fluorescent proteins, protein tags, and signal peptides, ...

  6. Optogenetics Guide

    Type
    Guide
    ... responses, such as nonspecific cation influx vs. proton efflux. Researchers have used genetic engineering...excited by blue light and permits nonspecific cation influx into the cell when stimulated. Channelrhodopsin...activator FixJ in the absence of blue light. 470 Fluorescent Protein domains Dronpa based Light-inducible ...engineered from a green-to-red photoconvertible fluorescent protein 400 UVR8 domains UVR8 plant photoreceptor...

  7. Addgene's eBook Collection

    Type
    Guide
    ...Overlay Select Fluorescent Proteins 101 August 2017 | First Edition About Fluorescent Proteins 101 ... this eBook, you’ll find tips for choosing a fluorescent protein; information on FRET, biosensors, and...localization; an introduction to non-protein fluorophores; and much more! Close About Overlay Select Viral...

  8. Educational Resources

    Type
    Guide
    ...include: Plasmids 101 CRISPR 101 Viral Vectors 101 Fluorescent Proteins 101 Science Career Guide Videos Watch...techniques. Topics include: Genome Engineering Fluorescent Proteins CRISPR Viral Vectors Plasmid Technology...

  9. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...resistance is not the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection...marker. Instead of selection by an antibiotic, fluorescence-activated cell sorting (FACS) is used to sort...

  10. Lentiviral Vector Guide

    Type
    Guide
    ...resistance is not the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection...marker. Instead of selection by an antibiotic, fluorescence-activated cell sorting (FACS) is used to sort...

  11. Guide to Using Pooled Libraries

    Type
    Guide
    ...accurately evaluate which library components are influenced by selection, so it’s essential that you have...

  12. Molecular Cloning Techniques

    Type
    Guide
    ...different promoters, tags, selection markers, and fluorescent proteins to fit a variety of experimental conditions...

  13. Plan Your Experiment

    Type
    Guide
    ... selectable marker (antibiotic resistance or fluorophore) are also relevant. Delivery Using Plasmids The...
Showing: 1 - 14 of 14 results